Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"p85"”.
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Ubiquitination of EpoR and p85 in Ligand Induced EpoR Down-Regulation
… Epo induces Cbl-dependent ubiquitination of the p85, which binds to phosphotyrosines on EpoR. Ubiquitination allows p85 to interact with epsin-1, thereby driving EpoR endocytosis. Knockdown of Cbl, expression of its dominant negative forms, or expression of an epsin-1 mutant all compromise …
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Mechanistic Investigation of Genotype-Phenotype Correlations in PIK3R1-Related Diseases
The PIK3R1 gene encodes three proteins - p85$\alpha$, p50$\alpha$ and p55$\alpha$ - that are regulatory subunits of Class IA phosphoinositide 3-kinases (PI3Ks). These regulatory subunits heterodimerise with one of three catalytic subunit isoforms, namely p110$\alpha$, p110$\beta$, or p110$\delta$. …
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Characterising the effects of ACK-mediated phosphorylation on the Class IA PI3-Kinase regulatory isoforms
… of the Class IA PI3-kinase regulatory subunits (p85α, p85β, and p55γ). ACK has since been shown to bind to all five p85 isoforms and phosphorylate four (not p55γ) at a Tyr residue (equivalent to p85α Tyr607) in the inter-SH2 (iSH2) domain, a region situated between the N-terminal SH2 (nSH2) and …
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Einfluss des Insulin-ähnlichen Wachstumsfaktors I auf die Androgenrezeptor-Signaltransduktion in Prostatakrebszellen
… Bindung zwischen der C-SH2-Domäne der p85-Untereinheit der PI3-Kinase und dem N- und C-Terminus des Androgenrezeptors nachgewiesen werden. Durch die nähere Charakterisierung dieser Bindungsbereiche mit Hilfe von Peptidarrays und anschließenden Alanin-Substitutionen war es möglich, für …
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Nephrin und CD2AP aktivieren den PI3-Kinase/AKT-Signalweg im Podozyten
… Schlitzmembranproteine Nephrin und CD2AP mit p85, der regulatorischen Untereinheit der PI3-Kinase, interagieren. <br>Die Interaktion von Nephrin und p85 ist Phospho-Tyrosin-abhängig und kann durch Genistein, das die Phosphorylierung des intrazellulären Nephrin-Abschnittes hemmt, aufgehoben …
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The Functional Analysis of A Major Tyrosine Phosphorylation Site On Actin
… array, we identify N-terminal SH2 domains of p85, regulatory subunits of Phosphatidylinositol 3-kinase (PI3K), and VAV2, a Rho GTPase guanine nucleotide exchange factor, as phosphorylation-dependent binding partners of an actin pY53 peptide. Through biochemical and structural biology …
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Insulin Receptor Substrate-1 Serine Phosphorylation by a Novel Phosphatidylinositol-3'-Kinase-Associated Serine Kinase Regulates Insulin and Interferon Receptor Signaling
… serine phosphoprotein (pp76) distinct from the p85 subunit of PI 3-kinase. pp76 is phosphorylated by an okadaic acid sensitive, PI 3-kinase associated serine kinase (PAS kinase) with biochemical properties that distinguish it from the intrinsic serine kinase activity of PI 3-kinase and evidence …
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Dissection of CD2AP functions in epithelial cells
… showed that CD2AP co-localized with PI3K p85 while biochemical analysis showed that CD2AP binds directly to p85 in vitro. Depleting CD2AP in cells showed that it is necessary for localizing PI3K to cell-cell boundaries and for PIP3 accumulation along lateral membranes. CD2AP is also …
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Signal Regulation By Cd38, Lyn, And Mapk-Associated Molecules During All-Trans Retinoic Acid-Induced Differentiation Of Acute Myelogenous Leukemia Cells
… drive phosphorylation of ERK, c-Cbl, and p85 PI3K. We found that crippling enzymatic activity with inhibitors or a point mutation (CD38 E226Q) did not affect ATRA induction, while a cytosolic truncation (CD38 [DELTA]11-20) that prevented CD38 membrane expression crippled ATRA-induced …
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Assembly of nuclear dimers of the PI3K regulatory subunits underpins the proliferative activity of Activated Cdc42-associated Kinase, ACK
… directly phosphorylates 4 out of the 5 isoforms (p85α, p55α, p50α, p85β) on a conserved tyrosine located on the iSH2 region (Tyr607 for p85α). Phosphorylation of p85β at this site was found to promote cell proliferation, yet counterintuitively, ACK does not stimulate PI3K catalytic activity. This …
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Funktion der Protein-Tyrosin-Phosphatase SHP2 und des feedback-Inhibitors SOCS3 in der IL-6-Signaltransduktion
… nor with Gab1. Interestingly, the loss of the p85 recruitment sites on Gab1 was found to be critical for Erk activation in SHP2(Ex3-/-) cells. The catalytic activity of PI3K was shown not to be involved in IL-6 mediated Erk activation, so it is likely that either p85 is able to link gp130 and …
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Regulation of Exercise-Dependent Cardiac Growth by MicroRNAs
… repression of a network of genes including p85-alpha, Rictor, Lin7c and Fzd4. Collectively, the results of my thesis research identify miR-499 as a pivotal regulator of exercise-induced cardiac hypertrophy.
