Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 181 for “"pH-dependent"”.
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A study of the pH-dependent charge of a selection of highly weathered humic soils
… from the development of positive charge at low pH in soils with variable charge which is enhanced by the amphoteric behaviour of Fe and AI oxides as well as the presence of organic matter. Aluminium tends to form stable complexes with organic matter and also competes with lower valency cations …
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Investigation towards controlled precipitation of nickel using H2S(g) by harnessing pH dependent sulphide speciation
Sulphide as a precipitating agent is effective as it facilitates the removal of heavy metals to very low residual concentrations (ppm - ppb levels) over a wide pH range, owing to the low solubilities (Ksp) of metal sulphides. However, previous work on metal sulphide precipitation has highlighted a …
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Construction of CNFY-CNF1 Chimera Protein for Study of Differential pH Dependent Activity of CNF Proteins
In this thesis we will explore the topic of cytotoxic necrotizing factors (CNFs) and show the creation of chimeric constructs to further test the details of CNF translocation into the cell. The CNF family of toxins is under the larger family of dermonecrotic AB toxins. These AB toxins have the …
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Novel In-Vitro Approaches to Investigate Membrane Partitioning and pH-Dependent Passive Permeation of Small Molecule Drugs
… bases or acids. For these drugs, along with lipophilicity and other physicochemical properties, the acid/base nature and ionization constant (pKa) are very important for predicting their passive permeation across biological barriers. Depending on the pKa of ionizable groups, drug molecules may …
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Expression of cysteine-rich pig gastric mucin (Muc5AC) domains in Pichia pastoris and their pH-dependent properties
… gel exhibiting a sol-gel transition at the low pHs found in the stomach. Our laboratory has demonstrated that the non-glycosylated cysteine-rich (Cys-rich) regions of the protein core of PGM play important roles in the hydrophobic, electrostatic and disulfide-bond interactions involved in PGM …
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The functional motifs for nucleocytoplasmic shuttling of C-terminal fragment of c-Met : A novel "pH-dependent' nuclear localization signal
… whose positive charge changes depending on pH, rather than the lysines or arginines, commonly found in classical bipartite NLSs, suggesting the possible ‘pH-dependency’ of this NLS. Indeed, decreasing the cytosolic pH either with nigericin, a Na+/H+ exchanger or low pH KRB (pH=6.5) buffer …
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Comparative analysis of cytotoxic necrotizing factor (CNF) toxins: Compatibility of cargo with delivery vehicle and identification of amino acid residues that modulate pH-dependent cytosolic cargo delivery
… in CNF3 promotes endosomal escape at a higher pH, leading to more efficient cytosolic delivery. These findings provide insight into the amino acid determinants of bacterial toxins that can be exploited to optimize cytosolic delivery of biologic cargos using BTIDD platforms.
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Interfacial Properties of Environmentally Responsive Polymers
… force measurements quantified the substantial pH-dependent change in the molecular properties of end-grafted NIPAM-N-Gly-(C18) 2 monolayers. At pH 8.0, where the glycine side chains are ionized, the polymers exhibit stereotypical polyelectrolyte behavior. Side chain neutralization at pH 5.0 …
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Computer simulation of titration behavior in proteins
The pH dependent properties of proteins can be successfully modeled using continuum electrostatic methods provided that a sufficiently detailed and accurate model of the protein system is used. In this work, the effects of conformational changes on calculated pK$\rm\sb{a}$ values in peptide and …
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Multilayer capsules with stimuli-sensitive properties : pH-response and carbohydrate-sensing
… Such capsules were prepared, with the focus on pH-sensitivity and carbohydrate-sensing. First, pH-responsive polyelectrolyte capsules were produced by means of electrostatic layer-by-layer assembly of oppositely charged weak polyelectrolytes onto colloidal templates that were subsequently …
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Antimicrobial activity of human seminal plasma and seminal plasma proteins
… The activity of these peptides was strictly zinc-dependent. We also found potent antifungal activity in seminal unleashed by the acid vaginal pH. The antifungal activity was mediated by beta-microseminoprotein (MSP). The activity of MSP mapped to a region in the C-terminal part of the protein. The …
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Determination of Calcium Binding to Beta-Casein at Various Ph Values Using the Calcium Murexide Method
… during processing. Since calcium binding is pH dependent, it is important to understand the binding properties of the non-micellar caseins with calcium ions under the influence of pH. The calcium murexide method which is based on the dissociation of calcium salt of murexide was found to be …
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Dissecting the Entry Pathways of Hepatitis C and Bovine Viral Diarrhea Viruses
… the mechanisms of superinfection exclusion, the phenomenon by which previous viral infection prevents reinfection of the same cell, and the pathways of entry into target cells for these viruses. BVDV-acutely infected cells establish two blocks to superinfection, at the levels of virus entry and …
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Applications for imidazophenanthridine-based heterocycles
The physical properties and reactivity of 2,3-dihydro-1H-imidazo[1,2-f]phenanthridinium cations (DIPs) and analogous heterocycles have been investigated. The adapted syntheses developed were applied to a range of aryl amines and more elaborate substrates, such as bifunctional aminoquinolinium …
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Solid-state nuclear magnetic resonance investigations of the influenza M2 protein : structure and dynamics characterization of channel gating and conduction
… bound to native-like lipid bilayers. Using pH-dependent 15N chemical shifts of the conserved Histidine residue, we have found that the proton conduction mechanism is the same in all of the M2 channels (WT AM2, S3 IN AM2, BM2) studied, and that the conduction cycle involves proton shuttling …
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NMR characterization of a diiron macrocycle and structural characterization of a diketo derivative
<p>The time-dependent visible spectra and the crystal structure of [Fe<sub>2</sub>(C<sub>20</sub>H<sub>24</sub>N<sub>8</sub>O<sub>2</sub>)(CH<sub>3</sub>CN)<sub>4</sub>]·PF<sub>6</sub> (diketo-dimer) were studied. The spectra showed that the most significant chemistry occurred during the initial …
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Investigating the Tyrosine-Histidine Linkage in the Copper B Site of Cytochrome C Oxidase: Model Studies Utilizing a Zinc Imidazole-Phenol Containing Complex
… incorporating an ortho substituted imidazole-phenol moiety. The resultant Zn(II) complex, [Zn(BPAIP)(Br)]+, was found to exhibit a decreased pK A (8.24) and increased oxidation potential relative to the unbound BPAIP. In addition, the stability of [Zn(BPAIP)(Br)]+ in buffered D2O was found to …
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Surface Charge Regulation Effects on Fluidic Nanoscale Systems
… to volume ratios of these systems, interfacial phenomena are of key importance to understanding their behavior. We present several studies of uidic nanoscale systems using the Poisson{Boltzmann equation which treats the uid as a continuum, and density functional theory of uids which uses a …
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The Measurement of an Environmentally Important Surfactant System Using Electrochemiluminescence
… and Ru(bpy)₃²⁺. The ECL emission is also pH dependent, with maximum intensities observed at pH 6-8. These results parallel those observed when tri-n-propylamine is substituted for EthomeenT/15 or T/25 and indicate a possible mechanism for ECL generation.
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