Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 75 for “"p27"”.
-
P27 Expression in Endothelin-1 Stimulated Mitogenesis
… through the cyclin dependent kinase inhibitor p27. BCEC were isolated from bovine eyes and grown in DMEM-10% serum in a 5% C02, 37° C incubator. Confluent cultures of BCEC were incubated for 24 hours with lOOnM Endothelin-1, luM BQ123 and BQ123 +100nM Endothelin-1 and some left untreated. Total …
-
The role of p27 phosphorylation in mediating atRA sensitivity of ovarian carcinoma cells
… (Cdks), and cyclin dependent kinase inhibitors. P27, a cyclin dependent kinase inhibitor regulating G1 checkpoint has been shown to have elevated levels in response to atRA treatment. In these studies we investigated the role of p27 phosphorylation in mediating atRA induced growth inhibition. Our …
-
Developing Constrained p27 Peptides to Target the Oncogenic E3 Ubiquitin Ligase SCF-Skp2
… is the cyclin-dependent kinase inhibitor p27 which positively regulates cell cycle progression. Elevated levels of Skp2 and reduced levels of p27 are common in a variety of cancers, including lymphomas and breast and prostate carcinomas. A lack of suitable binding pockets in Skp2 and the …
-
Posttranscriptional gene silencing of p27 in primary prostate epithelial cells by small inhibitory RNA (siRNA)
… regulated by Cdk-inhibitors such as the p21 and p27 Cip/Kip proteins. Defects in many of the molecules that regulate the cell cycle have been implicated in cancer development. An important example is the common downregulation of the Cdk inhibitor p27 in prostate cancers. p27/Kip1 regulates the …
-
The Effect of Endothelin-1 on the expression of CDK Inhibitors p21 & p27 in Bovine Corneal Endothelial Cells
… cyclin dependant kinase inhibitors (p21cip1 and p27kip1) levels by Endothelin-1 (ET-1), would overcome the G1 phase arrest and promote cell cycle progression and proliferation in cultured BCECs (Bovine corneal endothelial cells). BCECs were isolated from bovine corneas and cultured in DMEM …
-
A study to investigate the role of P27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinoma
Cyclin E and p27 expression is easy to assess in human tissues by standard immunohistochemical techniques. Immunohistochemistry is cost effective, relatively easy to perform and will play more of a role in the future management of cancer. To investigate the role of p27 and cyclin E immunoexpression …
-
Probing the Role of Nascent Helicity in Protein Function: p27kip1 as a Regulator of the Cell Cycle
<p>p27<sup>kip1</sup> binds to and regulates the activity of cyclin-dependent kinases (Cdks) which are the master timekeepers of the cell division cycle. Members of the p27 family of proteins, also including p21 and p57, are called cyclin-dependent kinase inhibitors (CKIs). The amino terminal …
-
Μελέτη ρυθμιστών του κυτταρικού κύκλου και παραγόντων που εμπλέκονται στη διεργασία αποδόμησης των P21cip1 και P27kip1 σε λεμφώματα Β-κυτταρικής αρχής. Συσχέτιση με κλινικές παραμέτρους
… αναστολέων του κυτταρικού κύκλου, p27 και p21, έχει δειχθεί να αυξάνει κατά την καρκινογένεση. Αν και η πρωτεόλυση των p27 και p21 έχει δειχθεί να μεσολαβείται από διαφορετικά μονοπάτια ωστόσο το περισσότερο γνωστό και καλύτερα τεκμηριωμένο είναι το μονοπάτι μέσω του συμπλόκου SCF …
-
Artemis Interacts With The Cul4A Ubiquitin E3 Ligase Complex and Regulates The Cell Cycle Progression
… Additionally, Artemis also interacts with p27, a substrate of Cul4A-DDB1 complex, and both DDB2 and Artemis are required for the degradation of p27 mediated by this complex. Furthermore, I show that the regulation of p27 by Artemis and DDB2 is critical for cell cycle progression in normally …
-
Role of Cyclin-Dependent Kinase Inhibitors in Estrogen Regulation of Adipogenesis
… on the levels of cell cycle inhibitors like p27 Kip1 (p27) and p21 Cip1 (p21) in breast cancer cell lines, estrogen might have an effect on the number of adipocytes by its effect on these proteins. Lack of p27 and p21 caused obesity due to hyperplasia in mice, which led to metabolic …
-
Discovery and Effects of Pharmacological Inhibition of The E3 Ligase Skp2 By Small Molecule Protein-Protein Interaction Disruptors
… are the cyclin-dependent kinase inhibitor, p27. Skp2 is an oncogene and studies have shown that over-expression of Skp2 leads to increased degradation of p27 and increased proliferation in several tumor types. Additionally, Skp2 is over-expressed in multiple human cancers. Clearly, Skp2 …
-
Substrate specificity and properties of heat-stable proteases from Pseudomonas fluorescens
… and lipases from strains P26, 32A, Pl, and P27 of Pseudomonas fluorescens were produced using a dialysis membrane method. The proteases were partially purified by molecular exclusion chromatography (MWC0=60,000) and selected biochemical characteristics were determined. Proteases of all …
-
Characterization of the molecular mechanisms underlying retinoic acid induced growth inhibition in MCF-7 human breast cancer cells.
