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Showing 1 to 20 of 28 for “"ornithine decarboxylase"”.
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Regulation of the speC gene encoding ornithine decarboxylase in Escherichia coli by putrescine, spermidine and cAMP
… coli, the speC gene encodes biosynthetic ornithine decarboxylase (ODC), an enzyme that catalyzes the decarboxylation of ornithine to produce putrescine. The two polyamines, putrescine and spermidine, and the cyclic AMP (CAMP) - cAMP receptor protein (CRP) are known to inhibit the …
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THE REGULATION OF POLYAMINES AND THEIR ROLE IN THE PROLIFERATION OF HELA CELLS
<p>Ornithine decarboxylase activity was stimulated in high-density HeLa cell cultures by dilution of or replacement of spent culture medium with fresh medium containing 10% (v/v) horse serum. After stimulation, ornithine decarboxylase activity reached a peak at 4-6 h. then rapidly declined to the …
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Apparent induction, kinetic and physical properties of the multiple species of ornithine decarboxylase, forms A and B, in the kidney and liver of hormone-treated rat
Ornithine decarboxylase occurs in the kidney and liver of control and hormone treated rats as two ionic forms, designated ODC A and B in order of elution by DEAE-Sepharose chromatography. These studies were designed to better characterize the multiple forms of ODC and to investigate the functions …
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Characterization of the expression profile of polyamine biosynthetic genes (Spermidine synthase) and polyamine metabolic regulation in arabidopsis
… genes encoding other biosynthetic enzymes (mouse ornithine decarboxylase or Plasmodium falciparum ornithine decarboxylase/S-adenosylmethionine decarboxylase) altered spermidine/spermine content much, which indicates a complex and tightly regulated machinery for higher polyamine homeostasis. The …
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Characterization of the interaction of putrescine and the adenosine-3' ,5'-cyclic monophosphate-cAMP receptor protein complex in the regulation of the speC gene encoding ornithine decarboxylase in Escherichia coli
Ornithine decarboxylase (ODC) catalyzes the decarboxylation of ornithine to produce the diamine, putrescine, in the bacterium Escherichia coli. The speC gene encoding ODC has been shown to be subject to transcriptional repression by either putrescine or the cAMP- cAMP receptor protein (CRP) …
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Generation of nitric oxide and its protective and toxic actions in the gastrointestinal tract
… investigate the role of NO in the induction of ornithine decarboxylase in the rat gastric mucosa damaged by hypertonic saline in vivo, (iii) to expose primary cultures of guinea-pig gastric mucosal cells to oxidative challenge and an NO donor, and to investigate the response in terms of heat …
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The Role of Queuine Deficient tRNA in Translational Control
… genes were used; Nmyc, an oncogene, and murine ornithine decarboxylase, a growth-related gene. These proteins are produced in quantity in cells with hypomodified tRNA and have been shown to be subject to translational controls. Rabbit globin mRNA was used as a control.</p> <p>Nmyc cDNA was not …
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Estrogenic and Antiestrogenic Regulation of Cell Proliferation and Tissue Growth
… in the rat uterus. I studied the regulation of ornithine decarboxylase (ODC) activity and polyamine concentrations by E2 and antiestrogens (AE) as an aid to understanding the dissimilar effects of E2 and AE on uterine growth. Both E2 and AE stimulated ODC activity. AE were antagonistic to E2 in …
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ΜΕΛΕΤΗ ΤΗΣ ΒΙΟΣΥΝΘΕΣΗΣ ΤΩΝ ΠΟΛΥΑΜΙΝΩΝ ΣΤΑ ΒΛΑΣΤΗΜΕΝΑ ΣΠΕΡΜΑΤΑ ΚΡΙΘΑΡΙΟΥ
THE ACTIVITIES OF L-ARGININE DECARBOXYLASE (ADC), L-ORNITHINE DECARBOXYLASE (ODC AND ANTIZYME (AZ) IN GERMINATED BARLEY SEEDS ARE CONSIDERABLY AFFECTED BY THEPRESENCE OF PLANT HORMONES (GIBBERELIC ACID, CYTOKININS) IN THE GROWTH MEDIUM,ODC FROM CHROMATIN OF GERMINATED BARLEY SEEDS HAS BEEN PURIFIED …
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Polyamine metabolism in Arabidopsis: Transgenic manipulation and gene expression
