Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"p16INK4a"”.
-
Creg1 And Its Enhancement Of P16ink4a-Induced Senescence
… a role in cellular senescence involving the p16INK4a-Rb pathway. Ectopic expression of CREG1 in the immortal LFS cell lines decreases cell proliferation but does not directly induce senescence. We confirmed this in osteosarcoma and fibrosarcoma cancer cell lines, similar to those seen in …
-
Study of Rest As A Negative Regulator of P16Ink4A
<p><strong>STUDY OF REST AS A NEGATIVE REGULATOR OF P16<sup>INK4A</sup></strong><strong> </strong></p> <p><strong>Monica Gireud, B.S.</strong></p> <p><strong>Thesis Advisor: Vidya Gopalakrishnan, Ph.D.</strong></p> <p>The<em> RE1</em> Silencing Transcription Factor (REST) is a negative regulator of …
-
Developing nanobodies to stabilise the tumour suppressor protein p16INK4a
The tumour suppressor protein p16INK4a (p16) is a cyclin-dependent kinase (CDK) inhibitor that plays a key role in the regulation of the cell cycle by controlling the progression of cells through the G1 to S phase transition. Dysregulation of the protein through deletion, silencing or mutation of …
-
Molecular changes involved in oral cancer progression and their relevance to keratinocyte immortalisation
… receptor (RAR)-b and the cell cycle inhibitor, p16INK4a, p53 mutations and increased levels of telomerase/hTERT mRNA. In contrast, increased expression of the EGF receptor (EGF-R), known to be a characteristic of oral cancer, does not occur until after the dysplasia stage in squamous cell …
-
Analysis of driver gene mutations in oesophageal squamous cell carcinoma
… knockdown experiments targeting p14ARF and p16INK4a in OSCC. In silico structural analysis of missense mutations in p14ARF and p16INK4a was conducted using UCSF Chimera tool. WGS analysis identified 35 frequently mutated genes in OSCC, among these, TP53, CDKN2A.p16INK4a, CDKN2A.p14ARF, and …
-
Early Blood Cell Formation "in sickness and health, ´till death do us part"
… was suggested to be decreased through increased p16INK4A signaling. To determine regulating factors underlying these observations, we assayed HSC in aged mice (Article II). We found evidence for decreased proliferative kinetics of old versus young HSC, though without proof of replicative …
-
Risk factors and diagnostic methods of high grade cervical squamous intraepithelial lesions /
… of fluorescence spectroscopy and microarray with p16INK4a marking in HSIL diagnostics were employed. Also, the diagnostic parameters of supernatant autofluorescence excitation and emission were established: Excitation of supernatant sample with 355 nm wave, emission 405–424 nm. The mobile micro …
-
A maternal high fat diet represses the expression of antioxidant defense genes and induces the cellular senescence pathway in the liver of male offspring rats
… of HF/C pups than in C/C pups. The expression of p16INK4a, a marker of cellular senescence, and cyclooxygenase-2 (Cox2), a pro-inflammatory marker, was significantly higher in the HF/C offspring group than in the C/C offspring group. Western blot analysis shows that cyclin D1 and phosphorylated …
-
Molecular Mechanisms in Hematopoietic Stem Cell Aging
… a decreased proliferation that depends on p16Ink4a activity. Although we observed (article I) a decreased replicative activity in physiologically aged HSC we found no evidence for increased p16Ink4a activity in these cells. In article II we demonstrated several hematopoietic defects …
-
Evaluation of novel prognostic factors In ovarian carcinoma
… examined to identify gene abnormalities in p53, p16INK4A, RB-1, p21WAF1/CIP1, Cyclin D1, Erb-B2, and MSH2. Adverse outcome was also examined in addition to survival and RFS, to identify novel molecular prognostic markers. P53 overexpression in 44 of 112 (39%) was associated with reduced survival …
-
Characterizing Human Papillomavirus Associated Oral Epithelial Dysplasia
… for histopathological features of HPV and p16INK4a immunoreactivity. Transcriptionally active infections were confirmed by performing RT-qPCR to identify E6 mRNA expression. We identified 82 cases of confirmed HPV-OED that showed a strong predilection for male patients and anatomical sites …
-
E2F1 and Tumor Suppression: The Role of P21, Mirnas, and The Dna Damage Response
… often deregulated through aberrations in the Rb-p16INK4a-cyclin D1 pathway. In these studies we examined three mechanisms by which E2F1 might mediate its tumor suppressive properties: p21-induced senescence, miRNAs, and the DNA damage response. We found that E2F1 acts as a tumor suppressor in …
-
PTEN regulates the BMI1-mediated self-renewal of long-term hematopoietic stem cells in developmental and stress conditions
… Consistently, the stem cell exhaustion marker p16Ink4a, which is typically repressed by BMI1, was strikingly upregulated in vCBPΔ/Δ LT-HSCs. These observations indicate that the protein levels of BMI1 are critical for the expression of p16Ink4a and the BMI1-mediated self-renewal regulation. At …
-
The ageing hair follicle environment. Alterations in the female scalp and mesenchyme with age.
… 1 (TIMP1), cyclin-dependent kinase inhibitor 2A (p16INK4A), 11β-Hydroxysteroid dehydrogenase type 1 and 2 (11β-HSD1/HSD11B1 and 11β-HSD2/HSD11B2) mRNA expression increased in aged DFs. In DS cells, HAS2, Vimentin (VIM) ,PDPN, Lysosomal-associated membrane protein 1 (LAMP1), Sequestosome- 1 (P62) …
-
The Role of Polycomb Repressive Complex 2 in Epidermal Homeostasis and Hair Growth
… upregulation of senescence-associated genes, p16INK4A and p19INK4D, suggesting EZH2 involvement in epidermal aging. In human anagen hair follicle (HF), EZH2 was detected in stem and progenitor cells; and hair matrix keratinocytes. Silencing EZH2 in HFs accelerated anagen-catagen transition and …
-
The direct effects of estradiol and several xenoestrogens on cell numbers of early- vs. late-stage prostate cancer cells
… of p38, apoptosis, and phosphorylation of p16INK4A also contributed. Our rapid effects suggested the participation of membrane ERs (mERs). ERα and β mediated E2’s ability to increase ROS through sustained ERK activation, and increased p-cyclin D1 levels in LAPC-4 cells. However, ERβ and …
-
Roles of CCN5 in regulating progression and therapeutic sensitivity of breast cancer
… indicate that CCN5 induces expression of P16INK4A and P19ARF, resulting in cell cycle arrest of the tumor cells. Collectively, these studies suggest that CCN5 restoration can be beneficial for the better management of breast cancer progression.
-
Analysis of senescence-like growth arrest induced by RUNX1 and its fusion derived oncoproteins
… RUNX1 drives senescence in cells lacking intact p16Ink4a. RUNX1-ETO appears to exert its unique effects through potent induction of reactive oxygen species and p38MAPK phosphorylation. These findings illustrate the heterogeneous manifestations of senescence-like growth arrest and elucidate the …