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Showing 1 to 20 of 43 for “"oxoglutarate"”.
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Biochemical and Structural Studies of AlkB: A 2-oxoglutarate Iron(II) Dependent Dioxygenase
… damage repair enzymes. AlkB is a direct repair 2-oxoglutarate iron-II dependent dioxygenase that oxidizes alkyl lesions occurring on the N1 position of purines and the N3 position of pyrimidines in both DNA and RNA. AlkB preferentially repairs 1-methyladenine and 3-methylcytosine lesions, but it …
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Molecular Studies to Investigate 4-hydroxy-2-oxoglutarate Metabolism Defects of Primary Hyperoxaluria Type 3
… PH3 results when mutations prevent 4-hydroxy-2-oxoglutarate aldolase (HOGA) from cleaving 4-hydroxy-2-oxoglutarate (HOG) into pyruvate and glyoxylate. It is proposed that the consequences of PH3 could be lessened by preventing conditions that favor oxalate production downstream of deficient HOGA …
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Defining the role of 4-hydroxy-2-oxoglutarate aldolase in hydroxyproline metabolism and primary hyperoxaluria
Aberrant glyoxylate metabolism is a hallmark of primary hyperoxaluria (PH). PH types 1 and 2 are the consequence of functional defects in alanine-glyoxylate aminotransferase (AGT) and glyoxylate reductase (GR), respectively. The resulting increase in oxalate excretion can lead to the formation of …
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Molecular and Biochemical Genetics of 2-Oxoglutarate-Dependent Dioxygenases Required for Flavonoid Biosynthesis in Arabidopsis thaliana
Three 2-oxoglutarate-dependent dioxygenases required for flavonoid biosynthesis were characterized in Arabidopsis thaliana. Genes encoding flavanone 3-hydroxylase (F3H), flavonol synthase (FLS), and leucoanthocyanidin dioxygenase (LDOX) were cloned and sequenced. The predicted proteins encoded by …
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Examination of 2-Oxoglutarate Dependant Dioxygenases Leading to the Production of Flavonols in <i>Arabidopsis thaliana</i>
… and pharmacological activities in animals. The 2-oxoglutarate-dependant dioxygenases(2-ODDs), flavonol synthase (FLS) and flavanone 3-hydroxylase (F3H), are essential for flavonol synthesis. The primary goal of this study has been to gain a deeper understanding of the biochemistry of these enzymes …
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GC-MS Investigation of Metabolic Activity in 4-Hydroxy-2-Oxoglutarate Aldolase Deficiency of Primary Hyperoxaluria Type 3
… by a malfunction in the enzyme 4-hydroxy-2-oxoglutarate aldolase (HOGA), which catalyzes the cleavage of 4-hydroxy-2-oxoglutarate (HOG) to form glyoxylate and pyruvate. Since the enzymatic malfunction occurs before glyoxylate formation, and previous studies have shown HOG to be elevated in …
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Escherichia coli 2-oxoglutarate dehydrogenase multienzyme complex: e1 and e2 substrate specificty, e1 carboligase activity, and e2 interchain succinyl transfer
Escherichia coli (E. coli) 2-oxoglutarate dehydrogenase multienzyme complex (OGDHc) contains three components: a thiamin di phosphate (ThD P) dependent 2-oxogl utarate dehydrogenase (E1 o), a di hydrol i poylsucci nyl transferase (E2o), and a di hydrol i poyl dehydrogenase (E3). The first two …
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Biochemical and Genetic Characterization of Reduced Phosphorus Metabolism in Pseudomonas Stutzeri WM88
… that htrA encodes a novel member of the 2-oxoglutarate dependent dioxygenase enzyme family, htxBCDE encode a binding protein dependent hypophosphite transporter and that htxF-htxN encode the components of a Carbon-Phosphorus lyase for the degradation of phosphonate compounds. Sequence …
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The role of ABHD11 in the regulation of the hypoxia inducible transcription factors
… members of a diverse group of enzymes termed 2-oxoglutarate-dependent dioxygenases. These enzymes all require oxygen, iron and the Tricarboxylic Acid (TCA) cycle metabolite, 2-oxoglutarate (2-OG/a-ketoglutarate) for catalytic activity. Therefore, understanding how 2-OG levels are regulated is …
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In situ Metabolism and Biogeography of Phototrophic Consortia
… were strongly attracted by sulfide and 2-oxoglutarate, which indicated a potential role of these compounds in the metabolism of "P. roseum". In chemocline water samples, 2-[14C(U)]-oxoglutarate was utilized at nanomolar concentrations (half saturation constant of uptake Kt ≤ 10 - 40 nM), …
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Shared Metabolic Pathways in Fuel-Stimulated Insulin Secretion
… in the pyruvate/isocitrate cycle is cytosolic 2-oxoglutarate (2OG). In an effort to better understand the components of the pyruvate/isocitrate cycle and the signals that it generates, we initially focused our studies on the transporter protein responsible for the return of 2OG to the …
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Structural insights into conformationally-gated reactions catalyzed by thiamine pyrophosphate-dependent enzymes
… with carbonyl carbon(s), such as pyruvate and 2-oxoglutarate, two central metabolites. This thesis presents structural analyses of three TPP-dependent enzymes. Most interestingly, each mechanism of three proteins studied contains a gated step that is drastically accelerated by 3-5 orders of …
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Characterising mechanisms of regulation of hypoxia-inducible factors.
… hypoxia, such as iron chelators and analogs of 2-oxoglutarate, prevented hydroxylation of the asparagine allowing the CAD to interact with the transcriptional coactivator protein p300. We then demonstrate that the protein factor inhibiting HIF-1 (FIH-1), previously shown to interact with HIF, is …
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Lipoic acid assembly on 2-oxoacid dehydrogenases in E.coli
… We report that the intact pyruvate and 2-oxoglutarate dehydrogenase complexes specifically copurify with both LipB and LipA. Proteomic, genetic and dehydrogenase activity data indicate that all of the 2-oxoacid dehydrogenase components are present. In contrast LplA, the lipoate protein …
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A Mutant of Arabidopsis Thaliana Deficient in Chloroplast Dicarboxylate Transport Activity
… oil filter centrifugation technique. Malate, 2-oxoglutarate, glutamate and aspartate uptake were severely reduced or missing in chloroplasts from the mutant.
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Mechanisms of Enzyme Neofunctionalization in Plant Specialized Metabolism
… two enzyme families: nonheme iron(II)- and 2-oxoglutarate-dependent halogenase and BAHD acyltransferase. First, we describe the genomic- and biochemical-basis for gene duplication and neofunctionalization of flavonol synthase (FLS) that led to the unique emergence of halogenase in …
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ENVIRONMENTAL BENEFIT AND BURDEN OF POSTBIOTICS IN ANIMAL NUTRITION
… tricarboxylic acid (TCA) cycle interme-diates (2-oxoglutarate and citrate) in untargeted metabolomic treated cecal samples may point to a slight shift in the bacterial community, which metagenomic approaches might be able to explain in the future. The goal of the second trial was to examine the …
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