Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 147 for “"optogenetic"”.
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Optogenetic control of necroptosis pathway
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01
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Optogenetic control of developmental signaling pathways
… in precisely defined spatiotemporal patterns. Optogenetics uses light-induced conformational changes to enable or disable protein-protein interactions, thereby permitting control of signal transduction at the flip of a switch. Consequently, light is emerging as a powerful tool to study …
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Muscle Recruitment Mechanism under Optogenetic Neuromodulation
… its controllability. Recently, functional optogenetic stimulation (FOS) has demonstrated cell-type specificity and millisecond timescale neural control in the peripheral nervous system, enabling reduced fatigue and greater controllability when compared to FES. Given the novelty of FOS, no …
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Optogenetic examination of salt taste in mice
… salt appetite in mice. Experiment 3 showed that optogenetic stimulation of Type I taste receptor cells (TRCs) in transgenic mice could drive consumption of tap water under conditions of salt hunger. The fourth and final experiment assessed whether conditioned taste aversions to sodium would …
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Controlling Arrhythmias Using Optogenetic Actuators in Computational Simulations
… the application of light-induced stimulation (optogenetics) in computational models of heart cells (cardiomyocytes), cardiac tissues, and heart chambers to control arrhythmia development. Chapter 1 presents an overview of cardiac anatomy, relevant physiology, and cardiac electrophysiology …
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Opto-fluidic neural probes for optogenetic brain interrogation
… networks at any arbitrary stimulation volume. Optogenetics is an ideal tool for this purpose providing fast dynamics coupled with spatial precision typically brought in by genetic specificity. Several optogenetic tools have been developed in recent years to achieve spatial resolution by beam …
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Optogenetic regulation of protein activity in live cell
… study it’s role towards a specific cell fate. Optogenetic techniques utilize light to control protein functions with high spatial and temporal resolution. Here, I First present a generalizable light modulated protein stabilization system (GLIMPSe) that enables target-independent optogenetic …
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Independent two-color optogenetic excitation of neural populations
The optical modulation of neurons with channelrhodopsins, a class of genetically encoded light-gated ion channels, has enabled the spatiotemporally precise interrogation of the roles individual cell types play in neural circuit dynamics. A topic of great interest to the neuroscience community is …
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High-efficiency ultrasound-powered micro-LEDs for optogenetic applications
… opened a new opportunity for LEDs to be used for optogenetic applications. Optogenetics is an emerging field that combines genetic engineering and light for precise control or monitoring of biological functions of cells, neurons, or organs, and µLEDs (size ≤ 100 µm) are considered the best …
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Strategies for optogenetic stimulation of deep tissue peripheral nerves
Optogenetic technologies have been the subject of great excitement within the scientific community for their ability to demystify complex neurophysiological pathways in the central and peripheral nervous systems. Optogenetics refers to the transduction of mammalian cells with a light-sensitive …
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EFFECTS OF OPTOGENETIC ACTIVATION AND PHARMACOLOGICAL MODULATION OF DOPAMINE NEURONS
<p>This study explored the use of optogenetic tools to better understand treatments used for schizophrenia. The “positive” symptoms (e.g., hallucinations, paranoia, etc.) of schizophrenia may come from overexpression of dopamine in mesolimbic dopamine neurons. Positive symptoms can be produced in …
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Optogenetic dissection of the dopaminergic circuitry involved in memory consolidation
… model of everyday memory for mice and use the optogenetic toolbox to investigate the neuromodulatory circuitry that modulates persistence of everyday spatial memories. The task involved learning a win-stay rule with the daily goal of finding the location of food in the event arena. Using the …
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Optogenetic investigation of normal and pathological rhythms in the hippocampal formation
… be important for memory reconsolidation. Using optogenetic light ramp stimuli in transgenic mice, I found that gradual depolarization of CA1 pyramidal cells (mimicking CA3 sharp-wave input) is sufficient to induce fast gamma and sharp-wave ripple-like high frequency oscillations. I further …
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An Optogenetic Toolbox for the Interrogation of Dynamic Events During Angiogenesis
… tip cell development, we designed an optogenetic VEGFR2 utilizing the LightR regulatory system. We demonstrated that LightR regulation of RTKs provides spatiotemporal control over VEGFR2 signaling. Furthermore, we demonstrated that previous VVD modifications conserved their effect in …
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Accelerated Engineering of Optogenetic Tools with a High-throughput Microwell Array Platform
Optogenetics has shifted from a cutting-edge technology to a mainstay experimental technique. At its core, optogenetics is the use of light sensitive proteins to report or control biological activity. The unique spatiotemporal precision afforded by optogenetic tools has propelled the technique to …
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Optogenetic disruption of memory-drive, oculomotor behavior in the non-human primate
Pharmacological inactivation studies have shown that the frontal eye field (FEF) is critical for executing saccades to remembered locations. FEF neurons increase their firing rate during the three epochs of the memory-guided saccade task: visual stimulus presentation, the delay interval, and motor …
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Development of an Optogenetic Tool for Targeted Sensory Hair Cell Ablation in Zebrafish
Mechanosensory hair cells convert mechanical information into neuronal signals. Mutations in the genes required for hair cell function cause deafness and lead to the degeneration of sensory hair cells. Mammals are unable to regenerate these cells, leading to the permanent loss of hair cells. …
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Development of an Optogenetic Toolkit for the Interrogation of T cell Signalling Dynamics
… This thesis describes the development of new optogenetic approaches for the modulation of T-cell signalling dynamics and the interrogation of key events in T-cell activation to help investigate this question. Optogenetics is a rapidly emerging technique whereby light can be used to control the …
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