Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"pH regulation"”.
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Räumliche und zeitliche Aspekte der intrazellulären pH-Regulation in Epithelien
Die Speicheldrüsen der Schmeißfliege Calliphora vicina produzieren bei Stimulierung mit dem Neurohormon Serotonin (5-Hydroxytryptamine, 5-HT) einen KCl-reichen Primärspeichel. Der transepitheliale K+-Transport wird durch eine apikal lokalisierte vakuoläre H+-ATPase (V-ATPase) energetisiert. …
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Die pH-Regulation isoliert in vitro perfundierter Kolonkrypten der Ratte
… und die Homöostase ihres zytosolischen pH (pHi) mit Hilfe des pH-empfindlichen Fluoreszenzfarbstoffes BCECF untersucht. Die Perfusionstechnik erlaubte es, Transportprozesse eindeutig der luminalen oder basolateralen Membran zuzuordnen. Da im Dickdarm hohe Konzentrationen schwacher …
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Design, Fabrication, and Test of Chaotic Micromixers, Hydrogel Microvalves and pH Regulation Systems
… integrated microsystems designed to provide self regulation of pH in bioassays are demonstrated. The first system is based on the counterflow microdialysis principle, whereas the second system involves direct addition of an acidic or basic buffer solution into a sample solution. In both systems, a …
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Mechanisms of pH regulation and the generation of the electroretinogramb-wave in the toad retina
In the work presented in this thesis, several physiological properties of the isolated toad retina, including extracellular pH, light-evoked extracellular K$\sp{+}$ changes, light-evoked responses of rods and Muller cells, and the electroretinogram (ERG), have been studied under various conditions.
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Hv1 novel peptide inhibitor extracted from tarantula venom
… voltage-gated proton (Hv1) channels, critical in pH regulation and cellular signaling. Utilizing patch clamp techniques on genetically modified Chinese Hamster Ovary cells expressing human Hv1, the study revealed that GsAF-I significantly stabilizes the closed state of these channels, thereby …
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Engineering Hv1 Channel Mutations and Targeted Drug for Treatment of Hv1 Mutation Associated with Cancer
… channel Hv1 is crucial for maintaining cellular pH homeostasis by regulating proton movement across membranes, playing a vital role in acid extrusion and intracellular pH regulation. Dysregulation of Hv1 is associated with various diseases, including cancer, where it helps maintain an acidic …
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Novel Mechanisms of Antigen Processing That Enhance Bcg Vaccine Efficacy
… that BCG vaccine becomes sequestered in special phagosome compartments of macrophages that do not fuse with lysosomes. Since lysosome fusion is necessary for peptide production and T cell priming leading to protective T<sub>H</sub>1 immunity, we hypothesized that vaccine efficacy is reduced and …
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The production of 4-adminobutyrate in response to treatments reducing cytosolic pH and the regulation of intracellular pH
… H+ consumption are parts of a metabolic pH-stat mechanism which ameliorates the intracellular pH decline associated with anaerobiosis or other treatments. To test this hypothesis GABA production and efflux have been measured in isolated Asparagus sprengeri cells in response to three …
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Acid-base regulation in the sea urchin Parechinus angulosus during CO₂-induced seawater acidification
… is predicted to have adverse effects on the physiologies of marine organisms, particularly those that produce calcified structures. Extracellular homeostasis is considered to be critical to mediating the effects of ocean acidification. Due to their low metabolic rates and weak ability to …
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Protons and Potentials: How Neurotransmission Influences and is Influenced by pH Transients
… and can form transient increases or decreases in pH. These transients can modulate neuronal activity by altering neuronal excitability, either by inhibiting or potentiating ion channels or by acting on receptors. Changes in pH and their influence on activity are variable and context dependent, …
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The function of the hepatitis C virus p7 protein
… To evaluate the contribution of p7 to organelle pH regulation and virus production, a fluorescent pH sensor was incorporated within native, intracellular vesicles in the presence or absence of p7. p7 increased proton conductance in vesicles and was able to rapidly equilibrate H+ gradients. This …
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The Role of a Neuron-Specific V-ATPase in Synapse Specification, Function, and Maintenance
The Vacuolar-type (V-) adenosine triphosphatase (ATPase) is a proton-pumping nanomachine consisting of two multi-subunit, reversibly associating protein sectors, the cytosolic V1 sector and the membrane-bound Vo sector. The V1/Vo holoenzyme hydrolyses ATP to translocate protons across biological …
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Development of seawater electrolysis with low-cost membranes for sustainable cement production and pretreatment
… can be effectively facilitated through precise pH regulation. In conclusion, this thesis demonstrates the feasibility of UF membrane electrolysis as a low-cost, scalable solution for decarbonized cement production, with additional applications in seawater desalination and valuable resource …
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Targeting Sequence and Function-Dependence of Subcellular Localization of Transient Receptor Potential Mucolipin Channels and Characterization of Engineered Molecule Probes For Detecting Lysosomal Calcium Release
… such as divalent cation release, luminal pH regulation, autophagy and vesicle trafficking. Interestingly, whereas TRPML1 is almost extensively localized in lysosomes, TRPML3 is typically found in both lysosomes and the plasma membrane (PM). It has been shown that TRPML3 localization …
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Korrelation von in vivo-Resistenz gegen Chloroquin mit allelischen Polymorphismen bei Plasmodium falciparum-Isolaten aus Uganda
… Nebenwirkungen und niedriger Kosten bei der Prophylaxe und Behandlung der unkomplizierten Malaria jahrelang favorisiert worden war, wird durch zunehmende Resistenz des Erregers Plasmodium falciparum beeinträchtigt [Ridley 1998, Wellems & Plowe 2001]. Studien über die Wirkungsweise Chloroquins – …
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Synthesis and characterization of aminated perfluoro polymer electrolytes
… exclusion of a strong singlet around 2.9 ppm. pH indication tests with Thymolphthalein illuminated the slight presence of free OH- ions within the polymer following counterion exchange thus validating the low IEC and formation of a predominately zwitterionic polymer. Recommended future work …
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Modeling Nitrogen and Energy Metabolism in the Bovine
… in the model, 3) improve the representation of pH and to refit parameters related to ruminal metabolism and nutrient digestion in the model, 4) investigate how ruminal pH affects the microbial community, expression of carbohydrate-active enzyme transcripts (CAZymes), fiber degradation, and short …
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Structural and Functional Studies of SLO K+ Channels: Mechanisms of Gating by Intracellular Signaling
… in SLO1, Na<sup>+</sup> binding in SLO2 and pH increase in SLO3. It is known that intracellular signals activate SLO channels by acting on the large cytoplasmic domains (CTDs) of these proteins, which follows the transmembrane ionconduction pore. However, a molecular description of the …