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Showing 1 to 2 of 2 for “"pFGB 벡터"”.
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Analysis of Spacer Region derived from Recombination between Heterologous loxPs
… 재조합 반응으로 나타나는 spacer region의 염기 서열 분석을 위하여 새로운 벡터 pFGB를 제작하였다. 2272, M2, M3, M7, M11으로 총 25개의 pFGB-lox1/lox2 조합을 구성했다. Cre를 발현하는 BS1365 박테리아 내로 이 plasmid들을 형질전환하고 37℃에서 하룻밤 배양한 후, BS1365로부터 plasmid DNA를 분리하였다. 분리된 DNA를 JM109 박테리아에 형질전환하고 ampicillin, X-gal, IPTG를 포함하는 LB agar plate에 도말하여 배양하였다. 그 …
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Measurement of Efficiency of Cre-mediated Recombination between lox loxSites Using a New Vector System
… system. MATERIAL AND METHODS: A new vector (pFGB) to analyze Cre-mediated recombination efficiency between two lox sites was constructed. The combinations of wild type and mutant lox sites that are lox wt, M2, M3, M7, 2272, and 5171 were cloned into a pFGB vector, resulting in 36 pFGB-lox/lox …