Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"paracellular permeability"”.
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Computational Modeling of Claudin-15 Paracellular Channels
Tight junctions (TJs) regulate paracellular permeability in epithelial tissues through claudin protein assemblies, yet the molecular basis of strand flexibility, pore architecture, and ion selectivity for cation-selective claudin-15 (Cldn-15) remains incompletely defined at atomic resolution. This …
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The Role of Arginine Vasopressin Receptor 2 in Microvascular Hyperpermeability During Severe Sepsis and Septic Shock
… that V2R activation induces vascular hyperpermeability. The hypothesis was tested using a well-characterized ovine model of MRSA sepsis and various in vitro cell-based assays with human lung microvascular endothelial cells (HMVECs). Results demonstrated that the treatment of septic sheep …
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Calf intestinal health: assessment and dietary interventions for its improvement
… in calf gut health as measured by intestinal paracellular permeability in response to age, failure of passive transfer, and intestinal location. Analysis of fecal score data indicated that risk for intestinal disease changed hyperbolically from 5 to 35 days with a risk peak around 13 days of …
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Regulation of cadherin adhesion at intercellular junctions
… cell-cell junctions, wound healing dynamics, and paracellular permeability. Adhesion frequency measurements were used to demonstrate the allosteric regulation of cadherin adhesive function. In this thesis, measured kinetics of cadherin-mediated intercellular adhesion demonstrated quantitatively …
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Dietary carbohydrates influence the structure and function of the intestinal alpha-glucosidases
… promoted higher tight junction formation and permeability compared to glucose and sucrose at 12 hours. Contrary to this finding, paracellular permeability increased with maltose. In addition to barrier function of small intestinal enterocytes, a metabolomics study was conducted using …
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An <i>in vitro</i> human 3D co-culture model to study endothelial-astrocyte interactions
… claudin-5, and P-glycoprotein and similar paracellular permeability coefficients to fluorescent-dextrans (70 kDa).<br></br><br></br>Using the developed model, the effect of endothelial cells on the AQP4 expression in HA was investigated. While the localisation of astrocytic AQP4 did not …
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The effects of ethanol and aspalathus linearis on immortalized mouse brain endothelial cells (bEnd5)
… (ECs), regulates BBB transport by regulating the permeability both transcellularly and through their paracellular junctions, by structures called tight junctions (TJs) that are composed of proteins. The aim of this study was to investigate the in vitro effects of ethanol (EtOH) and fermented …
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Development of in vitro infant intestinal models to assess responses to infant formulae
… with the neonatal gut characterized by higher permeability than in adults. The aim of this thesis was to develop and validate in vitro models that mimic the infant intestinal barrier. Once developed, these models were employed to study epithelial responses to digested human milk and infant …
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Gene Delivery to the Blood-Brain Barrier for Ischemic Stroke Therapy
… cells) efficiently. A lucifer yellow apparent permeability (LY P<sub>app</sub>) method was developed to determine that DNA NPs with 0.03% P84 was a safe formulation for TJ integrity. However, this formulation did not result in increased BDNF secretion compared to untreated cells. To determine …