Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"paratope"”.
-
On Epitope-Paratope Interactions of Emerging to Endemic Viruses
… that bind virus epitopes via the antibody’s paratope domain in an epitope-paratope interaction (EPI). EPIs are key aspects of the dynamic interplay between the virus and the host immune response to neutralize the virus. Studying EPIs enables us to better understand viral pathogenesis, map …
-
Using data to characterise the relationship between nanobody sequence, structure and function
… properties of antigen-contacting residues (the `paratope'), show Nb paratopes are more variable than those of Abs. This is true for both the distribution of paratope residues across the domain, and the types of residues used at interfaces. Notably, Nbs deviate from the `loops = paratope' …
-
Construction and mutagenesis of an anti-insulin single chain Fv (scFv) using protein engineering techniques: Identification of peptide mimotopes which inhibit scFv-insulin binding.
… (Selectide technology) which bind to the paratope of an anti-insulin monoclonal antibody. These peptides inhibit insulin from binding to the anti-insulin antibody, indicating that they share at least part of the paratope for insulin. The 50% inhibitory concentrations (IC50) of certain …
-
Engineering developable miniprotein ligand scaffolds
… framework sequences and four or five distinct paratopes, to determine the impact of topology, paratope, and framework sequence on evolvability and developability. We discovered several library designs that were both evolvable and developable, producing numerous binders across four distinct …
-
Learning the Language of Antibody Hypervariability Through Biological Property Prediction
… specificity, as well as identification of its paratope. The scalability of AbMAP newly enables large-scale analysis of human antibody repertoires. We find that the AbMAP representations of individual repertoires have remarkable overlap, more so than can be discerned by sequence analysis. Our …
-
Engineering of an anti-PAI-1 antibody with tissue specific activation
… DARPins, which I selected against the MEDI-579 paratope. Using human and rodent transcriptomics data, three proteases, renin, hepsin and MMP-7, were identified that appear restricted to the kidney and have a specific cleavage signature. Activation of the pro-antibody with the renin protease has …
-
Computer simulation of titration behavior in proteins
… complexes which differ minimally in epitope and paratope structure, but have widely varying association constants, are studied. In addition to electrostatic techniques, results of contact mapping and molecular surface area and volume calculations aid in the understanding of the differing …
-
Replaying Life's Tape With Intraclonal Germinal Center Evolution
… mutations. CryoEM revealed that central paratope of clone 2.1 is largely optimal in its ability to bind chIgY, but it can be further improved by changing the periphery of its contact residues or leverage allosteric fine-tuning to optimize antibody geometry. In the fifth part of this …
-
Interrogating structure-function relationships in the pathogenesis and treatment of human disease : insights into the development of therapeutics for emergent tropical viruses
… antibodies to identify key features of epitope-paratope interactions. In the context of Ebola virus, I use this analytical framework to identify two novel epitopes on the surface of the trimeric coat glycoprotein which are highly constrained, such that antibodies targeting these regions are …
-
Ein Protein für neue Aufgaben: die cytosolische PH-Domäne des Cytohesin-1 als Paratop und als Substrat für Translokationen
… von E. coli, weshalb nicht auf bindende Paratope aus der Bibliothek selektiert werden konnte. Nach Verbesserung der Translokationseigenschaften von PH-Domänen sollte sich das phage-display-Verfahren zur Selektion bindender Mutanten fortsetzen lassen. 6.2. Die PH-Domäne als Substrat für …
-
The resurgence of structure in deep neural networks
Machine learning with deep neural networks ("deep learning") allows for learning complex features directly from raw input data, completely eliminating hand-crafted, "hard-coded" feature extraction from the learning pipeline. This has lead to state-of-the-art performance being achieved across …