Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 140 for “"organoid"”.
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MICROGLIA-SUFFICIENT BRAIN ORGANOID
… lack of tissue access. The generation of brain organoids from induced human pluripotent stem cells (iPSCs) has been shown to recapitulate the human embryonic brain development process. However, such organoids lack microglia. Here, we generated microglia-sufficient brain organoids by co-culturing …
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Uncovering Constraints On Organoid Morphologies
Organoids recapitulate complex 3D organ structures and represent a unique opportunity to probe the principles of self-organization. While we can alter an organoid’s morphology by manip- ulating the culture conditions, the morphology of an organoid often resembles that of its original organ, …
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Characterisation of human enteroendocrine cells in organoid models
… knock-in to generate human intestinal organoids with fluorescently labelled EECs. Organoids are three dimensional self-renewing cultures grown from the adult stem cells of intestinal crypts which express mature epithelial cell types, including EECs. The properties of labelled human …
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Creation of organoid systems to model human endodermal organ development in vitro
Organoid systems have revolutionised the study of adult stem cells and organ homeostasis. However, there have been limited attempts to use the technology to study human organ development. The work of this thesis established two novel hepatic organoid systems; one based upon the archetypal liver …
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Investigating the movement of intestinal intraepithelial lymphocytes using an organoid co-culture system
… how these immune cells move.<br/><br/>Intestinal organoids are three-dimensional structures of epithelial cells that mimic the gut epithelium in vitro. In this study a murine IEL-organoid co-culture system was established to study IEL movement. It has been shown that it is possible to keep them …
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An organoid platform to study alveolar stem cells in lung generation and cancer
… into a malignant state. The development of organoid technology has revolutionized the way we model cancer and a vast number of other biological phenomena.
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Cardiac organoid technology and computational processing of cardiac physiology for advanced drug screening applications
… to explore the potentials of stem cell cardiac organoid models to recapitulate heart development and implement analytical computational tools to study cardiac physiology. These new tools were implemented as potential advancements in drug screening applications for better predictions of …
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Investigating circuit and spatial heterogeneity of human brain tumors with patient-derived organoid models
… heterogeneity by leveraging patient-derived GBM organoids (GBOs), a 3-dimensional model inspired by human brain organoids. In the first sections of this dissertation, I describe the application of circuit neuroscience techniques to study diverse tumor-neuron circuitry. Previous studies discovered …
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FROM A PLACODE PROGENITOR PLATFORM TO A TRIGEMINAL GANGLION ORGANOID: MODELLING SENSORY DEVELOPMENT AND FUNCTION IN VITRO
… can be differentiated towards three-dimensional organoids mirroring tissue development, morphology and function, but organoids of the TG are yet to be developed. Here, we aim at generating the first TG organoid (TGO) and at characterising its development, maturation and function. By mimicking TG …
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Innovations in biomedical optical imaging for translational medicine: from in-vitro organoid analysis to vascular mapping in dermatology
L'abstract è presente nell'allegato / the abstract is in the attachment
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Development of a Novel 3-Dimensional Testicular Organoid Model for the Study of Human Spermatogenesis and Gonadotoxicity In Vitro
… and evaluate a 3-dimensional (3D) human testis organoid culture system for use as both a novel predictive first tier drug-screening tool and as a model of human testicular function in vitro.
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A study of the causes and consequences of SFTPC mistrafficking in alveolar type 2 cells using novel organoid models
… by developing a genetically manipulable alveolar organoid model in which to test the consequence of ITCH depletion. Using this novel organoid system, I then generated models of both inducible exogenous SFTPC-I73T expression and its endogenous expression using base editing. These heterozygous …
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Exploring the role of BCL11A in the oesophagus and mammary gland using in vivo lineage tracing and 3D organoid models.
… vivo lineage tracing techniques and in vitro 3D organoids to explore the expression and gene function of Bcl11a in the oesophageal epithelium and mammary gland. Gene function studies were undertaken using mouse models of BCL11A overexpression: RosaCreERT2; Rosa-CAGG-LSL-BCL11A (BCL11AOE) and …
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Development of an in vitro human lung organoid system to study alveolar type 2 stem cell maintenance and dysfunction during disease
… developed and characterised a novel in vitro 3D organoid platform that allowed for long-term hAT2 maintenance in chemically-defined conditions. Cultured hAT2-derived organoids underwent clonal expansion, were functionally mature and exhibited maintenance of differentiation capacity. High Wnt …
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Cerebral Organoids as 3-Dimensional Grafts in Traumatic Brain Injury
… recent emergence has led researchers to examine organoids’ potential as tissue grafts to regenerate the lost tissues in the brain following traumatic brain injury. In this dissertation, we sought to understand the interaction between the organoid and host following transplantation as a tissue …
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Interaction between form and fate during human neural development
… during neural development. We relied on cerebral organoids as a tractable *in vitro* system where tissue shape can be manipulated following several complementary approaches and tissue identity assessed by transcriptomics. We first discovered key protocol variables that contribute to differences in …
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