Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"oligodeoxynucleotide"”.
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Esterase 2-oligodeoxynucleotide conjugates as enzyme reporter for electrochemical detection of DNA and identification of bacterial species
… and covalently conjugated to a 5’-amino modified oligodeoxynucleotide. The purified conjugate served as a reporter enzyme for electrochemical detection of nucleic acids. Being an optimal substrate, p-aminophenylbutyrate exerts maximal signal response to EST2 in E-Chip, as determined by comparison …
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Glial Changes and Role of Connexin43 in a Laser-induced Rodent Model of Retrobulbar Optic europathy
… points after the injury. Connexin43 Antisense Oligodeoxynucleotide has been investigated as a potential neuroprotective agent following the optic nerve injury. Results: Glial changes have been documented following laser-induced injury to the optic nerve. No optic nerve head swelling has been …
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Isolation And Characterization Of Human Ribosomal Protein Complementary-dnas
… method of isolation employed mixed sequence oligodeoxynucleotide probes to identify human ribosomal protein S6 cDNAs. Ribosomal protein S6 is the major substrate of protein kinases in eukaryotic ribosomes. Published amino acid sequence data for rat liver ribosomal protein S6 and yeast …
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CNS delivery of antisense oligodeoxynucleotides using biodegradable microspheres
Antisense oligodeoxynucleotides can selectively inhibit gene expression provided they are delivered to their target site successfully for a sufficient duration. Biodegradable microspheres have previously been developed for the potential systemic delivery of antisense oligodeoxynucleotides and offer …
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A study of DNA-DNA interactions during bacteriophage lambda integrative recombination
… recombination in bacteriophage lambda. Oligodeoxynucleotides 28 bases long containing either the top strand or the bottom strand sequences of the bacterial attachment site (attB) with or without base substitutions in the core region were chemically synthesized. Annealed …
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Development of an analytical method to quantify the oxidative deoxyribose damage product 3'-phosphoglycolaldehyde induced by radiation, iron and peroxynitrite
… by analysis of a synthetic, PGA-containing oligodeoxynucleotide. The limit of detection in the presence of DNA is 30 fmol of PGA. The analytical method was then applied to the quantification of PGA in purified DNA and cultured cells treated with several oxidants.
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Evaluating The Therapeutic Efficacy of Grb2 Inhibition In Ovarian Malignancies
… the biological effects of liposomal antisense oligodeoxynucleotide that blocks Grb2 expression (L-Grb2) in ovarian cancer models.</p> <p><strong>Experimental Design: </strong>Murine orthotopic models of ovarian cancer (OVCAR5 and SKOV3ip1) were used to study the biological effects of L-Grb2 on …
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Kupplung der DNA-Methyltransferase M.SssI mit Triplehelix-bildenden Oligodesoxynucleotiden
… is to couple them with triple helix forming oligodeoxynucleotide (TFO). These additional DNA recognition units should address the enzymes only to predetermined DNA recognition sequences and result in an increase in their sequence specificity. In this research, the DNA cytosin-C5-MTase M.SssI …
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Cloning and characterization of the 3' terminal regions of RNA from select strains of maize dwarf mosaic virus and sugarcane mosaic virus
… coat proteins of these viruses. Additionally, oligodeoxynucleotide directed site specific mutagenesis alteration of the MDMV-B cDNA clone allows excision of the NI$\sb{\rm II}$ coding region and introduction of a translation start site for the (MDMV-B) coat protein. Subsequent in vitro …
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Enhancement of Protective Immune Responses in Chickens against Marek's Disease Virus
… (LPS) as a TLR4 agonist and CpG oligodeoxynucleotide (ODN) as a TLR21 agonist and infected with the RB1B strain of MDV via the respiratory route. The results of this study indicated that treatment of MDV-infected chickens with TLR4 and 21 agonists induced the expression of type I …
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Neurochemical Changes Following Stroke: Role In Neuroprotection And Cardiac Susceptibility
… to stroke was investigated using antisense oligodeoxynucleotide specific for NPY-Y1 receptors. A reduction in Y1 binding sites within the IC produced a doubling of the infarctolume following MCAO.;The possibility that these neurochemical changes mediate cardiovascular complications of stroke …
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Surface Engineering of Protein Nanoparticles for Intranasal Delivery
… coating of trimethyl chitosan and CpG oligodeoxynucleotide adjuvants delivered intranasally in murine models and compared to unmodified protein nanoparticles. The localization and biodistribution were observed using non-invasive in vivo imaging and for regional localization and tissues …
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Biodegradable polymers for the sustained release of antisense nucleic acids
Antisense oligodeoxynucleotides can selectively inhibit individual gene expression provided they remain stable at the target site for a sufficient period of time. Thus, the efficacy of antisense oligodeoxynucleotides may be improved by employing a sustained release delivery system which would …
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LOCOREGIONAL DELIVERY OF UNMETHYLATED CPG-OLIGODEOXYNUCLEOTIDES TO CANCER THERAPY: PRECLINICAL STUDIES
… Toll-like receptor-9 (TLR9) agonist CpG-oligodeoxynucleotide (CpG-ODN) oppositely modulates expression of DNA repair genes in tumor and immune cells. Analyses in silico and in a human ovarian tumor model by microarray revealed downregulation of these genes in tumors and upregulation in …
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Interaction of peptides, peptidomimetics and other drug candidates with the DI/tripeptide transport system in intestinal epithelial cells, using the in vitro caco-2 cell culture system
… the cell. This was also shown to be the case for oligodeoxynucleotide conjugated to a lipophilic group (vitamin E), and highlights the possibility that other orally administered drug candidates may exert non-specific effects on the DTS and possibly have a nutritional impact. Molecular modelling of …
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Super-specific DNA methylation by a DNA methyltransferase coupled with a triple helix-forming oligonucleotide
… one cysteine residue, has been coupled with an oligodeoxynucleotide (ODN) also by heterobifunctional crosslinking, but in a longer synthetic route featuring the temporary protection of the active site cysteine (C141). Coupling with catalytic cysteine could lead to a loss of DNA MTase activity. …