Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"nude mouse"”.
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VOLUMETRIC GROWTH MODEL OF HUMAN MEDULLOBLASTOMA IN THE NUDE MOUSE CEREBELLUM
… a human medulloblastoma (VC312) in the athymic nude mouse cerebellum using Gd- enhanced T1-weighed MRI scans. Additionally, a medulloblastoma flank tumor model was used to explore the in vivo effect of the oral anti-cancer agent that inhibits Akt activation in the phosphoinositide 3-kinase …
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Application of Cell-Derived Extracellular Matrix (ECM) Scaffold on Tissue Engineering Cartilage
… tissue engineering in vitro and in vivo as nude mouse and rabbit model. Chapter I: A porous cell-derived ECM scaffold was prepared with a freeze-drying protocol using porcine chondrocytes. The ECM scaffold had highly uniform porous microstructure by scanning electron microscope (SEM). It …
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MICRO-CT EVALUATION OF MSC/HUVEC/HSC-MEDIATED ANGIOGENESIS IN LARGE PCL-ßTCP CONSTRUCTS IMPLANTED IN NUDE MICE
… hybrid scaffold in an immuno-compromised nude mouse. The PCL-ßTCP scaffold (30% PCL: 70% ßTCP; 70% porosity) with interconnected open pores was fabricated in 6x6x6mm cubes. They were seeded with human mesenchymal stem cells (MSCs) plus HUVECs or HSCs. Groups (N=12) consisted of scaffold …
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Magnetic Resonance Imaging of a Natural Killer Cell Therapy in a Mouse Model of Prostate Cancer
… to study natural killer (NK) cell therapy in a nude mouse model of prostate cancer. Cellular MRI, anatomical MRI and hyperpolarized 13C spectroscopy were used to study various aspects of the model. Methods: The cells used were KHYG-1 NK cells and PC-3M prostate cancer cells. Imaging was …
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Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas
… deficiency (LSCD) was established in athymic nude mice (n=75) using ethylenediaminetetraacetic acid (EDTA) and mitomycin C treatment followed by mechanical debridement of corneal and limbal epithelium. Immunofluorescence (IF) using Anti-Laminin and Propidium iodide (PI) was used to assess …
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Somatic cell gene therapy for diabetes mellitus: engineering a surrogate B-cell
… β-cells. Initial studies were conducted using mouse pituitary AtT20 cells as a model, since these cells possess an endogenous complement of enzymes capable of processing proinsulin to mature insulin. Glucose sensitive insulin secretion was conferred to these cells by transfection with plasmids …
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Proteolytic mechanisms involved in the metastasis of human melanoma cells
… has been shown to be highly metastatic in the nude mouse. This cell line is typical of many melanomas in that it synthesizes only tPA and not uPA. In part 1 of this thesis I further investigated the plasminogen activator production by these cells (at the level of mRNA as well as activity) as …
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Effect of Entinostat On Nk Cell-Mediated Cytotoxicity Against Os Cells and Os Lung Metastsis
… of entinostat. We elected to use our nude mouse human OS lung metastasis model for this purpose. <em>In vitro</em>, entinostat increased NK cell ligands on OS cells (MIC A/B, ULBP1, ULBP2/5/6, and CD155) and enhanced the NK cell-mediated cytotoxicity. Entinostat oral administration …
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The Quantitative Assessment of Osteoinductivity of Human Demineralized Bone Matrix and cDNA Array Analysis of Osteogenic Differentiation in Human Periosteal Cells
… bone formation in an <em>in vivo</em> athymic mouse bioassay. DBM exhibiting high osteoinductivity in the nude mouse bioassay contained higher amounts of extractable BMP-4 than did DBM samples possessing low osteoinductivity.</p> <p>In the study of the effect of residual calcium on …
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Immunohistochemical analysis of a panel of human and murine markers on xenografted human vaginal mucosa: a comparative study
Athymic nude mouse models have been extensively used to study biological behaviour of normal and diseased human tissues. In such models, immune-deficient mice act as hosts for cysts constructed from human material. A unique biocyst model that entails transplantation of human vaginal cysts into …
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Approaches for Enhancing Therapeutic Efficacy of a Novel IL-10 Gene Family Member: MDA-7/IL-24
… by in vivo human xenograft studies in nude mice, mda-7/IL-24 (administered as Ad.mda-7) was evaluated in a Phase I clinical trial in patients with melanomas and solid cancers. These studies document that mda-7/IL-24 is well tolerated and demonstrates evidence of significant (44%) …
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Functional analysis of N-MYC downstream regulated gene 1 (NDRG1) in Oesophageal squamous cell carcinoma
… as measured in cell culture. Accordingly, in the nude mouse xenograft model system, NDRG1 overexpression promoted the in vivo growth and metastasis of KYSE30 derived xenografts, which could be attributed to the reduced apoptotic and enhanced angiogenic activities promoted by this gene. …
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Expression and function of osteopontin variants in HCV-related liver disease and hepatocellular carcinoma.
… –C) accelerated Huh-7 derived tumor growth in a nude mouse model. We also show for the first time expression of all three OPN variants in the non-diseased liver as it was previously thought that splicing was a feature specific for tumor cells. Clinically, OPN is known to be highly expressed in …
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The anticancer potential of CITme, a quinoline derivative
… activity of CITme could not be determined in a nude mouse tumour model, using the most susceptible in vitro cell line, DU-145 due to the low success rate of tumour induction and the long lag period required for the development of subcutaneous prostate tumours after transplantation of either a …
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An experimental study of human melanoma cells cutured in vitro
… with in vivo behaviour as measured in the nude mouse. Melanoma cell lines were established from biopsy material obtained from 7 patients at Groote Schuur Hospital. Two of these lines synthesized tyrosinase and melanin at a rate that was directly related to cell density. The five remaining …
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Investigating the nuclear import protein KPn߀1 as a cancer therapeutic target
… allowing for its testing in a xenograft nude mouse model. Intraperitoneal injection of C60 selectively inhibited the growth of oesophageal tumour cells with a significant effect observed on KYSE 30 oesophageal tumours. To investigate Kpnβ1:C60 binding interactions, Kpnβ1 was purified …
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Wundheilung athymischer Nacktmäuse nach Transplantation von kultivierten humanen Keratinozyten und Fibroblasten auf PLGA-Microsphären als Kokultur in Fibrinklebermatrix
Hauttransplantation von zuvor kultivierten humanen Keratinozyten und Fibroblasten als PLGA-Microcarrier-Kokultur in einer Fibrinklebermatrix auf eine standardisierte dorsale Vollhautwunde der athymischen Nacktmaus. Makroskopische und Histologische Untersuchung der sich ausbildenden Neoepidermis und …
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Resveratrol Analogs: Potential Chemopreventive Agents in Breast Cancer
… reduce primary tumor growth of xenografts in a nude mouse model. But its clinical applications in prevention of breast cancer are limited because of its lower efficacy in in vivo systems. Thus, to improve the anticancer and antioxidant efficacy of Res and to use it as a successful agent …
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Hepatotoxische Nebenwirkungen adenoviraler Genvektoren erster Generation
Hepatotoxische Nebenwirkungen adenoviraler Genvektoren erster Generation <br> <br> <br>Beim systemischen Einsatz adenoviraler Genvektoren sind bislang eine Vielzahl toxischer Effekte im Organismus beobachtet worden. Eine genaue Kenntnis dieser Nebenwirkungen ist deshalb sowohl für die Evaluierung …