Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"pHluorin"”.
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Biochemistry and physiological role of otoferlin
… vitro zu testen.Zusätzlich etablierten wir den pHluorin-basierten optischen Test der Exozytose in IHZ. Wir exprimierten pHluorin gekoppelt an den vesikulären Glutamat-Transporter 1 (VGLUT1). PHluorin ist eine pH-Wert abhängige GFP-Variante, die bereits in Neuronen verwendet wird. Seine …
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Dissecting Synaptic Vesicle Endocytosis
… utilized the sensitivity and robustness of the pHluorin assay of synaptic vesicle recycling to probe the control of endocytosis spatially and in response to varying stimulation conditions. We examined the role of the large GTPases dynamin 1 and dynamin 3 in synaptic vesicle endocytosis; which …
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Genetic Dissection of Synaptic Vesicle Endocytosis
… of vesicular glutamate transporter fused to pHluorin, is unperturbed. Synaptic vesicle endocytosis in the absence of dynamin 2 was assessed at both room temperature and 32 oC. At both temperatures, my results reveal that synaptic vesicle recycling functions independent of dynamin 2 but also, …
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Non-Canonical Axonal Insulin Receptor Signaling Drives Aversive Olfactory Learning
… through AWCON cell body calcium imaging and pHluorin imaging of synaptic glutamate release, and ask how ist-1 modifies these mechanisms during learning. I show that these molecular and neuronal mechanisms result in behavior changes during biased random walk chemotaxis.</p>
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Investigation of the physiological function of TTBK2 (tau tubulin kinase 2), a protein kinase mutated in spinocerebellar ataxia type 11 (SCA11)
… examined via mutagenesis and optical imaging of pHluorin-tagged proteins in cultured neurons from wild-type mice.<br/>
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Studies of plasticity after neuronal injury
… plasticity. By expressing the construct synaptopHluorin (SpH), lentiviral and adenoviral-associated viral vectors were used to label presynaptic terminals in vitro and in vivo. As the pHluorin component of the label is pH sensitive, this tool has the potential additional advantage of enabling …
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Synaptic vesicle recycling <i>in Vivo</i>
… wobei es sich einerseits um die Untersuchung von pHluorin Drosophila Larven in Kombination mit einer Injektion des Protonenpumpen-Inhibitors Bafilomycin und andererseits um die elektronenmikroskopische Untersuchung der Drosophila Mutante shibire handelte, welche ein temperaturabhängiges …
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Novel Optical Tools to Investigate Presenilin Control of Neurotransmission
… regulating neurotransmission. We utilized vGlut1-pHluorin, a pH sensitive analog of GFP tagged to a synaptic vesicle protein, that allows for reporting of synaptic vesicle cycling. These experiments found evidence that Presenilins do control neurotransmission and that they do so by impacting the …
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Developmental trajectory of synaptic vesicle recycling in wild type and neurodevelopmental disorder mqodels
… influx from 3 days in vitro (DIV). Synaptophysin-pHluorin (sypHy) imaging indicated that SVs are recycled via evoked exocytosis and endocytosis from the first week in vitro as well, although the number of responsive nerve terminals continued to increase until the second week in vitro. However, the …
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A Comparative Study of Sensory Neuron Synaptic Activity and the Role of Presynaptic Diversity, Specificity, and Regulation in Caenorhabitis Elegans
… neural circuitry of C. elegans. Here we use pHluorin imaging (Vglut-pH) to monitor presynaptic glutamate release from single chemosensory neurons in intact animals and characterize the dynamics of endo- and exocytosis from two types of glutamatergic neurons, AWC<sup>ON</sup> and ASH. In …