Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 66 for “"paralog"”.
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Characterization of U2AF26, a Paralog of the Splicing Factor U2AF35
… sequence motifs. In the present work U2AF26, a paralog of the conventional U2AF35, has been studied. U2AF26 shares 84% primary amino acid identity with U2AF35, suggesting functional homology. However, U2AF26 has two amino acid substitutions in ribonulceoprotein consensus sequence-2 (RNP-2) and …
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Incomplete paralog compensation and splicing regulation by TRA2A and TRA2B in cancer
Paralogous genes are widespread in the human genome and frequently retain overlapping functions, enabling cells to withstand the loss of one gene without compromising viability. In cancer, this functional redundancy provides resilience against genomic instability but also creates therapeutic …
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INVESTIGATION OF THE NOVEL ANTITERMINATION MECHANISM OF LOAP, A NUSG SPECIALIZED PARALOG
… operons, and CRISPR arrays. Specialized NusG paralogs, such as LoaP, RfaH, or UpxY, are widespread in bacteria and likely form their own unique antitermination complexes. RfaH, the only well-studied paralog, binds to a paused TEC via a specific non-template DNA sequence, then prevents Rho …
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Molecular Characterization of pFGE, the Paralog of the C-α-Formylglycine-generating Enzyme
… fehlt. Im Genom der Deuterostomia wurde ein Paralog des FGE, genannt pFGE, gefunden. Das humane pFGE ist zu 62,1% homolog und zu 47,1% identisch mit FGE. Seine biologische Funktion ist unbekannt. Das Ziel der vorliegenden Arbeit war, die strukturellen und funktionellen Eigenschaften von pFGE …
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Molecular and Immunohistochemical Identification of a Sodium Hydrogen Exchanger-2C (Nhe2C) Paralog in the Gills of Marine Longhorn Sculpin (Myoxocephalus Octodecemspinosus)
… While, in the future, studying syntenic NHE paralogs in the NHE2 family of longhorn sculpin could give us an evolutionary perspective to homologous NHEs in higher vertebrates.
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Determining therapeutically actionable genetic interactions in human cancer at scale using multiplexed CRISPR screening
… machine-learning classifier to predict SL among paralog pairs, prioritising those with clinical relevance and therapeutic tractability (Chapter 3). Pooled screens were conducted using the Synergy library across 12 cancer cell lines, including those derived from the colon, kidney, stomach, and …
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The IN4MER CRISPR/Cas12a Multiplex Knockout Platform and Its Applications
… of synthetic lethal interactions, such as paralog pairs. In recent years, a couple of studies have conducted dual-gene knockout experiments on paralog pairs using different approaches to identify synthetic lethal interactions. In this study, we conducted a meta-analysis of CRISPR genetic …
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Expanding cancer therapy options through genome-scale identification of synthetic lethal paralogs
… CRISPR-Cas9 library called paired guide RNAs for paralog genetic interaction mapping (pgPEN). pgPEN targets over 2,000 human paralogs, or duplicated genes. We applied pgPEN to lung and cervical cancer cell lines and found that 12% (n = 122) of paralog pairs exhibited synthetic lethality in at …
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Selectivity in subunit composition of EnaNASP tetramers
… in the others. Unique modes of regulation and paralog-specific interacting partners point to potential differences in the activity and function of EnaNASP proteins. The function of this family of proteins relies on their ability to form tetramers via a highly conserved tetramerization domain …
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The Role of TNRC6 in RNA Interference
… in many different processes. There are three paralogs in mammalian cells, TNRC6A, TNRC6B, and TNRC6C. These paralogs share approximately 40% amino acid sequence identity. Whether the paralogs have unique or redundant functions is unclear. Much is known about the mechanisms of cytoplasmic RNAi …
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Essentiality and Regulation of Deoxyhypusination in Trypanosoma brucei
… eukaryotes, trypanosomatids encode two divergent paralogs of DHS (DHSp: Tb927.1.870 and DHSc: Tb927.10.2580), only one of which (DHSc) contains the key catalytic lysine. I showed that both DHS genes are essential for growth of bloodstream-form T. brucei using conditional gene knockouts, further …
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DROSOPHILA CLASS-I MYOSINS THAT CAN IMPACT LEFT-RIGHT ASYMMETRY HAVE DISTINCT ATPASE KINETICS
… the molecular mechanisms of two class-I myosin paralogs, myosin-1C (myo1C) and myosin-1D (myo1D), that can impact Drosophila Left-Right asymmetry. De novo overexpression of either paralog in symmetric tissues results in opposing tissue rotation, dependent on the paralog. De novo overexpression …
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Biases and Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens
… these never-essentials are highly enriched for paralogs, suggesting that functional redundancy masks the detection of a substantial number of genes. Finally, I will describe our efforts to investigate functional buffering among approximately 400 candidate paralog pairs using CRISPR/enCas12a …
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The <i>Drosophila</i> T-box Transcription Factor Midline Functions within the Insulin/AKT and c-Jun-N-terminal Kinase Signaling Pathways to Regulate Interomatidial Bristle Formation and Cell Survival
… effect. We next assayed Mid and H15, a paralog of Mid, via Western blot analysis and report that Mid exhibits a nucleocytoplasmic distribution pattern that is altered within the <em>mid</em>-RNAi mutant while H15 was found exclusively within the cytoplasmic fraction. This opens the …
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MFSD7C: A Solute Carrier Linking Heme and Calcium in Mitochondrial Energy Metabolism
… and angiogenesis. MFSD7C and its closely related paralog MFSD7B, have been proposed to function as heme transporters, however this hypothesis was challenged by several groups. We now show that MFSD7C localizes to mitochondria where it interacts with the electron transport chain complex. Loss of …
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Structural Insights into Mycobacteriales Galactan Biosynthesis
… characterize a previously undescribed GlfT2 paralog from Rhodococcus equi, which we term ReqGlfT3. We confirm its galactofuranosyl transferase activity and identify the production of β(1,3) and β(1,5) linkages. These findings offer new insights into GlfT2 and related polymerizing …
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Isolation and characterization of an RNA polymerase III encoded gene of Pinus radiata and its use in pine transformation
… the characterization of 5Spr20, a pine SS rDNA paralog. 5Spr20 differs from all published SS rDNA sequence& dnd is therefore a novel pine gene. Analyses of its sequence using bioinformatics revealed that it is capable of initiating biologically active transcripts and 5Spr20 is therefore a …
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Role of subfunctionalized MYB paralogs in the evolution of pigmentation patterning in Clarkia
… pigments, always involves subfunctionalized MYB paralogs that each control the initiation of pigmentation in a specific pattern element. These early studies have mostly focused on the mechanism for patterning, though, and few have looked at the evolution and diversification of this complex …
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Molecular Evolution of Anthocyanin Biosynthesis in Morning Glories
… and adaptive evolution proceeded along both paralog lineages. In the second chapter, I determine the molecular genetic basis of a flower color transition that is associated with change in pollinator attraction in morning glories. A regulatory change in a branching gene in the flavonoid …
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Molecular Genetic Analysis of Non-Catalytic Pol IV and V Subunits
… For instance, NRPB9b but not its 97% similar paralog, NRPB9a is required for RdDM. Likewise, Pol IV and Pol V-specific 7th largest subunits are very similar yet have different involvements in RdDM. In some of the non-catalytic subunit mutants of Pol IV, 24nt siRNA accumulation is not …
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