… of the known cyclin-cdk inhibitors p21 and p27. As well, no change in the activity of the cdk activating kinase, CAK, was detected. These results suggest that the growth inhibition of MCF-7 cells is associated with a down regulation of cdk2 activity. While this reduced activity occurred in …
-
Identification of Novel Regulators in Hematopoiesis: Roles for Gfer in Hematopoietic Stem Cell Proliferation and CaMKK2 in the Restriction of Granulopoiesis
… of the cyclin-dependent kinase inhibitor (CDKI) p27<sup>kip1</sup>. In contrast, ectopic over-expression of Gfer in HSCs resulted in significantly elevated total and nuclear p27<sup>kip1</sup>. We next performed immunoprecipitation-immunoblot analyses to determine whether alteration of Gfer …
-
Notch signaling and cell cycle inhibitor regulation in pituitary organogenesis
… in increased expression of the CDKIs p21, p27 and p57. We hypothesize that this aberrant increase in expression may be a molecular mechanism contributing to progenitor pool depletion. Increased expression of CDKIs has been shown to be a hallmark of differentiating cells and loss of CDKIs …
-
Characterization of the Key Mouse Cochlear Developmental Genes for Auditory Hair Cell Regeneration
… proliferation of SCs by acute ablation of p27 or Sox2. Secondly, we overactivated Notch1 signaling in the mouse inner ear at different developmental stages, and found that the ability of Notch signaling in generating new HCs declines after birth. Last, we achieved reprogramming of neonatal …
-
Dissecting the molecular role of distinct binding interfaces on the retinoblastoma tumor suppressor in growth control and tumorigenesis.
… In addition, pRB can stabilize the CDK inhibitor p27 through an interaction with two ubiquitin ligase complexes. Further, pRB is capable of forming a unique interaction with E2F1 termed the ‘specific’ interaction that is capable of blocking E2F1 induced apoptosis. These functions of pRB are …
-
Structure Activity Relationships for Intracellular Loop 2 of the 5HT1A Serotonin Receptor
… and the current study includes peptides P21 to P27 of ic2 (LDRYWAITDPIDYVNKRTPRPR), approaching the ic2 carboxy terminus of TMD4 two residues per 10 MER. Peptide ability to uncouple receptor from G protein was determined in agonist inhibition studies using the selective 5HT1aR agonist …
-
Structure Activity Relationships for Intracellular Loop 2 of the 5HT1A Serotonin Receptor
… and the current study includes peptides P21 to P27 of ic2 (LDRYWAITDPIDYVNKRTPRPR), approaching the ic2 carboxy terminus of TMD4 two residues per 10 MER. Peptide ability to uncouple receptor from G protein was determined in agonist inhibition studies using the selective 5HT1aR agonist …
-
A Novel Drug Delivery System Incorporates a Functional Composite Scaffold Derived from Taxifolin with Potential Cytotoxic and Anticancer Effects on Triple Negative Breast Cancer
… as evidenced by changes in genes (p53, p21, p27,and Bcl-2) using qPCR and protein levels (p53, p27, and Bcl-2) using Western Blot, suggesting its potential in targeted therapy. The optimised taxifolin-PCL electrospun scaffold enhances cell adhesion, reducing cell viability and proliferation …
Page 1 of 4