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Investigating PLEKHS1 adaptor function in PI3K signalling and human cancer biology
… with endogenous PI3K regulatory subunits (p85, p55γ). PLEKHS1 phosphorylation and PI3K interaction are driven by SFK activity and independent of serum growth factors. In this context and alike *in vivo* findings, the PLEKHS1 PH domain which specifically binds PIP<sub>3</sub> and …
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Polymeric Complexes and Composites for Aerospace and Biomedical Applications
… composites. In chapter 4, a novel Pluronic® P85-bearing penta-block copolymer was synthesized and formed complexes with magnetite. The complexes displayed non-toxicity to cells normally but were able to selectively kill cancer cells without killing normal cells when subjected to a …
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Einfluss von Tensiden auf die Resorption von Substraten der ABC-Transportproteine
… durch p-gp, wobei die Poloxamere, z.B. Pluronic P85, die größte Wirkung zeigten. Einige Tenside hemmten den Transport durch MRP2. Hierbei waren Cremophor RH40 und Solutol HS15 am effektivsten. Nach Bestimmung der Kritischen Mizellbildungskonzentration zeigte sich, dass die effektivsten …
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Factors Regulating the Intrarenal Renin-Angiotensin System and Renal Damage During Aging
… and an increase in the expression of the PI3K p85 regulatory subunit. L-158,809 increased angiotensinogen, ACE, ACE2 and neprilysin mRNA with a similar trend for renin. RAS blockade also increased AT1b, AT2, Mas and leptin receptor mRNA as well as the p85 subunit expression. Thus, insulin …
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Pathophysiologie des Hörschadens bei experimenteller Pneumokokkenmeninigitis
… neuronale Nekrose (Histologie, TUNEL, PARP p85-Spaltprodukt Immunhistochemie) mündete in eine verminderte Neuronendichte im Ganglion spirale. Dieser Neuronenverlust war zwei Wochen nach Infektion neben einer fibrozytären Okklusion der perilymphatischen Räume (beginnende Labyrinthitis …
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Molecular Mechanisms Involved in Escherichia coli K1 and Haemophilus influenzae Type a Blood-Brain Barrier Impairment
… Growth Factor Receptor 1 (VEGFR-1) and p85 subunit of PI3K. All these events lead to increase of BBB permeability and bacterial invasion of microvascular endothelial cells. It is still unknown if the emergency of invasive Hia disease is due to the serotype replacement after the wide use …
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Effects of Zinc on the Insulin Receptor and Insulin Signal Transduction Pathway
… of IRS-1 and -2, and the association of the p85 subunit of PI 3-kinase and IRS-1. Zinc significantly increased a marker of Akt activation, serine 473 phosphorylation of Akt, which was totally blocked by wortmannin. It appears that zinc induces an increase in glucose transport into cells and …
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Approaches towards therapeutic development against chronic brucellosis in a mouse model
… The non-ionic block co-polymer Pluronic P85, when mixed with OMVs as an adjuvant showed significantly higher protection against B. melitensis challenge in vaccinated mice compared to those vaccinated with OMVs alone. When tested as immunostimulants, there was no additive effect of …
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