Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 25 for “"outer membrane vesicles"”.
-
Bacterial Outer Membrane Vesicles. Mediators of virulence and antibiotic resistance.
Outer membrane vesicles (OMV) have the size of nanoparticles and are released from the outer membrane of most Gram-negative bacteria. The OMV reflect the composition of the bacterial outer membrane, including lipids and virulence factors specific for the pathogen, and are involved in pathogenesis …
-
Analysis and optimization of immune responses elicited by Outer Membrane Vesicles (OMVs)
Outer membrane vesicles (OMVs) are spheroidal particles released from all gram-negative bacteria and exert different biological functions. OMVs carry many microbe-associated molecular patterns (MAMPs) such as peptidoglycan, lipopolysaccharide (LPS), and lipoproteins and can be decorated with …
-
Bacterial outer membrane vesicles (OMVs) as a platform for personalized cancer vaccines
… preclinical models and human patients. Bacterial Outer Membrane Vesicles (OMVs) are naturally produced by all Gram-negative bacteria. They contain several Microbe-Associated-Molecular Patterns (MAMPs), crucial for stimulating innate immunity and promoting adaptive immune responses. The ability to …
-
Exploring the Polyclonal Antibody Responses Induced by Klebsiella pneumoniae Derived Outer-Membrane Vesicles
… are used by K. pneumoniae including a thick outer capsule, lipopolysaccharide (LPS), siderophores, and key metabolism and adhesion genes. These virulence factors assist in resisting a wide range of immunological and environmental stressors (complement, phagocytosis, antimicrobials, etc.). …
-
Studies on the synergistic protective activity of cancer antigens associated to engineered bacterial Outer Membrane Vesicles
Introduction Bacterial Outer Membrane Vesicles (OMVs) are naturally produced by all Gram-negative bacteria and are emerging as an attractive vaccine platform. The exploitation of OMVs in cancer immunotherapy was tested by decorating them with cancer epitopes and by following their capacity to …
-
Brucella abortus Strain RB51 Outer Membrane Vesicles as a Vaccine Against Brucellosis in a Murine Model
… available vaccine to prevent human brucellosis. Outer membrane vesicles have been tested as potential vaccines to prevent diseases caused by bacterial species. OMV are constantly released from Gram-negative bacteria. They are comprised principally of the outer membrane components and periplasmic …
-
Bacteroides fragilis outer membrane vesicles are used for secretion of a discrete subset of bacterial RNAs that stimulate an immune response in colonic epithelial cells.
… RNAs) that are carried as cargo in bacterial outer membrane vesicles (OMVs). The mechanisms by which human extracellular RNAs elicit immune responses have been well established, while the contribution of bacterial sRNA to host physiology remain unclear. We hypothesize that pathogenic and …
-
Characterization of OmpT: An outer membrane vesicle protease Escherichia coli that attenuates blood coagulation.
… O86a:K61 secretes the protease OmpT as part of outer membrane vesicles (OMVs), which inactivates coagulation Factor V and in so doing, induces a hemorrhagic state. To further characterize the effect of OmpT on the coagulation, this study has employed a genetic approach using wild type, an OmpT …
-
HcpE, a potential immuno-modulatory protein from Helicobacter pylori that is dependent on the Disulfide bond protein DsbHP
… as a soluble protein and in association with outer membrane vesicles, and may play a role in the modulation of H. pylori inflammatory responses. We identified that DsbHP is necessary for HcpE production and secretion in H. pylori, and demonstrated DsbHP has DiSulfide Bond (Dsb) forming …
-
Electrochemical and genetic tools for analysing and engineering bioenergetic processes in cyanobacteria
… cofactors found within the specialised thylakoid membranes of photosynthetic organisms. Cyanobacteria, to our knowledge, are the first organisms to have evolved oxygenic photosynthesis, and contribute approximately 25% of the Earth’s primary photosynthetic productivity. Cyanobacteria are also …
-
Genetic technologies to engineer and understand the microbiome
… These tools were then expanded to engineer outer membrane vesicles derived from Bacteroides as immunomodulatory agents. Finally, we leveraged the natural sensing abilities of bacteria to create cellular biosensors for biomarkers of gastrointestinal disease. Heme biosensors were paired with …
-
The Effect of O Antigen Loss on the Protein Composition and Inflammatory Response Elicited by Klebsiella pneumoniae
… bacteria, K. pneumoniae naturally release outer membrane vesicles (OMVs) during all stages of cellular growth. OMVs are composed of the outer membrane components such as lipopolysaccharide (LPS) and outer membrane proteins and contain cytosolic and periplasmic proteins in the lumen. K. …
-
Mechanism of Host Recognition by the Bacteriophage <i>Cystoviridae</i>
… double stranded RNA bacteriophage that fuses its outer viral membrane with the lipopolysaccharide membrane of its Gram-negative host. In a series of studies, we determined the specifics of <em>Cystoviridae</em> host binding and the requirements of host fusion. The outer membrane vesicles (OMVs) …
-
The conserved dedA family of E. coli membrane proteins: genetic and topological analysis
… conditional growth defects and alterations in membrane composition. A temperature-sensitive mutant named Lud135 was isolated with mutations in two related, nonessential genes: yghB and yqjA. yghB harbors a single missense mutation (G203D) and yqjA contains a nonsense mutation (W92TGA) in …
-
Assessing Helicobacter pylori's HcpE and DsbK's Effects on Immunostimulation in Host Gastric Cells
… live bacteria, bacterial culture supernatants, outer membrane vesicles (OMVs), supernatants devoid of OMVs of wild-type and knock-out mutants of DsbK and HcpE in two HP background strains or purified recombinant HcpE (rHcpE), followed by IL-8 secretion assessment. The relative expression levels …
-
Hydrodynamics and Diffusion of Membrane Proteins to Engineer Chemical Reactions in Synthetic Biomembrane Platforms
… used to characterize biological phenomena in membranes and to engineer those phenomena for applications such as sensing and separations. While microfluidic SLB platforms have developed considerably recently, extending the capabilities of such platforms for use in mimicking and engineering …
-
Approaches towards therapeutic development against chronic brucellosis in a mouse model
… the action of drugs that poorly cross cell membrane barriers. Additionally, when the immune response fails to clear the infection, chronic brucellosis ensues that becomes more challenging to treat with antibiotics. Therefore, two approaches, intracellular drug delivery and immunostimulation, …
-
Construction and characterization of proteome-minimized OMVs from E. coli and their exploitation in infectious disease and cancer vaccines
Bacterial Outer Membrane Vesicles (OMVs) are naturally produced by all Gram-negative bacteria and play a key role in their biology and pathogenesis. Over the last few years, OMVs have become an increasingly attractive vaccine platform for three main reasons. First, they contain several …
-
Survival strategies of the human respiratory tract pathogen Haemophilus influenzae
… pathway of the complement system. Mapping of the membrane bound Hsf with gold-labelled specific antibodies in transmission electron microscopy (TEM) revealed a double-folded 100 nm long fibrillar structure. Using a series of mutants, we showed that when the C-terminal translocator domain was …
-
Identification and characterization of a novel capsule-like complex surface antigen of Francisella tularensis
… it was composed of an increase in production of outer membrane vesicles and tubules (OMV/T). These OMV/T appeared to be self-aggregating into what I visualized through TEM as the CLC. LVSΔ1423-22 was attenuated in the mouse model, and BALB/c mice immunized with CLC and adjuvant were protected …
Page 1 of 2