… manipulation of polyamines via a mouse ornithine decarboxylase (mODC) gene on plant metabolism. (2) To analyze the spatial and temporal expression patterns of Arabidopsis thaliana S-adenosylmethionine decarboxylase genes (AtSAMDC3, AtSAMDC4 and AtSAMDC5) during its life cycle.</p><p>The …
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Understanding polyamine metabolism through transgenic manipulation in poplar suspension cultures
… nigra x maximowiczii), transformed with a mouse ornithine decarboxylase gene (under the control of a 2X 35S CaMV promoter) were used to study the impact of up-regulation of putrescine biosynthesis (and consequent enhanced catabolism) on several aspects of cellular metabolism. The transgenic cells …
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Trasduzione del segnale e poliamine nell'apoptosi di cellule cardiache
… the cells in the presence of α-difluoromethylornithine (DFMO). Polyamines were separated and quantified in acidic cellular extracts by HPLC after derivatization with dansyl chloride. Caspase activity was measured by the cleavage of the fluorogenic peptide substrate. Ornithine decarboxylase …
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Transgenic manipulation of polyamine metabolism in poplar
… cells (line 2E) showed elevated levels of mouse ornithine decarboxylase enzyme activity, several fold higher amounts of putrescine, a small increase in spermidine, and a small reduction in spermine as compared with non-transgenic (NT) cells. The conversion of labeled ornithine into putrescine was …
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The Role of Mga in the Survival of Pluripotent Cells During Peri-implantation Development
… a primitive endoderm marker. Expression of Ornithine Decarboxylase (ODC), the rate-limiting enzyme in the synthesis of cellular polyamines, was identified as a possible cause of embryonic lethality based on a similar mutant phenotype as well as the presence of E-box sequences in genetic …
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Establishment of CRISPR-Cas-based gene deletions in Bacillus methanolicus and metabolic engineering towards the production of N-methylglutamate and putrescine
… via two primary biochemical pathways: the ornithine decarboxylase (ODC) pathway and the arginine decarboxylase (ADC) pathway. N-Methylation of amino acids is a crucial post-translational modification that adds a methyl group to the nitrogen atom of an amino acid, significantly altering its …
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Recoding and reassignment in protists
… over 200 diverse protist species for the protein ornithine decarboxylase antizyme, a key negative regulator of cellular polyamine synthesis. The synthesis of this protein usually requires a +1 ribosomal frameshift at the end of the first open reading frame. In this study I identified a novel …
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The Role of S-Adenosylmethionine Decarboxylase on Regulation of Polyamine and Trypanothione Metabolism in Trypanosoma Brucei
… characterized T. brucei S-adenosylmethionine decarboxylase (AdoMetDC), a key enzyme required for the synthesis of spermidine and trypanothione, and examined the role of AdoMetDC on the regulation of polyamine and trypanothione metabolism. The recombinant T. brucei AdoMetDC enzyme displays low …
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PREVENTION OF CARCINOGENESIS AND CANCER CELL PROLIFERATION BY AN INHIBITOR OF POLYAMINE BIOSYNTHESIS (DFMO, HELA, MAMMARY, TUMOR)
… of a 1% solution of D,L-(alpha)-difluoromethyl-ornithine (DFMO), an inhibitor of polyamine biosynthesis, in drinking water for 6 months. This treatment significantly reduced cancer incidence and the average number of cancers per rat and prolonged cancer-free time. The average weight of mammary …
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CHANGES IN THE BIOCHEMICAL COMPOSITION OF TESTES DURING SPERMATOGENESIS IN ASTERIAS VULGARIS, WITH EMPHASIS ON THE ROLE OF POLYAMINES IN REGULATING PROLIFERATION
… of the germinal epithelium.</p><p>Activity of ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine synthesis, increases during the proliferative phase of spermatogenesis. Testicular ODC activity correlates well with DNA synthetic rate. To test the possible role of polyamines in …
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