Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"patient derived cells"”.
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Genipin-crosslinked electrospun collagen nanofibers
… engineering deals with creating tissues, using patient-derived cells, in order to restore, maintain or improve existing tissue function. In traditional tissue engineering, tissues are grown in-vitro prior to insertion in the body, and thus a scaffold is needed to support and guide the cells …
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Investigating the role of mitochondria in the hypoxia response using in vitro and in vivo models
… oxygenation and metabolism in human cells, partly by controlling the expression of respiratory chain complex subunits. In cancer, CHCHD4 is required for tumour growth in vivo, with overexpression in a range of cancers correlating with increased tumour progression, the hypoxia gene …
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Investigating Cell-Type-Specific Vulnerability in Mitochondrial Disease Using Stem Cell-Derived Neuronal Models
… In this thesis, I developed human stem cell-derived neuronal models for two nuclear-encoded mitochondrial disorders that impair mitochondrial DNA (mtDNA) replication and translation. The first model examined the selective vulnerability of GABAergic inhibitory interneurons to mutations in the …
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Three-dimensional Hyaluronan-based Hydrogels Designed for Breast Cancer Spheroid Formation
In vitro culture of cancer cells is traditionally performed with two-dimensional (2D) culture, which fails to model the in vivo tumour cell phenotype and microenvironment. Hyaluronan (HA) is upregulated in breast cancer stroma and thus an excellent starting material in which to culture breast …
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Exploring the influence of adipokines on neuronal function in Alzheimer’s disease
… death. In this study, human neuroblastoma SHSY5Y cells were differentiated using retinoic acid to demonstrate neuronal-like characteristics which was confirmed by immunocytochemistry (ICC). The pre-adipocytes 3T3-L1 cells, were chemically differentiated to exhibit adipocyte-like characteristics …
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An investigation of the function of adaptor protein complex 4 (AP-4)
… fractions from wild-type and AP-4-depleted cells, three ubiquitously expressed transmembrane cargo proteins, ATG9A, SERINC1 and SERINC3, were found to be mislocalised in AP-4-deficient cells. Two novel cytosolic AP-4 accessory proteins, RUSC1 and RUSC2, were also identified. Further …
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Interrogating Cancer Metabolism using Novel Imaging Techniques
… enable the proliferation and survival of cancer cells in changing microenvironments. Metabolic changes can be interrogated using non-invasive metabolic imaging techniques, which enable the investigation of tumour metabolism in vivo and the identification of imaging biomarkers. Hyperpolarised 13C …
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MUTATION OF REGNASE-1 CAUSES PRIMARY IMMUNODEFICIENCY ASSOCIATED WITH AUTO-INFLAMMATORY DISEASE
… increased susceptibility to infection. Some PID patients may also have autoimmune and autoinflammatory manifestations. In many cases, PIDs are monogenic disorders that follow Mendelian inheritance and mutations in more than 250 genes have been shown to cause PIDs. However, in the majority of PID …
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Cytosolic Nucleic Acid Sensing and Senescence in a Patient-Derived Model of Progressive Multiple Sclerosis
… characterised by an attack by adaptive immune cells against myelin. For reasons not yet fully understood, most patients eventually transition to a disease stage known as progressive MS (P-MS), involving a shift from adaptive immune disease mechanisms to a CNS-compartmentalised innate immune …
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ALK in the pathogenesis of cancer
… its endogenous expression is limited to neuronal cells during neonatal development, although resistance to ALK-targeted therapy has been observed. In this thesis I explore potential mechanisms of resistance to the ALK inhibitors that have been approved for ALK+ non-small cell lung cancer (NSCLC) …
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Investigation of the effect of disulfiram on the chemoresistance and invasiveness in pancreatic cancer cells
… dismal. One reason for this is because most PDAC patients are asymptomatic and end up being diagnosed after the cancer has advanced to a late stage. Another major obstacle in PDAC management is that PDAC cells are highly resistant to currently available anticancer drugs and the resistant cells …
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Investigating the Genomics and Heterogeneity of Childhood Non-Hodgkin Lymphoma
… high-intensity chemotherapy. Unfortunately, many patients who relapse have a dismal prognosis, with long-term survival less than 30% for relapsed BL patients in particular. Furthermore, the side effects of chemotherapy on children are often severe, rendering intensive chemotherapy infeasible in …
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CHCHD2 and the tumor microenvironment in glioblastoma
… regarded as putative energy sensors within cells, also play a central role as signaling organelles. Retrograde signaling occurring from mitochondria to the nucleus allows mitochondria to sense intracellular metabolic stress imposed by the extracellular tumor microenvironment and respond …