Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"osmolyte"”.
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The Elasmobranch-Microbe Relationship: Trimethylamine n-oxide Synthesis, Urea Hydrolysis, and Microbe-Osmolyte Interactions in the Atlantic Stingray, <i>Dasyatis sabina</i>
… (TMAO). Despite the abundance of these plasma osmolytes, it is notable that elasmobranchs appear to lack urease and TMA oxidase (Tmase), enzymes that scavenge urea-nitrogen and synthesize TMAO, respectively. However, urease and Tmase are found in many species of bacteria. Therefore, I …
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Upgrading the Protein Formulation Toolbox through Predictive and Explanatory Models
… learning. From an explanatory standpoint, osmolyte-induced protein stability changes are re-contextualised through graph theory. A protein graph descriptor is identified as a highly sensitive metric to observe room temperature osmolyte-induced protein stability changes, outperforming …
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Control of perineurial glial growth in Drosophila melanogaster
… ine, which encodes a putative neurotransmitter/osmolyte transporter; eag, which encodes a potassium channel; and NF1, which encodes neurofibromin and is the Drosophila ortholog of the human gene responsible for Neurofibromatosis type1. I provide evidence that neurofibromin, in accordance with …
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Short-term recovery from a volume increase in the mouse zygote: Characteristics of regulatory volume decrease in vitro.
… zygotes was a swelling-activated Cl -/organic osmolyte channel like that found in many other cells. A method developed by Passantes-Morales et al. (1993) was used, in which a large extracellular concentration of one of a number of organic osmolytes was added to the experimental media, and the …
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Noncovalent Interactions Involving Microsolvated Networks Of Trimethylamine N-Oxide
… of hydrogen-bonded networks with the important osmolyte trimethylamine N-oxide (TMAO). Vibrational spectroscopy, in this case Raman spectroscopy, is used to interpret the effects of noncovalent interactions by solvation with select hydrogen bond donors such as water, methanol, ethanol and …
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Conformational dynamics of polypeptides and the folding of miniproteins studied by fluorescence spectroscopy
… sich auf die Fragestellung, wie organische Osmolyte die End-zu-End Kontaktformation von Peptiden und die Faltung des Tryptophan Cage beeinflussen. Unsere Ergebnisse zeigen, dass intrinsisch unstrukturierte Peptide nicht von Osmolyten beeinflusst werden, dass jedoch glutamindominierte Peptide …
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Physiological variation of Garry oak (Quercus garryana) seedlings to drought stress
… relative water content, and production of the osmolyte proline in response to drought. This study shows that seedlings from different populations of Garry oak have distinct physiological responses to drought that may lead to changes in survivorship for seedlings used in reforestation efforts …
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The Influence of the Cellular Environment on Z-DNA Formation
In the cell, chemically diverse solutes known as osmolytes accumulate in response to environmental stresses. To add to the understanding of how the environment inside a cell affects nucleic acid folding and function, we investigated the influence of cosolutes on the transition from B-DNA to Z-DNA …
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EFFECTS OF OSMOLYTES ON THE CONFORMATIONAL STABILITY AND HYDRODYNAMIC RADII OF IMMUNITY PROTEIN 9 AND HUMAN SERUM ALBUMIN
Abstract Osmolytes affect protein stability through direct interactions with a protein, indirectly by perturbing the properties of the solvent water, and by a combination of these. In this thesis the conformational stability of the colicin E9 immunity protein (Im9) and Human serum albumin (HSA) …
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Evaluating the resilience of Antarctic echinoderms to Southern Ocean freshening: Short- and long-term responses
… for low salinity acclimation. In particular, the osmolyte profile in both species differs from temperate echinoderms and other marine invertebrates, with branched-chain amino acids (valine, leucine, and isoleucine) potentially acting as both compatible osmolytes, cryoprotectants and even an energy …
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Determining the Roles of the Aldehyde Dehydrogenase Enzymes 5 and 6 during Icewine and Table Wine Fermentation in a Commercial Wine Yeast Using CRISPR-Cas9.
… glycerol production to act as an internal osmolyte to draw water back into the yeast cell, resulting in an NAD+ imbalance. Icewine has significantly higher acetic acid versus table wine, where acetic acid production may be used to reduce NAD(P)+ to NAD(P)H via NAD(P)+-dependent aldehyde …
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The role of Stl1p in glycerol accumulation in osmotically stressed icewine yeast Saccharomyces cerevisiae K1 V1116
… and accumulate glycerol as an internal osmolyte. In a laboratory strain of S. cerevisiae, STLl encodes for Stllp, an H+ /glycerol symporter that is glucose inactivated, but induced upon hyperosmotic stress. STLl, was found to be a highly upregulated gene in Icewine fermenting cells and …
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Fabrication and osmosis-mediated dynamics of a plant-inspired, fluid-filled, soft actuator
… or mesostructure of closed-cell, fluid-filled, osmolyte-driven active materials, I fabricate and characterize synthetic plant tissue analogs (PTAs). I create these analogs by encapsulating micron-sized saltwater droplets within thin Polydimethylsiloxane (PDMS) walls at concentrations of over 80% …
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Effects of Osmolytes on Amyloid-beta Protein Surface Activity and Membrane Interactions
… is highly crowded, with macromolecules and osmolytes occupying up to 40% of the cellular volume. The resulting molecular crowding and preferential exclusion modulate the thermodynamics of protein reactions to favor those that reduce total system volume and minimize solvent exposed surface …
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Roles of cosolvents on protein stability
… studies have focused on the effect of this osmolyte on protein backbone. Our results are consistent with these studies as we show that TMAO induces the backbone to adopt compact conformations. However, it is shown that effects of TMAO on the backbone are not dominant. In particular, TMAO's …
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Kinetic Characterization of the Coupled Folding and Binding Mechanism of Bacterial RNase P Protein: an Intrinsically Unstructured Protein
… coupled. Trimethylamine-N oxide (TMAO), an osmolyte that stabilizes the unliganded folded form of the protein, enabled us to study the folding process of P protein in the absence of ligand. Transient stopped-flow kinetic time courses at various final TMAO concentrations showed multiphase …
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Molecular mechanisms of salt and osmotic stress tolerance in Frankia strains isolated from Casuarina trees
… regulation, cell envelope remodeling, osmolyte biosynthesis, and signal transduction. Among the 153 genes shared only between the salt-tolerant strains, seven, including a zinc peptidase, were responsive to salt stress. Constitutive expression of the zinc peptidase gene in the …
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Gating of water channels (aquaporins) in plants: effects of osmotic and oxidative stresses and the role of unstirred layers
… within the water channel pores, when the osmolyte is present on both sides of the membrane. This should have affected the open/closed state by changing the free energy between states favouring a reversible distorted/collapsed state rather than the open. As expected from the C/T model, …
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The nanomechanics of polycystin-1: A kidney mechanosensor
… Urea is known to destabilize proteins. Other osmolytes found in the kidney such as sorbitol, betaine and TMAO are known to counteract urea¡¯s negative effects on proteins. Nothing is known about how the mechanical properties of PC1 are affected by these osmolytes. Here I use nano-mechanical …
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