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Showing 1 to 3 of 3 for “"pKM101"”.
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Characterization of The Role of The Pkm101-Encoded T4Ss Outer Membrane Core Complex In Substrate Transfer, Pilus Biogenesis, and Recipient Cell Contact
… (T4SS) encoded on the <em>Escherichia coli</em> pKM101 and R388 conjugative plasmids and the <em>Agrobacterium tumefaciens</em> <em>virB</em> operon are composed of 12 subunits arranged in an architecturally similar fashion. Structural studies of the T4SS from R388 (Trw<sub>R388</sub>) …
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Development of Chimeric Type Iv Secretion Systems For Transfer of Heterologous Substrates Across The Gram-Negative Cell Envelope
… proteins. I next tested the <em>E. coli</em> pKM101-encoded T4SS, which is known to transfer DNA substrates, for the capacity to deliver heterologous protein substrates to <em>E. coli</em> recipients. Using the Cre-recombinase reporter assay for translocation (CRAfT), I showed that pKM101 …
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Pseudomonas chlororaphis (pJP4) als effizienter Rhizosphäre-Kolonisierer: Untersuchung fitness-relevanter Faktoren und Etablierung einer in situ-Detektionsmethode
… übt vermutlich eine ähnliche Funktion in pKM101-determinierten Pili aus. Zellfraktionierung pJP4-tragender P. chlororaphis-Zellen und Detektion mit spezifischen Antiseren deuten darauf hin, dass TrbC und TrbF Haupt- und Nebenkomponente pJP4-determinierter Pili sind. Bei TrbH handelt es …