Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"organoid model"”.
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Development of a Novel 3-Dimensional Testicular Organoid Model for the Study of Human Spermatogenesis and Gonadotoxicity In Vitro
… predictive capacity, and existing human in vitro models of testicular toxicity do not recapitulate the complex signaling interactions of the native tissue environment. The overall goal of this study was to develop, characterize, and evaluate a 3-dimensional (3D) human testis organoid culture …
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Molecular and Genetic Mechanisms in Atopic Eczema
… Immunol 2016). I optimised a well-established organoid model, using primary human keratinocytes and fibroblasts to recapitulate aspects of human skin for functional testing of specific genetic effects. Quantitative global proteomic analysis of the organoid model with FLG knockdown has …
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Host-Pathogen Interactions at the Intestinal Epithelial Barrier
… host-pathogen interactions a novel in vitro model of small intestine organoids was developed. Data suggest that immunoprotection against Eimeria vermiformis infections is mediated by T cells. Furthermore, there is an indication that ILCs have a detrimental effect in Eimeria …
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Characterization of A Novel Oncogenic Mirna Inhibitor In Cancer Therapy
… This effect is also shown in a cerebral organoid model. Using a multidisciplinary approach, this study identifies a relationship between oncogenic miR-10b and the dysregulation of the PI3K/AKT pathway in cancer, along with putting forth a compound that can mitigate these effects. These …
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Towards using organoids in functional genomics – A single cell characterisation of mouse gastruloids
… to perform such studies at scale in vivo, organoid models of mouse gastrulation are now making them possible. This thesis concerns the characterisation of an organoid model of mouse gastrulation called gastruloids, as a model for such perturbation studies. Gastruloids are grown by …
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Derivation of next generation research models of Parkinson’s disease
… understood. The development of advanced research models is pivotal in unravelling the mysteries surrounding PD's pathogenesis and progression. Although traditional monolayer cell culture models have significantly enhanced our understanding of the disease, their results have not consistently …
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Identification and characterisation of novel interactors of surfactant protein C
… of top hits in a novel CRISPR interference AT2 organoid model. I also undertook an exploration of the biology of MPZL1, a poorly characterised Ig-superfamily member which differentially interacts with, and is relocalised by, SFTPC-WT and I73T. This suggests that the normal function of MPZL1 may …
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Leveraging cellular models of polycystic kidney disease for mechanistic discovery and therapeutic development
… by limitations in currently available research models. Here we develop gene-edited clonal tubular epithelial cells and multi-cellular kidney organoids for mechanistic and therapeutic studies of PKD. In our kidney organoid model, we utilize CRISPR base editing to generate human pluripotent stem …
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The role of mucus in the cross talk between gut bacteria and the host
… a γδ T cell-deficient (TCRδ-/-) mouse model was used. TCRδ-/- mice showed increased susceptibility to dextran sodium sulphate (DSS)-induced colitis, alterations in mucin expression, glycosylation and goblet cell numbers, but maintained an intact mucus layer in vivo. Moreover, TCRδ-/- …
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A study of the causes and consequences of SFTPC mistrafficking in alveolar type 2 cells using novel organoid models
… by developing a genetically manipulable alveolar organoid model in which to test the consequence of ITCH depletion. Using this novel organoid system, I then generated models of both inducible exogenous SFTPC-I73T expression and its endogenous expression using base editing. These heterozygous …
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BRAIN ORGANOIDS AS MODELLING CONDUITS OF HUMAN NEURODIVERSITY: ADVANCED COMPUTATIONAL APPROACHES TO SINGLE CELL TRANSCRIPTOMICS
… processes. The introduction of human brain organoids (BOs), allowed to reach an unprecedented experimental tractability of brain development trajectories, surpassing the limitations of their preceding 2D models and of animal models in recapitulating CNS cytoarchitecture. The advent of single …
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Prevalence and fate of pro-oncogenic clones in the human colon
… CD4+ immune cells. Finally, a human colonic KO organoid model was used to study the clonal behaviours in vitro and gain insight into the potential mechanisms and pathways that could be mediating mutation-associated advantages. The normal human colon appears to be more mosaic in terms of …
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The Role of Cervical Reserve Cells in Epithelial Regeneration and Homeostasis in the Murine and Human Cervix
… after an injury (2). Developing an in vitro model that is truly representative of normal cervical epithelium is crucial to address basic questions about homeostasis mechanism and the role of reserve cells in HR-HPV infections. In recent years, advances in modelling the human tissue in 3D …
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3D KIDNEY ORGANOIDS AS A PLATFORM FOR INVESTIGATING MONOGENIC DISEASES AND GENE THERAPY APPROACHES
Abstract The use of 3D in vitro models is now widely adopted in biomedical research. These models enable the study of human cells under more physiologically relevant conditions and allow for early proof-of-concept testing of therapies prior to animal studies. While 3D models do not replace …
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The Role of Mesenchymal Cells in the Regeneration of Salivary Gland Tissue
… particularly when developing cell cultures and organoid models. 23 In order to investigate the impact of PSS on the ability of cells to proliferate and perform a rudimentary regenerative function, the group developed cell cultures that were representative of epithelial and mesenchymal cell types …
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Modelling human liver bud development in vitro
… is particularly apparent. Indeed, current models of hepatobiliary development remain extremely rudimentary, lack the cellular complexity of natural tissue and do not produce organ progenitors with full developmental potential. To address these major questions, I have developed a system …
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Investigating Parkinson’s Disease human iPSC-derived models through single-cell gene expression
… active development for clinical and disease modeling purposes. Directed differentiation protocols of iPSC to midbrain dopaminergic neurons enables the study human midbrain Dopamine(DA) neurons, the primary neuronal group affected in Parkinson's Disease(PD), in-vitro. Despite their increasing …