Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"optical sectioning"”.
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Single-Shot Optical Sectioning Using Polarised Illumination Coded Structured Illumination Microscopy (picoSIM)
… contrast.Removing out-of-focus light yields an optically sectioned image: a thin slice of a thick sample that only contains in-focus information. Taking a stack of such sectioned images allows for a three-dimensional (3D) view of the specimen. Structured illumination microscopy for sectioning …
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Applying single-molecule localisation microscopy to achieve virtual optical sectioning and study T-cell activation
… method for SMLM analysis is characterised: axial optical sectioning of SMLM images is demonstrated by thresholding fitted localisations using their fitted width and amplitude to reject fluorophores that emit from above or below a virtual ‘light-sheet’, a thin volume centred on the focal plane of …
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Nonlinear Microscopy System and Protocol for Rapid Evaluation of Freshly Excised Human Tissue
… protocol for histology requires physical sectioning of tissue, either with intense chemical processing and paraffin embedding or freezing of tissue, limiting the rapid evaluation applications. Nonlinear microscopy (NLM) is an emerging optical sectioning microscopy technology that enables …
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Methods for optical microscopy beyond conventional limits
Optical microscopy seeks to produce accurate and precise enlarged images of an object. It is highly desirable, particularly in live-cell biological microscopy, that the observation process does not perturb the structure, nor interfere with the dynamics, of the sample. These requirements are at odds …
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Miniature Microscope Design And Construction Based On Tilted Rotationally Asymmetric Printed Lenses
… (MEMS) actuator for optical sectioning using structured illumination. The opto-mechanical system is designed using a new concept called "zero alignment assembly" in which the lens elements snap into place and are constrained to a precise position with tolerances …
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Nonlinear microscopy for material characterization
… microscopy exhibits inherent 3- dimensional optical sectioning and provides access to previously unstudied aspects of materials. By probing third order nonlinear optical signals determined by the nonlinear susceptibility (chi-3), which is present in all materials, we gain insight not …
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Multi-view microscopy and multi-beam manipulation for high-resolution optical imaging
… in this thesis tackles some of the challenges in optical conventional and confocal microscopy and opens the third dimension for biological experiments like 3D cell cultures. Two instruments were built that provide high and isotropic resolution. In the Multi Imaging AxisMicroscope (MIAM) four …
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Theoretical and Experimental Concepts to Increase the Performance of Structured Illumination Microscopy
… SIM variants is introduced, the constraints of optical components for a flexible SIM system are discussed and the set-up is realised, the important aspect of deconvolution in SIM is highlighted and further developed, and finally novel SIM modalities introduced that improve its time-resolution, …
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Laser Scanning Imaging for Increased Depth-Of-Focus
… been proposed to improve the depth-of-focus in optical microscopy. Common techniques like optical sectioning microscopy and scanning confocal microscopy have innate problems. By simply modifying the pupil function in microscope imaging system, we can also extend the depth-of-focus. The scanning …
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Ultrafast optical pulse manipulation in three dimensional-resolved microscope imaging and microfabrication
… in two-photon excitation microscopy. First, optical fibers are routinely used in many optical instruments but their use in two-photon microscopy is very limited. As ultrafast light pulses propagate through conventional fiber optics, light pulses are dispersed and broadened, as a result of …
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Spectral-domain optical coherence phase microscopy for quantitative biological studies
… alterations. We have developed spectral-domain optical coherence phase microscopy (SD-OCPM) for highly sensitive quantitative phase imaging in 3D. This technique employs common-path spectral-domain optical coherence reflectometry to produce depth-resolved reflectance and quantitative phase …
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New technologies for optical coherence microscopy
… suffer from unacceptable false negative rates. Optical imaging technology that provides real-time, high-resolution imaging of human tissue in vivo with resolution at or near that of histopathology may significantly improve clinicians' capabilities to identify malignancies at curable stages. The …
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Large Two-photon Absorption of Highly Conjugated Porphyrin Arrays and Their in vivo Applications
… two-photon absorption which provides inherent optical sectioning capabilities. The NIR-II wavelength window (1000–1350 nm), a new biological imaging window, is promising for TPM because tissue components scatter and absorb less at longer wavelengths, resulting in deeper imaging depths and …
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Speckle applications for volumetric imaging of biological dynamics
… and this randomness is often treated as noise in optical imaging. In another perspective, the speckle statistics can be exploited to extract information that is not readily accessible through conventional imaging techniques. This thesis describes the application of speckle in assisting imaging …
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Techniques for high-speed high-resolution in-vivo volumetric fluorescence imaging
Optical microscopy has long been a cornerstone for exploring biological and biomedical systems. 3D microscopy with high spatialtemporal resolution over large fields of view is increasingly essential in life sciences. This thesis proposes two innovations to address this need: (1) high-resolution …
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Three-Dimensional Single-Molecule Localisation Microscopy and Visualisation Techniques
… to improve acquisition time, labelling density, optical sectioning and signal-to-noise ratio in comparison to 3D DH-PSF for biological imaging. In summary, this thesis shows the development of visualisation software (vLUME), improved depth of field imaging (SMLFM) and utilised DH-PSF imaging to …
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Spatial light interference microscopy and applications
… agents. However, the information about the optical thickness (or phase) is qualitatively mixed in the phase contrast intensity map. Quantifying optical path-length shifts across the specimen offers a new dimension to imaging, which reports on both the refractive index and thickness …
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Reconstruction Enhancements with Optical Scanning Holography
Optical scanning holography (OSH) [1] has the benefit of recording the entire three-dimensional (3-D) volume of a specimen in the form of a two-dimensional (2-D) hologram. Reconstruction of the original volume can be accomplished by applying digital reconstruction or decoding techniques to the …
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Identification of quantitative second-harmonic generation imaging metrics for collagenous tissue
… to collagen, sub-micrometer resolution, and optical sectioning capability. It is applied to various unstained collagenous specimens to explore the microstructure. Since collagen is one of the most abundant proteins in biological tissue, understanding its spatial organizational structure could …