Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"osteoblastic differentiation"”.
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Modulation of the osteoblastic differentiation of marrow stromal cells for bone tissue engineering
… extracellular matrix (ECM) to influence the osteoblastic differentiation of marrow stromal cells (MSCs). The effects of an in vitro generated ECM on MSC osteoblastic gene expression under static culture indicated that MSCs differentiated down the osteoblastic lineage evidenced by significant …
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Effect of Mechanical Environment on the Differentiation of Bone Marrow Stromal Cells for Functional Bone Tissue Engineering
… matrix, can be achieved by the directed differentiation of progenitor cells such as bone marrow stromal cells (BMSCs). Part two of the engineering strategy, the biomaterial scaffold, can be fabricated with the appropriate mechanical properties using a synthetic polymer system with tunable …
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Multi-scale structural design of titanium implants for improved osseointegration
… constructs, and exhibit increased production of osteoblastic differentiation and maturation factors on porous constructs compared to solid substrates. Implants with trabecular porosity lead to vertical bone growth on rat calvaria, and osseointegrate in the rabbit femur. These results indicate …
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Bioresorbable Electrospun Tissue Scaffolds of Poly(ethylene glycol-b-lactide) Copolymers for Bone Tissue Engineering
… not influence cell behavior (i.e. proliferation, differentiation) and their surface topography is on a scale length larger than a cell, which is too large to affect cell adhesion or orientation. It is hypothesized that poly(α-hydroxy ester) tissue scaffolds can be made more bioactive by (1) …
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Application of Fluid Flow for Functional Tissue Engineering of Bone Marrow Stromal Cells
… 3D scaffolds stimulates phenotypic markers of osteoblastic differentiation, but those studies did not determine whether the effects were a consequence of shear stress or increased nutrient availability. Consequently, this work has involved studies in a planar geometry, where nutrient effects …
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Effect of Implant Surface Microtopography by Hydroxyapatite Grit-Blasting on Adhesion, Proliferation and Differentiation of Osteoblast-like Cell Line, MG-63
… the in vitro osteogenesis, we experimented the osteoblastic cell adhesion, proliferation and differentiation using the human osteoblast-like cell line, MG-63 cells. Osteoblastic cell proliferation was performed to time-course. Also osteoblastic cell differentiation was verified by four different …
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Factors affecting bone cell growth and differentiation under differing culture conditions
Marrow stromal cell (MSC) differentiation into osteoblasts is an important part of the bone growth and remodeling process. This process can be exploited to help solve the problem of bone wound healing. Because of problems with bone grafts, implantation of biodegradable 3D scaffolds seeded with MSCs …
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Osteoblast-like cell response to titanium coated with co-immobilized Elastin-like polymers containing integrin-binding and Statherin-derived biomimetic peptides
… and SNa15) on titanium surfaces could improve osteoblastic responses. RGD is a peptide sequence important in binding nearly half of all integrins and is important in cellular adhesion. SNa15 is a modified peptide sequence from the N-terminus of the salivary protein statherin, which has been …
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Osteogenic Scaffolds for Enhanced Graft-Bone Integration in Ligament Tissue Engineering
… – that have the capacity to differentiate into osteoblastic cells – were able to attach and proliferate on all films yet appeared to do so to a greater extent on surfaces with higher heparin contents. Additionally, markers of osteoblastic differentiation were significantly higher on surfaces …
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Investigation of adipose-derived mesenchymal stem cells interaction with electrospun demineralized bone matrix nanofiber scaffolds
… on DBM scaffolds. The ability to support osteoblastic differentiation of AD-MSCs for long-term (three weeks) in osteogenic differentiation media was also investigated. Both PCL scaffolds and DBM scaffolds seeded with no cells were used as control surfaces. The total protein content of …
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Examination of Glucocorticoid Treatment on Bone Marrow Stroma: Implications for Bone Disease and Applied Bone Regeneration
… attributed to a steroid-induced decrease in the osteoblastic differentiation of multipotent progenitor cells found in the bone marrow. In order to determine if there is a change in the osteogenic potential of the bone marrow stroma following glucocorticoid treatment, Sprague-Dawley rats were …
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THE ROLE OF ENDOTHELIAL CELLS IN VASCULAR CALCIFICATION
… Fibronectin. Starting from day 5 high-Pi induces osteoblastic differentiation with RUNX2 and BMP2 mRNA up-regulation up to day 7, osteopontin increased protein expression and positive Alcian Blu staining. In calcified ECs mesenchymal and endothelial markers, such as α-SMA, SM22α, FSP-1 and …
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Runx2 and Vascular Calcification
… SMCs can undergo lineage reprogramming toward osteoblastic and chondrocytic fates during vascular calcification, we propose to determine whether SMC-specific Runx2 expression is required for development of AMC and AIC. We developed a mouse with smooth muscle-specific removal of Runx2 …
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Design, Fabrication, and Characterization of Three Dimensional Complete Scaffolds for Bone Tissue Engineering
… for 24hr were found to better support osteoblastic differentiation and mineral deposition.
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Mechanism of Mitogenic Action of Aluminum Ion on Bone Cells : Potential Involvement of the Insulin-Like Growth Factor Regulatory System
… cellular alkaline phosphatase activity (an osteoblastic differentiation marker) in osteoblast-like cells of chicken and human origin. Although biphasic, dose-dependent stimulations were highly reproducible, the maximal stimulatory dose varied among different experiments. Mitogenic doses of …
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Regulation of the Insulin-like Growth Factor System by Retinoic Acid in Human Osteoblast Cells
… (RA) is an important regulator of growth and differentiation in many cell types, including bone. However, its effects on human osteoblast cell growth and differentiation have not been well studied. Therefore, I investigated the effect of RA on proliferation and differentiation of normal human …
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The Fruit of Poncirius Trifoliata Promotes Mesenchymal Stem Cell Differentiation into Osteoblasts in Glucocorticoid-induced Osteoporosis Model
… of osteoblast and animal model. Effects of osteoblastic differentiation were measured by alkaline phosphatase (ALP) activity, osteopontin (OPN) protein expression and mineral nodule formation in dexamethasone (DEX)-treated MC3T3-E1 cells and primary bone marrow mesenchymal stem cells …
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Isolation and characterisation of canine osteosarcoma cells to investigate mechanisms of chemoresistance
… alkaline phosphatase (ALP) assay and qPCR on differentiation markers were performed to determine the osteoblastic characteristics of the cells. To determine the chemosensitivity of the three cell types to doxorubicin and carboplatin, drug response assays were carried out. qPCR was carried out …
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The regulation of TGFβ/BMP signalling by deubiquitylating enzymes
… impacts BMP-induced SMAD1 phosphorylation, mouse osteoblastic differentiation and Xenopus embryogenesis.<br/>A proteomic approach identified O-GlcNAc transferase (OGT) as an interactor of SMAD2. SMADs have not been associated with O-GlcNAc modifications and the regulation of TGFß/BMP signalling by …
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The Use of Dynamic Fluid Flow Strategies for Bone Tissue Engineering Applications
… It has been shown to enhance the deposition of osteoblastic matrix proteins in vitro, and is particularly important for the delivery of oxygen and nutrients to cells within large scaffolds suitable for bone tissue regeneration. In particular, dynamic flow profiles have been shown to be more …
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