Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 108 for “"optogenetics"”.
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Investigations into cellular signaling through optogenetics and nanotechnology
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-05-01
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Closed-loop bladder function control for rats using optogenetics
… sensors. A neuromodulation technique called optogenetics is used as the method of intervention to help control the bladder function. The battery-less implantable device is wirelessly powered via inductive resonance coupling to reduce the size of the implantable device by eliminating batteries …
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Advanced technologies for spatio-temporal control of neural circuits using optogenetics
The progress of neuroscientific research is linked to the development of technologies able to interface with the complexity of neural circuitry. Remarkable advances in this direction have been achieved in the past decades and it is now possible to optically control the neural activity of …
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Chemi-Optogenetics: Photoactivatable Probes To Control Protein Proximity And Cellular Processes
Chemi-optogenetics is a hybrid chemical and genetic approach that utilizes light to regulate cellular processes. We combined the chemical versatility of photoremovable protecting groups with the biological specificity of self-labelling proteins (i.e. Halo-tag, SNAP-tag, eDHFR), to develop a series …
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Development and application of control tools for use in optogenetics research
… is realized. This thesis provides a review of optogenetics and details the development and application of a novel wireless device to optically control neural circuits and behavior.
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Neuromast Optogenetics Reveals Rules of Spatial Encoding in the Zebrafish Lateral Line
… in this thesis that combines single-neuromast optogenetics with whole-brain calcium imaging in zebrafish larvae. By optogenetically stimulating individual neuromasts, I observe that second-order circuits in the medial octavolateralis nucleus (MON) exhibit diverse selectivity properties to …
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3-D optical waveguide arrays for in-vivo optogenetics : development and application
A key feature of neural circuits in the mammalian brain is their 3-dimensional geometric complexity. The ability to optically drive or silence sets of neurons distributed throughout complexly shaped brain circuits, in a temporally precise fashion, would enable analysis of how sets of neurons in …
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Functional Labeling of Individualized Post-Synaptic Neurons using Optogenetics and trans-Tango
… of Individualized Post-Synaptic Neurons using Optogenetics and trans-Tango, which is a method developed to fulfill all three of these conditions. FLIPSOT uses a pre-synaptic driver line to drive trans-Tango, triggering heat-shock-dependent expression of post-synaptic optogenetic receptors. When …
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A stimulus/recording system for optogenetics: circuit, firmware, and software design and implementation
… system is the crucial component in studying optogenetics in a living animal model. In this study, a lightweight, low-cost stimulus/readout system is proposed, designed, fabricated, and tested for optogenetics study of neural activities. The system comprises a photodiode amplifier, LED driver, …
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Development and application of innovative technologies to unravel neuronal systems: From optogenetics to neural interfaces to spatial transcriptomics
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01
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Characterization of spontaneous and evoked activity of mouse embryonic stem cell derived motor neurons using optogenetics and micro electrode array electrophysiology
… array (MEA) electrophysiology and optogenetics to characterize electrical activity of MNs differentiated from mouse embryonic stem (mES) cells. A customizable substrate integrated 60-electrode MEA chip was designed for this study. MEA chips were fabricated with platinum deposited on …
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Strategies for optogenetic stimulation of deep tissue peripheral nerves
… in the central and peripheral nervous systems. Optogenetics refers to the transduction of mammalian cells with a light-sensitive transmembrane protein, called an opsin, such that illumination of the target tissue initiates depolarization; in the case of a neuron, illumination results in the …
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Accelerated Engineering of Optogenetic Tools with a High-throughput Microwell Array Platform
Optogenetics has shifted from a cutting-edge technology to a mainstay experimental technique. At its core, optogenetics is the use of light sensitive proteins to report or control biological activity. The unique spatiotemporal precision afforded by optogenetic tools has propelled the technique to …
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Optogenetic control of developmental signaling pathways
… in precisely defined spatiotemporal patterns. Optogenetics uses light-induced conformational changes to enable or disable protein-protein interactions, thereby permitting control of signal transduction at the flip of a switch. Consequently, light is emerging as a powerful tool to study …
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Informing the next generation of auditory midbrain implants, neuronal population dynamics in the auditory cortex and midbrain, and the potentials of optogenetic stimulation
… devices afford poor spatial resolution, which optogenetics may solve. Simultaneous silicon probe recordings were performed in the inferior colliculus (IC) and auditory cortex (AC) of awake, head-fixed mice, and repetitions of natural sound stimuli played. The two areas are different in their …
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Novel approaches to explore mechanisms of epileptic seizures - optogenetic and chemogenetic manipulation of hippocampal circuitry
… In the present thesis, we have applied optogenetics to study these questions, a technology based on modified microbial membrane channels or pumps that are introduced into target neurons, which can thereafter be activated by light. This is a powerful means of achieving excitatory or …
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The Regulation of Effort Exertion by the Nucleus Accumbens Core
… tools across many areas of neurobiology; but optogenetics has yet to be applied specifically to questions regarding the regulation of effort. Here these questions have been addressed by combining optogenetics (activation or inhibition of direct or indirect pathway neurons in the core or shell) …
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Multifunctional Wireless Gut-Brain Neurotechnology
… Cutting edge neurobiological tools like optogenetics, chemogenetics, and activity-based sensors have revolutionized studies of the brain. However, application of these methodologies for studies of brain-body circuits is reliant on engineered devices that support these sophisticated …
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Focusing Light Inside Scattering Media with Optical Phase Conjugation
… optical microscopy, optical tweezing, optogenetics, micro-surgery, and phototherapy.</p><p>To break the optical diffusion limit and focus light deep inside scattering media, optical phase conjugation based wavefront shaping techniques, such as time-reversed ultrasonically encoded (TRUE) …
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Odorant Responses and Courtship Behaviors Influenced by at4 Neurons in Drosophila
… analysis, single sensillum electrophysiology and optogenetics, I have examined the chemical tuning and behavioral consequences of the three at4 olfactory neuron classes. My results indicate that one class, Or65abc neurons, previously reported to be cVA sensors, are unresponsive to cVA pheromone. …
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