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Showing 1 to 20 of 65 for “"osteocalcin"”.
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FRACTURE HEALING IN A RABBIT MODEL: A STUDY OF OSTEOCALCIN LOCALISATION
Immunocytochemistry was used to localise osteocalcin during fracture healing in tissue sections of adult rabbit tibiae. A peroxidase anti-peroxidase technique and an indirect peroxidase technique were assessed on newly developing bone in human fracture callus. Three antibodies which cross-reacted …
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Non-autonomous regulation of bone mass accrual and the role of T-cell protein tyrosine phosphatase in the bone regulation of insulin sensitivity
… also an endocrine organ, releasing the hormone osteocalcin that increases insulin secretion and sensitivity in the pancreas and testosterone production in the Leydig cells of the testis. In my thesis study, I explore both the cell-nonautonomous regulation of osteoblast differentiation and the …
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조골세포 사멸 및 분화에서 GSK3b의 역할
… cells, an osteoblast cell line. Dex decreased osteocalcin mRNA level compared to that of un-treated cells at an early differentiation period. Interestingly, Dex continuously accumulated the expression of heat shock protein 25 (HSP25). Gene knock-down of HSP25 by siRNA recovered osteocalcin …
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Are Bone Turnover Markers and Vitamin D levels associated with Frequency of Complete Denture Relines?
… turnover markers; C-terminal Telopeptide (C-Tx), Osteocalcin (OCN) and Vitamin D (25 OH-Vit D) levels to determine the presence of and the degree of association between them.</p> <p>METHODS: This study was designed as a cross-sectional study wherein a retrospective chart review of three hundred …
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Knochenzellwachstum im dreidimensionalen Zellkultursystem
… Nachweise für Prokollagen I, Kollagen I und IV, Osteocalcin, Osteonectin, und Versican waren in den 3-D-Präparaten in allen Zeiträumen deutlich positiv, die Nachweise für Alkalische Phosphatase und Decorin negativ. Im Vergleich zu den 3-D-Kulturen war in der stimulierten 2-D-Kultur die Markierung …
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Charakterisierung von humanen mesenchymalen Stammzellen und Zellen der osteoblastären Differenzierungskaskade
… Proteinen ergaben Bone Sialoprotein, Osteocalcin, Decorin sowie Kollagen-IV und Kollagen-X unterschiedliche Färbemuster. Kollagen-IV und –X waren besonders in hMSC positiv, weswegen sie sich für eine Erkennung unreifer osteoblastärer Zellen anbieten. Bone Sialoprotein, Osteocalcin und …
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The synergistic effect of pulsed electromagnetic fields and demineralized bone matrix on the osteogenic differentiation of mesenchymal stem cells
… for expression of alkaline phosphatase (ALP) and osteocalcin. Results demonstrated that PEMF was able to induce an increased osteogenic response throughout the 21 day culture. ALP and osteocalcin were significantly increased for PEMF treated groups at the earlier day 7 timepoint. DBM contains …
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The osteogenic effects of zirconia and titanium surfaces on human osteoblasts
… alkaline phosphatase (ALP) activity, and osteocalcin expression over a 21-day period. Quantitative analysis revealed that zirconia surfaces supported equal or superior early-stage cellular responses compared to titanium, particularly in attachment efficiency and proliferation rates. While …
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Dietary calcium and cation-anion difference influences calcium status and bone remodeling in exercising and sedentary Arabian horses
… protein, albumin, parathyroid hormone (PTH), osteocalcin, and hydroxyproline. Radiographs of the left third metacarpal bone were taken. An aluminum step wedge exposed simultaneously was used as a reference standard for estimating bone mineral content (BMC) with an image analysis program. Bone …
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The role of boron alone or in combination with estrogen or PTH in the treatment of postmenopausal osteoporosis in ovariectomized rats
… trabecular plate density, and increases in serum osteocalcin, and trabecular plate separation. However, intermittent PTH injection increased the dried bone weight to body weight ratio, wall thickness of the femur, total bone ash, bone ash to dried bone or body weight ratio, bone Ca, P, and Mg, …
