Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"oxysterol"”.
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The Signaling Pathway of Oxysterol-Induced Apoptosis in Macrophages.
… events associated with atherosclerosis. Oxysterols, the major cytotoxic component of OxLDL, induce apoptosis in macrophages by a calcium flux mediated activation of cytosolic phospholipase A<sub>2</sub> resulting in the release of arachidonic acid (AA). Inhibition of AA metabolism has …
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An Examination of Oxysterol Effects on Membrane Bilayers Using Molecular Dynamics Simulations
… I focus on a group of small molecules called oxysterols, oxygenated derivatives of cholesterol. They are present in small concentrations in mammalian cells and have been shown to be involved in multiple regulatory pathways involving control of cellular cholesterol levels. The mechanisms …
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The Signaling Pathway of Oxysterol Induced Apoptosis in Chinese Hamster Ovary (CHO)-K1 Cells.
… ovary (CHO)-K1 cells treated with a potent oxysterol, 25-hydroxycholesterol (25-OHC). In the present study, we find that oxLDL can induce apoptosis in any cell types if cells present the specific receptors on their surface to take up oxLDL, and that apoptosis-inducing activity is associated …
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Oxysterols in Neurodegeneration and the Influence of Presynaptic α-Synuclein Phosphorylation
… of oxidised cholesterol derivatives known as oxysterols. Notably, 24S-hydroxycholesterol (24S-HC) and 27-hydroxycholesterol (27-HC) have been implicated in exacerbating neurodegenerative processes by modulating protein aggregation and increasing cellular vulnerability. However, the precise …
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Defining the genetic and molecular pathways underlying motor neuron degenerative diseases
… and potential of a novel cholesterol metabolite (oxysterol) biomarker blood-based assay for MNDs, chapter five details the development of methods to obtain subcellular (mitochondria and ER) fractions from blood samples and cultured cells for oxysterol quantification. These methods were used to …
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Studies on Bioactive Lipid Mediators Involved in Brain Function and Neurodegenerative Disorders. The effect of ¿-3PUFA supplementation and lithium treatment on rat brain sphingomyelin species and endocannabinoids formation; changes in oxysterol profiles in blood of ALS patients and animal models of ALS.
… endocannabinoids (EC). These lipid mediators and oxysterols elicit potent bioactive functions in many physiological and pathological processes of the brain and neuronal tissues. They have been investigated for biomarker discovery of ageing, neuroinflammation and neurodegenerative disorders. The …
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Targeting Plasma Membrane Phosphatidylserine Content to Inhibit Oncogenic Kras Function
… is sufficient to abrogate KRAS oncogenesis. Oxysterol-related binding proteins ORP5 and ORP8 exchange PtdSer synthesized in the ER for phosphatidylinositol-4-phosphate (PI4P) synthesized in the PM. We show that depletion of ORP5 or ORP8 reduced PM PtdSer levels, resulting in extensive …
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Sequential Actions of VCP/p97 and the Proteasome 19S Regulatory Particle in Sterol-Accelerated, ER-Associated Degradation of HMG CoA Reductase
… geranylgeraniol. Here, we report that the oxysterol 25-hydroxycholesterol and geranylgeraniol combine to trigger extraction of reductase across ER membranes prior to its cytosolic release. This conclusion was drawn from studies utilizing a novel assay that measures membrane extraction of …
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Genetic Determinants of Human Serum Sterol Levels
… concordant results. Direct sequencing of the oxysterol 7α-hydroxylase gene (CYP39A1), which uses 24-hydroxycholesterol as a substrate, identified several mutations that decrease enzyme activity and thus lead to increased serum levels of this sterol substrate. In the genetic linkage analysis, a …
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Anti-Inflammatory Mechanism By Oxysterols In Cultured Rat Brain Astrocytes
… are enriched in the brain and can be oxidized to oxysterols by spontaneous or enzyme-mediated processes. Oxysterols are transport forms of cholesterols across cell membranes and the blood-brain barrier. Here, to elucidate the roles of oxysterols in brain inflammation, I treated …
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Recruitment and function of ORP1L on the Coxiella burnetii parasitophorous vacuole
… role of cholesterol-binding host protein ORP1L (Oxysterol binding protein-Related Protein 1, Long) during infection. ORP1L is a multi-functional cholesterol-binding protein involved in late endosome/lysosome (LEL) trafficking, formation of membrane contact sites between LEL and the endoplasmic …
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Role of Macrophage Apoptosis in Atherosclerosis.
… Bax also occurs in free cholesterol-loaded and oxysterol treated mouse macrophages. In this study, we evaluated the influence of Bax deficiency on apoptosis in macrophage-like P388D1 cells by using small interfering RNA (siRNA) to suppress Bax expression, as well as in peritoneal macrophages …
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Role of Cholesterol 24-Hydroxylase in Hippocampal Long-Term Potentiation
… the production of the membrane-permeable oxysterol 24(S)-hydroxycholesterol and represents the major pathway of cholesterol catabolism in this organ. Mice lacking 24-hydroxylase have a decreased rate of brain cholesterol synthesis and exhibit deficiencies in spatial, associative, and motor …
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Protein Sensors for Membrane Cholesterol
… Niemann Pick Type C2 disease protein and yeast oxysterol-binding protein homolog transport cholesterol between membranes. I also showed that two point mutations in Niemann Pick Type C2 disease protein that cause cholesterol storage disease also render the protein defective in transporting …
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Role of Type 2 Cannabinoid Receptor (CB2) in Atherosclerosis.
… found that CB2-null macrophages are resistant to oxysterol/oxLDL-induced apoptosis leading us to hypothesize that CB2 may modulate macrophage apoptosis in atherosclerosis. To determine the functions of CB2 in atherosclerosis, we fed low density lipoprotein receptor-null (Ldlr-/-) and Ldlr-/- mice …
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Gangliosides와 oxidized low density lipoprotein (oxLDL) 에 의한 신경교세포의 활성조절 및 그 기전에 대한 연구
… and STAT1 activation in Brain Microglia 49 4. Oxysterol/LXR Agonists Mimic Anti-inflammatory Effects of OxLDL on LPS-activated Microglia 54 IV. DISCUSSION 64 A. JAK-STAT Signaling Mediates Gangliosides-induced Inflammatory Responses in Brain Microglial Cells 64 B. Oxidized Low Density …
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Mammalian cell cultures as a model system for the determination of cytotoxicity induced by cholesterol oxidation products
Oxysterols are products of cholesterol oxidation, which may be produced endogenously or may be absorbed from the diet where they are commonly found in foods of animal origin. Oxysterols are known to be cyctotoxic to cells in culture and mode of toxicity has been identified as apoptosis in certain …
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