Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"osmolarity"”.
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Physiological and genetic evidence for an OmpB signal transduction system in Erwinia chrysanthemi
… Some bacteria can survive fluctuations in medium osmolarity through the use of a two component signal transduction system. In Escherichia coli and Salmonella typhimurium this two component system includes the EnvZ sensor protein and its cognate response regulator, OmpR. The two genes that code for …
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Effects of size, age and photoperiod on hypoosmoregulation in brook trout, Salvelinus fontinalis
… were examined for possible influences on plasma osmolarity, [Na+], [Cl-], [K+], [Mg2+], thyroxine concentration, hematocrit, and gill Na+,K+-ATPase activity of brook trout in freshwater. Significant diel cycles were found in plasma osmolarity, (Na+] and thyroxine concentration. Significant size …
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The Regulation of Salmonella Typhi Vi Capsular Antigen Expression in Intestinal Model Epithelia and the Bovine Ligated-Ileal Loop Model
… resulted in an increase inflammatory response. Osmolarity is one of the control signals that affect the biosynthesis of the Vi antigen. Under high osmolarity growth conditions of 300 mM and greater, Vi production is suppressed and S. Typhi is highly invasive. Studies reveal that the viaB mutant …
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Role signální dráhy HOG MAPK při osmotickém stresu u Saccharomyces cerevisiae
… kinase (MAPK) signaling cascade (the high- osmolarity glycerol or HOG pathway) during conditions of increased external osmolarity. It evokes cellular responses necessary to permit continued growth. Activation of HOG pathway with Hog1 MAP kinase results in production of glycerol to prevent …
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Mechanics, shape, and programmability in soft matter systems: From fluid membranes to spring and droplet networks
… networks can be programmed with different osmolarity gradients. These osmolarity gradients generate internal stresses via local flows and the network then folds into designed structures. In other words, global change is driven by local osmolarity gradients. Using molecular dynamics …
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Osmoregulation at different stages of the yeast life cycle
… bakers’ yeast Saccharomyces cerevisiae, the High Osmolarity Glycerol (HOG) pathway is activated upon conditions of high osmolarity, and the pathway coordinates the responses needed to counteract loss of volume and turgor pressure. These actions include glycerol accumulation, ion efflux and …
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Evaluation of Different Concentrations of Egg Yolk in Canine Frozen Semen Extender
… (after freezing.) Initial values of pH, osmolarity, motility and morphology were obtained for comparison of the samples. Spermatozoa from six normal dogs as determined by progressive linear motility > 70% and normal morphology > 60% was used. Semen was collected and pooled for five …
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Cloning, Heterologous Expression, and Characterization of Human and Escherichia Coli Choline Dehydrogenases
… are exposed to drastic environmental changes in osmolarity. Betaine-glycine also plays an important role in osmoprotection in mammalian cells exposed to high extracellular osmolarity, such as kidney cells. Since CHDH is the first committed step of choline oxidation, it may be a critical …
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Elucidation of the osmoregulatory locus, ompRZ, in Erwinia chrysanthemi
… manner to OmpF and OmpC with respect to medium osmolarity. Furthermore, preliminary studies have shown that changes in osmolarity affect the expression of pathogenecity genes. Evidence for an osmoregulatory system analagous to the ompB system of Escherichia coli was, therefore, sought. Primers …
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Architecture and evolutionary stability of yeast signaling pathways
… that selection for the function of the High Osmolarity Glycerol (HOG) pathway has on the evolutionary stability of the pheromone response pathway in the yeast Saccharomyces cerevisiae. I first set out to demonstrate that, because the pheromone and HOG response pathways share protein …
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X-ray diffraction studies of muscle : observations on the anterior byssus retractor muscle of Mytilus edulis and the insect flight muscle from Sarcophaga bullata
… the interfilament spacing was dependent on the osmolarity of the bathing medium. In concentrated solutions of the artificial seawater, the interfilament spacing decreased; while in dilute solutions of artificial seawater, it was observed that the interfilament spacing was increasing. X-ray …
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Improving product release from saccharomyces cerevisiae and kluyveromyces lactis by pretreatment for cell wall weakening and selective product release
… yeast suspension into a pre-warmed pH or high osmolarity buffer. On dilution, the temperature was increased rapidly to 40CC, while the pH or osmotic pressure was increased to pH 10 or 1 MPa.
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Cellular mechanism of contractile dysfunction in diabetes-induced cardiomyopathy
… of increased extracellular (hyperosmotic) osmolarity on the contractility of ventricular myocytes in STZ-treated rat were also investigated compared to control. Diabetes was induced in male Wistar rats by a single i.p. injection of STZ (60mg kg', body weight) which, resulted in an …
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Exploring the Dynamics of Mechanosensitive Channels Utilizing a Post Translational Modification Approach
… touch, gravity, blood pressure, and changes in osmolarity. The bacterial mechanosensitive channel of large conductance (MscL) and the mechanosensitive channel of small conductance (MscS), from E. coli, have been the most studied mechanosensors to date. These channels coordinate a response to …
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Polyploidy in Saccharomyces cerevisiae leads to the loss of cell cycle control in stationary phase
… of stresses including oxidative stress, high osmolarity, salt stress, toxic ions, and growth at high temperatures. However, polyploid strains of S. cerevisiae rapidly lose viability under conditions of nutrient deprivation. In contrast to isogenic haploids that remain viable for weeks and even …
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The prevention of heparanase expression in endothelial cells injured by high glucose
… mannitol to determine if free radical injury or osmolarity caused effects similar to high glucose treatment. As well, high glucose or heparinase I treated PAECs were also treated with heparin (0.5 ìg/ml) and/or insulin (1 U/ml) and/or basic fibroblast growth factor (bFGF, 1 ng/ml) to determine if …
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Characterization of the Omega Subunit of Borrelia Burgdorferi’s RNA Polymerase
… changes such as shifts in pH, organic acids, and osmolarity levels. B. burgdorferi successfully adapts to these environmental cues through changes in gene expression carried out by RNA polymerase (RNAP). The RNAP holoenzyme consists of two ɑ subunits, a β subunit, and a β’ subunit. Additional …
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Molecular characterisation of the mechanisms of compatible solute accumulation in Listeria monocytogenes
… to survive and grow in environments of elevated osmolarity can be attributed, at least in part, to the accumulation of a restricted range of low molecular mass solutes compatible with cellular function. Accumulated to high internal concentrations in hyper-saline environments, compatible solutes, …
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Process development and scale-up from shake flask to fermenter of suspended and immobilized aerobic microorganisms
… starvation, anaerobic conditions, organic acids, osmolarity and pH. It is evaluated and compared with the standard measuring methods, exhaust gas analysis and respirometry. A simple and inexpensive shake flask screening method to perform immobilization studies with solid supports is introduced. …
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