Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 342 for “"over-expression"”.
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Functional Consequences of Matrix Metalloproteinase-1 Over-Expression in Human Gliomas
… with MMP-1 cDNA. Confirmation of MMP-1 over-expression in these cells was achieved through real-time PCR and Western blot analysis. The functional consequences of MMP-1 over-expression were analyzed using a collagen type-I invasion assay along with clonogenic and ATP viability assays. …
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Over-expression and characterization of four alginate lyases from vibrio splendidus 12B01
… to be between 0.13 and 0.83 μM s -1 . The turnover numbers for the lyases was found to be between 0.60 and 7.1 s -1 .
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Transcriptional Regulation of an Extrachromosomal Gal7 Gene in Saccharomyces Cerevisiae (Yeast Plasmids, Regulatory Mutants, Over Expression)
… on autonomously replicating plasmids. Normal expression of the plasmid born GAL7 gene could occur in the absence of DNA encoding the functional genes of the GAL cluster. The single copy centromeric and chromosomal locations of GAL7 were indistinguishable in their response to induction by …
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Over-expression in Escherichia coli of the ToxA51 llama single domain antibody targeting Clostridium difficile enterotoxin A
… we investigated various parameters affecting the expression of the ToxA5.1 sdAb in E. coli TG1 and optimization of culture conditions revealed that temperature and medium buffering are critical parameters affecting small-scale expression of this recombinant protein. Large-scale expression was …
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Over-expression of NRF-1 in C2C12 myotubes increases GLUT4 content via a transcriptional cascade involving MEF2A
Previous studies have shown that over-expression of nuclear respiratory factor (NRF)-1 in mice increases glucose transporter (GLUT)-4 and myocyte enhancer factor (MEF-) 2A content, but the mechanisms have not been elucidated. Because NRF-1 has a binding site on the mef2a gene, and MEF2A binds the …
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Asthma: Cytokine-Induced Over-Expression of Matrix Metalloproteases Compromises Airway Epithelium Tight Junctions and a Clinical Case Study
… a result of cytokine activity that up regulates expression of matrix metalloprotease 9. MMP9 encourages detachment-induced cell death - anoikis - which results in a structural change compromising the airway epithelium's ability to act as a primary immune defense mechanism. The chronic …
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Metabolic engineering of the pterin branch of folate synthesis by over-expression of a GTP cyclohydrolase I in peanut
… efficiencies and regeneration potential. Expression levels of the AtGCHI transgene were determined by quantitative real-time PCR. The endogenous peanut GCHI (AhGCHI) was isolated and sequenced. Studies were conducted to test whether heterologous over-expression of AtGCHI altered expression …
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Investigating the coordination of germline stem cell fate by ecdysone signaling and BMP signaling in the Drosophila ovary
… signals that promote GSC self-renewal. Over-expression of Ecdysone Receptor (EcR), knock-down of bag of marbles (bam), or over-expression of constitutively active thickveins (tkv) in all germ cells results in germ cell tumors at similar stages of differentiation. This suggests that …
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Regulation of C-type lectin-like receptors dectin-1 and CLEC-2 by tetraspanins
… assay in the Jurkat T-cell line, transient over-expression of CD37 was found to powerfully inhibit dectin-1 signalling following stimulation with its ligand, β-glucan. Over-expression of other tetraspanins also inhibited dectin-1 signalling, but did not globally inhibit receptor signalling …
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The role of RTNLB3 in endoplasmic reticulum structure and cytokinesis in planta
… at the cell plate during its formation and that over-expression of a non-functional truncated form of the protein interferes with cell wall formation. Over-expression of truncated RTNLB3 results in an increased proportion of cisternal ER within the ER network. Stable over-expression of the …
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Mechanism of metastasis of triple negative breast cancer
… Several protein candidates show differential expression between metastatic and non-metastatic tumours. This research had hypothesised that over expression by the group of proteins increases the metastatic propensity of TNBC. In this research, I studied selected protein candidates extracted …
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Altered regulation of adipogenesis with respect to disease processes
… of wild-type (WT) and mutant (R482W) LMNA on the expression profile of differentiating 3T3-L1 mouse preadipocytes and identified over 200 transcripts whose expression was altered. Four of these genes namely ITM2A, IGFBP5, PTPRQ and WNT6 were selected for detailed investigation using the 3T3-L1 …
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Perfil de utilização do trastuzumabe no tratamento do câncer de mama
… 2013 to treat non-metastatic breast cancer with over expression of HER2. The aim of this study was to evaluate the diffusion and patterns of use of trastuzumab in the treatment of non-metastatic breast cancer in the Brazilian Southeast Region. The production of chemotherapy for breast cancer …
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Re-replication in the Absence of Replication Licensing Mechanisms in Drosophila Melanogaster
… by either RNAi depletion of geminin or Dup over-expression in cultured Drosophila Kc167 cells. In agreement with previously reported re-replication studies, I found that not all sequences were sensitive to geminin depletion or Dup over-expression. Microarray analysis and quantitative PCR …
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Trim24 As An Oncogene In The Mammary Gland
… people to breast cancer have been uncovered, we are just beginning to understand the extent to which epigenetic regulators promote tumorigenic phenotypes, metastasis, and chemotherapeutic resistance. Moreover, new experimental tools offer the ability to address questions we were …
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ROLE OF G9A IN SKELETAL MYOGENESIS
… that the lysine methyltransferase G9a represses expression of several sarcomeric genes, whereby over-expression of G9a disrupts sarcomere integrity of skeletal muscle cells. This inhibition is mediated by G9a catalyzation of repressive histone methylation marks at sarcomeric gene promoters. It …
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DNA repair in bacteroides fragilis
… activity of oxidation/reduction enzymes, over-expression of multidrug efflux pumps and the conversion of metronidazole to non-toxic derivatives by nitroimidazole nitroreductases (encoded by nim genes). However, metronidazole resistance could also potentialIy be mediated by the …
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Characterization of HIV-1 Rev mutants
… observed in HIV infected cells or cells that over-express Rev. Watts et al. hypothesized Rev interacts with MTs by a mechanism shared with Kinesin-13 (Kin13) proteins owing to the presence of a shared amino acid sequence. Kin13 proteins are potent MT depolymerizing agents affecting MT behavior …
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