Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 113 for “"nucleotide binding"”.
-
Diphosphopyridine Nucleotide Binding by Apodehydrogenases
Made available in DSpace on 2014-12-05T19:37:17Z (GMT). No. of bitstreams: 1 6000199.pdf: 3037417 bytes, checksum: 6d6ee9972aa702d714e7ffab685be9a1 (MD5) Previous issue date: 1959
-
The nucleotide binding domains of multidrug resistance-p-glycoproteins
Bibliography: leaves 116-128.
-
Control of HslUV protease function by nucleotide binding and hydrolysis
… act as molecular machines, using the power of nucleotide binding and hydrolysis to drive conformational changes in themselves and their target substrates. Like other AAA+ proteases, HslUV recognizes, unfolds, translocates, and degrades substrate proteins in an ATP-dependent manner. …
-
Partial Characterization of Adenine Nucleotide Binding to Isolated Chloroplast Coupling Factor
Made available in DSpace on 2014-12-14T05:40:43Z (GMT). No. of bitstreams: 1 7726773.pdf: 4907099 bytes, checksum: 53a8598ff0c34fba7539faea61903d9c (MD5) Previous issue date: 1977
-
Fluorescence Studies of Dihydrodiphosphopyridine Nucleotide Binding by Rat Liver Lactic Dehydrogenase
Made available in DSpace on 2014-12-05T19:37:28Z (GMT). No. of bitstreams: 1 6003933.pdf: 4519576 bytes, checksum: bc2a0694c3ca81ef46d55643f5fc828a (MD5) Previous issue date: 1960
-
Properties of the nucleotide binding sites of the Ca²⁺-ATPase of sarcoplasmic reticulum
Properties of the nucleotide binding site of the Ca²⁺-ATPase of skeletal muscle sarcoplasmic reticulum have been investigated. The study centred around interaction of the high affinity ATP analog, 2'-3'-0-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate, (TNP-ATP), with the Ca²⁺-ATPase. Defined …
-
Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine
… a AAA+ ring homohexamer, uses the energy of ATP binding and hydrolysis to power conformational changes that unfold and translocate target proteins into the ClpP peptidase for degradation. X-ray crystal structures show that some ClpX subunits occupy nucleotide-loadable conformations and others …
-
X-ray crystallographic studies of the nucleotide binding sites on adenylosuccinate synthetase mutants from Escherichia coli
… only water molecules in the putative aspartate binding site. As a consequence the 300's loop is disordered in the R3 03 L structure. In the K 16Q structure, NE2 of Gln 16 is in approximately the same position as NZ of Lys 16 of the wild-type enzyme. The void created by the elimination of the …
-
Kinetics and specificity of nicotinamide nucleotide binding to the dIII component of transhydrogenase from Rhodospirillum Rubrum
… is thought to function by way of a binding-change mechanism, which involves “open” and “occluded” conformations of the enzyme. In the open conformation, nucleotides can readily bind and dissociate from the enzyme but the hydride transfer reaction is blocked. In the occluded …
-
Allosteric Determinants of Guanine Nucleotide Binding Proteins and Methods to Crystallize the Cytosolic Domains of Adenylyl Cyclase
… Members of the G protein superfamily contain nucleotide-dependent switches that dictate the specificity of their interactions with binding partners. Using a new sequence-based method termed statistical coupling analysis (SCA), I identified the allosteric core of these proteins - the network of …
-
MOLECULAR CHARACTERIZATION OF THE PUTATIVE NUCLEOTIDE- BINDING AND HYDROLYZING ACTIVITIES OF THE ELLIS VAN CREVELD (EVC) PROTEIN
… and nuclear localization signal. The putative nucleotide-binding and hydrolyzing activities of EVC were tested using fragments of the protein from mice and human that contain the P-loop and leucine zipper regions. The presence of GTP analog caused the quenching of the intrinsic fluorescence of …
-
Characterization of the Reaction Cycle of MJ0796: A Model Archaeal Adenosine Triphosphate-Binding Cassette Transporter Nucleotide Binding Domain
Adenosine-triphosphate binding cassette (ABC) transporters couple nucleotide hydrolysis to vectorial transport of solutes across lipid bilayers. These proteins, found in all kingdoms of life, have been implicated in a variety of human genetic disorders and engender drug resistance in cancer cells …
-
Rhodopsin kinase structure: different nucleotide-binding states and implications for mechanism of activation of a G protein coupled receptor kinase
… six crystal forms that represent three distinct nucleotide-ligand binding states. One of the (Mg²⁺)₂·ADP·GRK1 structures is the most high-resolution structure (1.85 Å) of a GRK to date. In one (Mg²⁺)₂·ATP·GRK1 structure, almost the entire N-terminal region (residues 5-30) is observed. In …
-
Receptor- G Protein Interactions in the Visual System: A Study of Structures and Mechanisms That Couple the Cytoplasmic Surface of Rhodopsin to the Nucleotide-Binding Pocket of Transducin
… of the R*-Gt complex and the mechanism of nucleotide exchange are unknown. We studied the function of the fourth cytoplasmic loop (C4) of rhodopsin in interactions with Gt. Chimeric mutants of rhodopsin were characterized in which regions of C4 were replaced with amino acid sequences from …
-
Characterization of two genes, trehalose-6-phosphate synthase/phosphatase and nucleotide binding protein, shown to be differentially regulated in roots of Cypripedium parviflorum var. pubescens grown with a mycorrhizal fungus Thanatephorus pennatus
… (Tps), which showed down-regulation and nucleotide binding protein (NuBP), which showed up-regulation. The Tps partial clone identifies 2100 bp at the 3' end of the gene and encodes a protein of 667 amino acids. The NuBP gene is approximately 1200bp in length and encodes a protein of 352 …
-
Structural and Functional Studies of ASCC1 in DNA and RNA Alkylation Damage Response
… combines two evolutionarily ancient domains: nucleotide-binding K-Homology (KH) (associated with splicing, transcriptional regulation, and translational control) and two-histidine phosphodiesterase (PDE) (associated with hydrolysis of cyclic nucleotide phosphate bonds). Germline mutations link …
-
Regulation of Guanine Nucelotide Exchange in Inhibitory G Protein Alpha Subunit by Activator of G Protein Signaling 3 and Novel Regulatory Peptides
The release of GDP from the nucleotide binding pocket of G protein a subunit (Ga) is accelerated by guanine nucleotide exchange factors (GEFs) and inhibited by guanine nucleotide dissociation inhibitors (GDIs). The beta gamma subunit of heterotrimeric G protein and GoLoco motif are GDIs. Activator …
-
Gene cloning, adjustable expression and site-directed mutagenesis of Escherichia coli adenylate kinase
… level did not have any effect on the adenine nucleotide concentrations. Site-directed mutagenesis was used to investigate the location of the ATP and AMP binding sites on adenylate kinase and the mechanism of substrate inhibition. Two amino acids, phe86 and tyr133, which were found to be in …
-
Actin-like filament A (AlfA) and AlfA-driven plasmid segregation
… in bacteria that can form filaments upon nucleotide binding and their involvement in various cellular processes including cell shape determination, cell division, and DNA segregation led to a reconsideration of the evolutionary history of life. Actin-like filament A (AlfA) is a bacterial …
-
Structure and regulatory mechanism of human uncoupling protein 1
… of UCP1 are regulated by cytosolic purine nucleotides, which inhibit proton conductance and therefore enable activation only in response to cold environments. This thesis aimed to determine the first high-resolution structure of UCP1 to address several unanswered questions about its overall …
Page 1 of 6