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Showing 1 to 20 of 387 for “"p450"”.
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P450 biochips : development of a protein microarray platform for investigating cytochrome P450 clinical drug metabolism
… describes the development of a novel cytochrome P450 array format, the P450 Biochip that allows quantitative and truly high-throughput measurement of cytochrome P450-mediated turnover reactions in sub-nanolitre volumes.
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Characterization of Monomeric Human Cytochrome P450 3A4 and Cytochrome P450 Reductase in Nanoscale Phospholipid Bilayer Discs
… or CYP3A4 and its redox partner, cytochrome P450 reductase, as a coincorporated complex are described and further characterized for substrate binding, rates of dithionite dependent reduction, NADPH utilization rates, and testosterone metabolic rates as a function of substrate concentration. …
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A Thermostable Cytochrome P450 From Sulfolobus Solfataricus
… in the active site that are important in the P450 dioxygen scission reaction. CYP119 is found to display a mobile F-G helix and loop region that undergoes a significant conformational change upon binding of medium and large ligands and may reflect a natural motion independent of ligand …
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Catalytic site mutagenesis of cytochrome P450 6B1v1
Cytochromes P-450 form a large superfamily of heme-containing monooxygenases that are found in species ranging from bacteria to insects, plants, and mammals. In insects, P-450s have several functional roles, including growth, development, feeding, resistance to pesticides, and tolerance to plant …
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Cytochrome P450 whole cell biohydroxylation of alkanes
… to produce value added chemicals. Cytochrome P450 (CYP) monooxygenases such as CYP153A6 have previously shown excellent 95% selectivity for hydroxylation at the terminal carbon on n-octane and CYP153A13 has shown promising activity on n-octane and n-decane. In the present study, whole cell …
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Genetically engineering a new system for the expression of cytochrome P450 enzymes in insect cells using novel P450 reductases
Human cytochrome P450 enzymes (CYPs) are a superfamily of haem-binding monooxygenase enzymes involved in the metabolism of xenobiotics such as toxins, carcinogens and pharmaceutical drugs as well as in the biosynthesis of cholesterol, vitamins and steroids. In recent years, the area of drug …
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Moessbauer Studies of Cytochrome-P450 From Pseudomonas Putida
Made available in DSpace on 2015-05-12T22:39:07Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7412185.PDF: 4019834 bytes, checksum: e4e5fb11b5fca0d3a3f6ddfc3371bfcb (MD5) Previous issue date: 1973
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Functional effects of cytochrome P450 variants on drug metabolism and adverse drug reactions: developing and extending high throughput P450 protein technology platforms
Cytochrome P450 (CYPs) are a superfamily of heme containing enzymes that catalyse a diverse range of biological reactions. They are responsible for over 80% of primary metabolism of currently available drugs and are therefore central to its medical importance. Investigating the effects of these …
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In search of human cytochrome P450 1A1 enzyme inhibitor
Human cytochrome P450 1 (CYP1) family consists of three members; they are CYP1A1, CYP1A2 and CYP1B1. The mRNAs of CYP1A1 and CYP1B1 genes are known to be expressed in extra-hepatic tissues while that of the CYP1A2 gene is found in the liver amongst other tissues. Under normal circumstances, rarely …
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Global deconvolution of heterotropic cooperativity in cytochrome P450 3A4
Cytochrome P450 3A4 (CYP3A4) plays a central role in xenobiotic metabolism, and is of critical importance to both human health and the pharmaceutical industry. Its ability to interact with multiple molecules of the same substrate, or multiple substrates, leads to complex non-Michaelis kinetics, …
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Macromolecular recognition in the cytochrome P450(cam) enzyme system
The cytochrome P-450$\sb{\rm cam}$ reaction cycle is a complex set of coordinated chemical transformations requiring precise temporal and spatial control of reactivities. Cytochrome P-450$\sb{\rm cam}$ catalyzes the regio- and stereo-specific hydroxylation of camphor to form 5-exo-hydroxycamphor. …
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Zytochrom P450 in Gehirn und Leber der Maus (Mus Musculus) Über die Funktion von P450 im Testosteronmetabolismus und die Beeinflussung durch das Antiepileptikum Phenytoin
Viele Zytochrom P450-Isoformen sind durch spezifische exogene Substanzen, wie das Antiepileptikum Phenytoin, induzierbar. Einige dieser Enzyme sind am Metabolismus gonadaler Hormone beteiligt, welche eine wichtige Rolle bei Hirnentwicklung, -funktion und neuronaler Plastizität spielen. Allerdings …
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BIOACTIVATION OF NATURAL DIETARY PRODRUGS BY SPECIFIC CYTOCHROME P450 ENZYMES
Tumour specific cytochrome P450 enzymes (CYPs) have recently been established as a novel target in anticancer drug discovery. The enzymes are used to activate nontoxic prodrugs with latent activity. Thus, the combination of tumour specific CYPs as ‘rescue enzymes’ with a suitable substrate provides …
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Mechanistic Studies of Two Steroidogenic Cytochromes P450, CYP17A1 and CYP21A2
Human CYP17A1 (P450c17 or 17alpha-hydroxylase/17,20-lyase) is a member of the P450 superfamily of proteins. This enzyme is responsible for hydroxylating the 17-position of pregnenolone or progesterone and cleaving the 17,20-carbon,carbon-bond of its hydroxylated products, which leads to the …
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Characterisation of mammalian cytochrome P450 enzymes by their metabolic activities.
… characterise human liver microsomal cytochromes P450 responsible for the in vitro transformation of the B-carbolines, harmine and harman. Comparative studies were also carried out with rat liver microsomal P450s, where the effects of induction on the metabolism of harmine were investigated to …
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Cytochrome P450 mRNA profile in human breast cancer cell lines.
Cytochrome P450 enzymes (P450s) are involved in cancer development and treatment due to their roles in the oxidative metabolism of various compounds, both endogenous (e.g. oestrogen) and exogenous (e.g. tamoxifen). Intermediate P450 metabolites derived from oestrogen metabolism are associated with …
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Effects of Pycnogenol on Microsomal Cytochrome P450 Activity and Apoptosis
… activation of NNK depends upon cytochrome P450 enzymes (GYP). A second study looked at O-dealkylation of the substrates benzyloxyresorufin (BROD), ethoxyresorufin (EROD) and methoxyresorufin (MROD) linked to CYP2B1,2C6 and 1A2, respectively. Pycnogenol significantly inhibited BROD, EROD and …
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Investigating the evolution of cytochromes P450 involved in GA biosynthesis
… (KO), which are multifunctional cytochromes P450 that catalyze oxidation at the C4α methyl, converting ent-kaurene to ent -kaurenoic acid, an early step in gibberellin phytohormone biosynthesis. Arabidopsis produces no labdane-related diterpenoids other than gibberellins, whereas rice …
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