Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 57 for “"p62"”.
-
THE AUTOPHAGIC RECEPTOR P62 MODULATES MICRONUCLEUS STABILITY
… Notably, I found that the autophagic receptor p62 recognizes micronuclei. p62 localizes to micronuclei spontaneously forming in colorectal cancer cell lines as well as those generated upon induction of chromosome mis-segregation in both untransformed and transformed cell lines. p62 can access …
-
The Discovery of p62-dependent Processing Bodies
… <br/>selective autophagy receptor Sequestome 1 (p62) undergoes liquid-liquid phase separation <br/>(LLPS) to form p62 bodies to mediate the autophagic degradation of ubiquitinated proteins. <br/>At the time of this research, it was unknown whether p62 could exist in a differential droplet …
-
The study of two transmembrane autophagy proteins and the autophagy receptor, p62
… for the most common autophagy receptor protein, p62. I show that p62 can specifically associate with and sequester LC3-I in autophagy-impaired cells (ATG9 and ATG16 null cells) leading to formation of LC3-positive structures that can be misinterpreted as mature autophagosomes. Perturbations in …
-
The role of DAXX in the selective autophagy receptor SQSTM1/p62 phase condensation
… which is dependent on cargo receptors. SQSTM1/p62 (‘p62’ hereafter) is one of the best characterised receptors mediating the autophagic degradation of misfolded proteins, and its body formation is essential for its cargo recognition and clearance. Mounting evidence has revealed that …
-
FROM MICRONUCLEI TO EMT: MOLECULAR MECHANISMS OF GENOMIC INSTABILITY AND CELLULAR PLASTICITY
… investigates the role of the autophagic receptor p62 in regulating micronuclear integrity. Utilizing advanced methodologies, including proximity labeling and mass spectrometry, this study reveals how p62 recognizes micronuclei through ubiquitination and proximity to mitochondria, with ROS-induced …
-
LNX1 and LNX2 in the central nervous system: linking behavioural, cellular and molecular perspectives
… function. In the central nervous system, LNX1p70/p62 isoforms and LNX2 show dynamic expression during development, with nearly undetectable protein abundance in the adult brain, making them notoriously difficult to study. Despite their low abundance, both proteins interact with multiple synaptic …
-
The Nuclear Pore Complex: Novel Glycoprotein Constituents
… that recognizes predominantly a 62 kD protein (p62) of rat liver nuclei. This protein remains associated with the nuclear pore complex-lamina fraction that results from the treatment of nuclei with DNase, RNase, and nonionic detergent. Immunofluorescence microscopy revealed a strikingly punctate …
-
Differential regulation of the phosphorylated and dephosphorylated forms of the ubiquitin-conjugating enzyme Ubc6e
… demonstrated that Ubc6e is co-localised with p62/sequestrosome, which is a cargo receptor that delivers ubiquitinated cargo to autophagosomes for degradation. To further investigate the possible role that Ubc6e may play in autophagy, we aimed to investigate any protein-protein interactions …
-
Identification of Cathepsin B and L as Novel Uva Targets Upstream of Cutaneous Lysosomal-Autophagic Dysregulation
… of cellular protein levels of LC3-II and p62 (sequestosome 1) in UVA-exposed fibroblasts. Furthermore, UVA-exposure modulated transcriptional levels of p62 (sequestosome 1, SQSTM1), α-synuclein (SNCA), and transglutaminase-2 (TGM2). Strikingly, pharmacological cathepsin B/L inhibition …
-
Dual Roles for NBR1 in Bone Remodelling and Autophagosomal Protein Degradation
… interacts with the structurally similar protein p62. The aims of this thesis were to further characterise the biological functions of Nbrl by identifying novel protein interactors. A yeast-2-hybrid screen revealed a direct interaction between Nbrl and the autophagic effector protein LC3. This …
-
Characterising the role of Valosin Containing Protein (VCP) in autophagy and cell differentiation.
