Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"oligomeric structure"”.
-
Characterizing the oligomeric structure and catalytic activity of the dihydroorotase and aspartate transcarbamoylase from the bacterium, <i>bacillus anthracis</i>
<p>Bacteremia refers to bacterial presence in the blood. Bacterial proliferation in the blood requires that the organism adapt its metabolism to available nutrients. Nucleotide precursors that could be used are present at low levels in the blood, and thus the invading bacteria must rely on <em>de …
-
The establishment of the oligomeric purity and molecular structure for n-methylated bis(8-hydroxyquinoline) zinc (n = 0, 2, 4, and 5)
… material. However, no correlation of the structure of Znq2 to resulting device properties has been established. Most reports of Znq2 as an electroluminescent material have not considered the impact of higher order molecular structures on the device properties. By utilization of size …
-
Structure and dynamics of membrane proteins from solid-state NMR
… is an essential tool to elucidate the structure, dynamics, and function of biomolecules. This thesis mainly focuses on the structure determination of the hydrophobic domains for fusion proteins which are involved in membrane fusion between the cell membrane and viral envelope. Although …
-
Mapping protein-protein interactions in the <i>Escherichia coli</i> Twin Arginine Translocase
… peptides, with TatA being recruited to form an oligomeric structure that facilitates protein transport. Current models suggest that TatA oligomers locally disrupt membrane lipids allowing the substrate to move from the cytoplasm to the periplasm. Here, biochemical methods were used to further …
-
A COMPARATIVE ANALYSIS OF INTRACELLULAR CHOLESTEROL ESTERIFYING ENZYMES IN MAMMALS
… is non-covalent and does not alter the oligomeric structure of the protein. In the final project, the effect of protein stability on ACAT activity was investigated. Results showed that in vitro ACAT2 activity is always higher than ACAT1 probably due to high abundance of ACAT2 enzyme …
-
PLP-Dependent α-Oxoamine Synthases: Phylogenetic Analysis, Structural Plasticity, and Structure-Function Studies on 5-Aminolevulinate Synthase
… enzyme. Chimeric ALAS/AONS proteins exhibited an oligomeric structure with two sites having ALAS activity and two sites having AONS activity. Remarkably, the steady-state rates for both the ALAS and AONS activities were lower than that observed in the parent enzymes, while the reactivity of the …
-
Arginase from Bacillus caldovelox
… Mᵣ of 32000, suggesting a hexameric subunit structure. It has an isoelectric point of 5.4, a pH optimum for activity of 9 (at 60°C in 0.1M CAPS/NaOH buffer) and requires a divalent cation for activity. The purified enzyme contains 1.2 Mn²⁺ ions and 0.17 Fe²⁺ ions per subunit and is activated …
-
Purification and characterisation of plasmodium falciparum Hypoxanthine phosphoribosyltransferase
… filtration method for examining the quaternary structure of the protein and possible end stage purification, and initialcrystalization trials. a homology model of the open, unligaded PfHPRT is constructed using the atoomic structures of human, T.ccruz and STryphimurium HPRT as templates.
-
Studies on transient receptor potential vanilloid subtype 1 (TRPV1)-mediated degeneration of mesencephalic cellsin vivo and in vitro
… subtype 1 (TRPV1, also known as VR1) has an oligomeric structure formed by subunits having six transmembrane segments with a pore domain formed by the fifth and sixth transmembrane regions. This receptor is a nonselective cation channel activated by the vanilloids or products of …