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Silver Doped Hydroxyapatite Coating on Titanium Surfaces and its Effect on Early Bone Response and Osseointegration
… and mineralization which is measured by osteocalcin activity were evaluated using human embryonic palatal mesenchyme cells (HEPM), an osteoblast precursor cell line. Initial bacteria adhesion was evaluated using an RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I …
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Signaling mechanisms that suppress the anabolic response of osteoblasts and osteocytes to fluid shear stress
… in the expression of the anabolic bone gene osteocalcin. Additionally, mechanical stimulation of osteoblasts and osteocytes by fluid shear stress further enhanced expression of osteocalcin when Src activity was inhibited. Importantly, fluid shear stress stimulated an increase in nuclear Src …
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Investigating Human Cell Influence on the Micromechanical Environment of a 3D-Printed Bone Tissue Model
… 4 and 7 days to assess mechanical changes, and osteocalcin ELISA was used to examine alterations in bone-specific protein expression. Voronoi scaffolds exhibited a decrease in stiffness over time, while TO scaffolds remained stable; Voronoi scaffolds maintained a significantly higher storage …
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Biomarkers of Bone Turnover in Vegetarians and Omnivores
… and of bone turnover—serum and urinary osteocalcin(OC), skeletal alkaline phosphatase, and propeptide-type-I-carboxyterminal-procollagen(PICP). In all subjects, biomarker levels were significantly lower in women ages 27-33yr compared to women 20-26yr and 34-38yr. Selected resorption and …
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Osteogenic and chondrogenic differentiation of rat bone marrow stromal cells in 3D culture with novel PCL-TCP scaffolds
… Kossa staining, heightened expression of ALP and osteocalcin; chondrogenic differentiation was confirmed by alcian blue staining and toluidine blue staining. In conclusion, it was demonstrated that PCL-TCP scaffolds with fibrin glue facilitate proliferation, anchorage, motility, and …
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Modulation of the osteoblastic differentiation of marrow stromal cells for bone tissue engineering
… markers, such as collagen type I and osteocalcin. When cultured on an ECM, osteoblastic differentiation was accelerated and enhanced through (1) earlier upregulation of osteopontin and osteocalcin; and (2) maintenance of a high level of expression of these and other osteoblast-specific …
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Effect of a Low-Carbohydrate, High-Protein Diet on Bone Mineral Density, Biomarkers of Bone Turnover, and Calcium Metabolism in Healthy Pre-Menopausal Females
… were conducted at weeks 0, 1-4, 6, and 12. Serum osteocalcin was analyzed at weeks 0, 1, 2, 6, and 12, while urinary NTx was analyzed at weeks 0, 1 and 2. Weight loss was significant at the end of 12 weeks in both diet groups (P < 0.05) but there was no Diet x Time interaction. Total proximal …
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Proliferation und Differenzierung der Zellkultursysteme hFOB 1.19 und C3H10T1/2 - BMP-2 unter dem Einfluss von nichtsteroidaler Antiphlogistika und einzeitiger Radiatio
… wie Alkalische Phosphatase, Prokollagen I und Osteocalcin die Möglichkeit, den humanen in vivo Osteogeneseprozess in vitro im Zellkultursystem zu imitieren. In der vorliegenden Studie gelingt es, im Rahmen der NSAR-Versuche die Schlüsselrolle des PGE2 sowohl im Knochenmetabolismus als auch bei …
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Exploration of Small Peptide Binding Interactions with Inorganic Surfaces Across Timescales
… experiments. Likewise, the α-1 fragment of osteocalcin was investigated via binding isotherms for its ability to bind to both hydroxyapatite and titania, and its method of binding to the later was studied through solid state NMR in order to determine a free and bound structure. From these …
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Organosilicate nanoparticles as gene delivery vehicles for bone cells
… in bone formation, such as alkaline phosphatase, osteocalcin, osteopontin and fibronectin was quantified. The results indicated that osteoblasts retained their phenotype after organosilicate nanoparticle ingestion. The levels of various cytokines expressed during inflammatory response remained low …
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