… I have shown that VCP co-localises with p62 and LC3 at the subcellular level in cells undergoing autophagy and that VCP co-immunoprecipitates with p62 in the autophagy-dependant manner. I have also examined the stability of VCP in the cell and shown that p62 has a role in stabilising the …
-
Analysis of markers of cell division cycle, apoptosis and autophagy flux in glioblastoma
… form part of the core autophagy machinery; and p62, a mammalian autophagy receptor that binds ubiquitinated proteins. A total of 66 patients were recruited to the study between 2007 and 2009. Data were collected on patient demographics, pre-operative Karnofsky score, surgical and adjuvant …
-
The intrinsic role of SOCS1 in intestinal epithelial cells
… (Lg5, E-cadherin, Muc2 and Tff3), autophagy (P62), ER stress response (spliced Xbp1) and apoptosis (cleaved caspase 3 protein), as well as EdU incorporation proliferation assays were then performed. Our results indicate that the loss of Ifng and Socs1 is not affecting colonoid growth and …
-
Μελέτη του ρόλου των εξωσωματίων των μεσεγχυματικών στρωματικών κυττάρων στην οξεία ηπατική ανεπάρκεια
… αυτοφαγοσωμάτων) και τα επίπεδα της πρωτεΐνης p62 (sequestosome 1), η οποία συσσωρεύεται σε περιπτώσεις παρεμποδισμένης αυτοφαγίας, αξιολογήθηκε η λειτουργικότητα του μηχανισμού υπό συνθήκες ηπατικής βλάβης. Τα πειραματικά μας δεδομένα κατέδειξαν ότι η χορήγηση της MFGE-8 δρα καταλυτικά στην …
-
The role of USP17 in lysosome trafficking and lysosomal function
… lysosome positioning via the ubiquitination of p62 to tether vesicles to the ER, and in particular USP17 is shown to deubiquitinate p62.<br/><br/>Overall, the data illustrates that USP17 is important for lysosome trafficking and lysosomal function. These findings provide us with more …
-
Characterisation of phenotypes of inflammation, fibrosis and remodeling in chronic rheumatic heart disease using multiparametric cardiovascular magnetic resonance and autophagy markers
… we analysed the immunoexpression of Beclin, LC3, p62, BAX, Bcl-2 and caspase-3 in patients confirmed with RHD and valvular heart disease
-
A ROLE OF PRL-3 IN AUTOPHAGY AND ITS REGULATION IN HUMAN CANCERS
… ALSO ACCELERATES THE DEGRADATION OF SQSTM/P62, WHICH IS A KNOWN AUTOPHAGY SUBSTRATE. SURPRISINGLY, PRL-3 ITSELF IS ALSO DEGRADED BY AUTOPHAGY, FORMING A NEGATIVE FEEDBACK LOOP WITH AUTOPHAGY. CLINICALLY, PRL-3 IS DEPENDENT ON AUTOPHAGY ACTIVITY IN PROMOTING OVARIAN CANCER PROGRESSION. IN …
-
Non-resolving pro-inflammatory macrophage polarization by super-low doses of bacterial endotoxin
… novel upregulation of pro-inflammatory mediators p62 and ccl2 with simultaneous downregulation of homeostatic mediators Nrf2 and slc40a1 in immortalized wild-type mouse macrophages. Further we showed this effect was reversed using the homeostatic restorative agent sodium phenylbutyrate (4-PBA), a …
-
CROSS-DISEASE ANALYSIS OF MOLECULAR AND CELLULAR MECHANISMS IN KIF5A-ASSOCIATED NEURODEGENERATIVE DISORDERS
… KIF5A mutant also formed sequestosome 1/p62 (SQSTM1/p62)-positive detergent-insoluble inclusions sequestering WT KIF5A, indicating a gain of toxic function. Cycloheximide chase assays in SH-SY5Y neuroblastoma cells demonstrated a shorter half-life for the SPG10-related R17Q and …
-
Corneal fibrosis therapies and investigations in the canine corna in vitro
… of autophagy-related genes (LC3, Beclin1, Sqstm1/p62, and Lamp1) in healthy and injured corneal stromal fibroblasts. Methods: Primary cultures of corneal stromal fibroblasts (CSFs) were generated from healthy donor canine corneas and grown in minimum essential medium (MEM) containing 10 percent …
Page 1 of 3