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Showing 1 to 20 of 274 for “"oocytes"”.
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Vitrification of Bovine Oocytes
Cryopreservation of oocytes is a challenge. Studies were conducted to vitrify mouse zygotes and cumulus-intact bovine oocytes from follicles of different diameters, small (≤ 4 mm) and medium (4 to 10 mm), using nylon mesh. The specific goals were to assess changes in apoptotic gene expression …
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Advances in the fundamental cryobiology of mammalian oocytes
Having effective means to cryopreserve mammalian oocytes could increase the efficiency of managing populations of laboratory animals, increase the effectiveness of breeding programs for livestock, and improve the means by which assisted reproductive therapy is applied to human patients. …
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Organization of RNA Transcription Units in Amphibian Oocytes
Finally, the mechanism of hnRNP-G recruitment to the lampbrush loops is investigated. Interestingly, the RNA binding domain of the protein is not required for recruitment specificity to the loops. Instead, a poorly characterized portion of the C-terminus of the protein is required for this process.
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Factors influencing in vitro maturation of pig oocytes
… on in vitro maturation of pig oocytes. Cumulus oocyte complexes (COC) were obtained by aspiration of 2-5 mm follicles. The oocytes were cultured for 42 hours at 38.5$\sp\circ$C in mTCM-199 supplemented with LH, FSH, follicular fluid, fetal calf serum and estrous gilt serum, …
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EFFECT OF CRYOPROTECTANT EQUILIBRATION ON TRANSMEMBRANE POTENTIAL OF BOVINE OOCYTES
… on the transmembrane potential of bovine oocytes. The aim of this study was to evaluate and examine the difference in putative effects of non-permeating (PBS) and permeating (10% EG) solutions and to explore the association between osmotically induced volume changes on voltage in bovine …
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Investigating the regulation of processing bodies in Drosophila melanogaster oocytes
… mRNA, bicoid (bcd). In the mature oocytes, bcd is associated with P bodies and remains translationally silent. Upon egg activation, P bodies disassemble, releasing bcd into the cytoplasm where it associates with ribosomes and is translated. P body material properties are essential …
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Strategies to improve in vitro maturation of human and mouse oocytes
… (IVF) in 1978. In vitro maturation (IVM) of oocytes - a culture technique to support maturation of immature oocytes in vitro - could offer an interesting adjunct to the classical assisted reproductive treatments. From a clinical point of view, it could diminish or avoid the use of …
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Dynamics of Germ Cell Specific Stable Transcriptional Complexes in Xenopus Oocytes
… sequences to measure dwell time in living oocytes. I find that the dwell time of the factor proteins on the DNA or chromatin to which they specifically bind is much longer than is generally believed. This unexpected stability may contribute to the strength of cell differentiation in normal …
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The meiotic cell cycle and sister-chromatid cohesion in Drosophila oocytes
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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Profiling L-serine Transport Throughout Growth and Meiotic Maturation in Mouse Oocytes
… and understanding towards health requirements of oocytes and their inner workings are required. With current IVF success rates of approximately 40%, oocyte and embryo culture conditions in vitro can be improved by first understanding the finer details of oocyte function. As such, there is a need …
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In vitro maturation and fertilization of porcine oocytes and evaluation of male fertility
… (IVF), and utilizing these in vitro matured oocytes and in vitro fertilization techniques to determine the difference of sperm fertility from different boars. In the study of in vitro oocyte maturation, the oocyte maturation process was divided into the stages of germinal vesicle (GV I, GV …
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Transcriptional profiles of cumulus-oocyte complexes related to developmental competence in bovine oocytes
During folliculogenesis, oocytes and cumulus cells undergo many morphological and physiological changes. Transcriptome data were produced from single oocytes and corresponding cumulus cells (CC) to infer the differences in the transcript abundance from fully grown versus growing phase oocytes and …
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Calcium and actin coordinate egg activation and the metaphase-anaphase transition in Drosophila oocytes
Egg activation is the process through which the mature oocyte is prepared for embryogenesis, consisting of key cellular changes including, but not limited to: i) Physical and chemical changes to the oocyte’s outer covering; ii) The release of meiotic arrest, enabling the formation of a haploid …
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The role of transcription factors in somatic cell nuclear reprogramming by eggs and oocytes
… transcription factors during SCNR by Xenopus oocytes, which are inactive in DNA replication and cell division. QPCR measures changes in the steady-state levels of transcripts within 2 days of nuclear transfer to Xenopus oocytes (Oocyte-NT). Three pairs of Yamanaka factor homologs were tested …
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Structure-Function Studies on Human Epithelial Cystic Fibrosis Chloride Channels Expressed in Xenopus Laevis Oocytes
<p>The cystic fibrosis transmembrane conductance regulator (CFTR) Cl<sup>-</sup> channel is an ATP-binding cassette protein, comprising two transmembrane domains, two nucleotide binding domains (NBD1, NBD2) and a regulatory (R) domain. Channel gating is controlled by R-domain phosphorylation and by …
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Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
… to determine an efficient method of obtaining oocytes from cows via ultrasound-guided transvaginal follicular aspiration. Transvaginal oocyte recovery combined with in vitro maturation, fertilization, and culture (IVMlIVFIIVC) can produce pre-implantation stage bovine embryos and also supply a …
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Thermodynamics and kinetics of ice nucleation inside biological cells during freezing : as applied to mouse oocytes
Thesis (Ph. D.)--Harvard University--Massachusetts Institute of Technology Division of Health Sciences and Technology, Program in Medical Engineering and Medical Physics, 1989.
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Development of an automated microfluidic system for the loading and unloading of cryoprotectants from mammalian oocytes
Oocyte cryopreservation is an assistive reproductive procedure that has allowed women to overcome infertility and enjoy increased reproductive freedom. Despite a rapid increase in oocyte cryopreservation cycles over the past seven years, success rates both between and within clinics remain highly …
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Human oocytes and embryos viewed by time-lapse videography, and the development of an embryo deselection model
… the early biological variations between sibling oocytes, (ii) technical limitations in current equipment and protocols, and (iii) different insemination methods used (Papers 1&2). The above variations may be minimized by using pronuclear fading (PNF, a biological time point) as t0 rather than …
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