Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"oligonucleotide synthesis"”.
-
Synthesis and Reactivity of Electroactive Carbon Sulfide Ligands and Studies on Modifications of Nucleic Acids
… into the selective selenization of nucleic acid oligonucleotides was pursued. By using the soluble Se-transfer agent (i-PrC5H4)2TiSe5 in a new method with solid-phase phosphoramidite oligonucleotide synthesis, a mono-, di-, tri-, and tridecanucleotide of DNA with one phosphoroselenoate …
-
CRISPRI-based high-throughput functional genomic approaches for use in mycobacteria
… The first approach combines large-scale pooled oligonucleotide synthesis and nextgeneration sequencing, and is termed CRISPRi-Seq. A pooled library of 11 367 mutants, targeting 2 385 M. smegmatis genes with M. tuberculosis homologues, was constructed and used to infer gene essentialities which …
-
Synthesis and detection of dna damage
… at the cellular level due to their instability. Synthesis of oligonucleotides that contain a single N7-alkylguanine has become a vital tool to achieve the above goal. However, the instability of N7-alkyguanines is not compatible with the phosphoramidite chemistry used by solid-phase …
-
The Synthesis and Characterization of Glycol Nucleic Acids
… duplexes is the development of a straightforward synthesis of the phosphoramidites for solid phase oligonucleotide synthesis. Chapter 2 describes work towards a new set of exocyclic amino protection groups which could be removed in less time and under milder conditions than those previously …
-
Elucidating the mechanisms of action and evolutionary relationships of phage anti-defence proteins
… inhibit bacterial STING by producing competing oligonucleotides. Given that the eukaryotic cGAS–STING pathway senses viral DNA in the cytosol and activates immune responses via cyclic oligonucleotide synthesis, similar competitive mechanism could plausibly inhibit its activity in eukaryotes as …
-
Synthesis of internal amide bond short interfering RNAs (siRNAs) and investigation of their gene silencing properties
… due to the limitation of standard solid-phase oligonucleotide synthesis. In this thesis, we proposed to utilize phosphoramidite chemistry to localize internal amide-bond modifications [6]. A practical synthesis of a peptide nucleic acid unit combined with an RNA nucleoside (PNA-RNA dimer, UaU) …
-
DNA catalysts for amide bond cleavage and for lysine side chain modification
… that DNA can also form these structures. If oligonucleotides can act as comparable catalysts to proteins, a number of practical reasons to favor their development exist. The number of possible sequences is much smaller for DNA and RNA than for proteins; a sequence n units long has 4n possible …