Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"nucleosome assembly"”.
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Hepatitis C Virus NS5A Targets the Nucleosome Assembly Protein NAP1L1 to Control the Interferon Response
… factors involved in promoting viral replication/assembly and in the cell antiviral response to HCV. Interaction of NS5A with the nucleosome assembly protein NAP1L1 has been recently characterized in my laboratory. NAP1L1 is a histone chaperone protein with various functions related to nuclear …
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ANALYSIS OF HUMAN DNA MISMATCH REPAIR IN THE CHROMATIN ENVIRONMENT
… The first step in chromatin establishment is nucleosome assembly, that starts with histone tetramer deposition. It is not clear how three important cellular processes: genome replication, mismatch repair and nucleosome assembly are coordinated. Here we analyzed human mismatch repair in the …
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Regulatory network built by Cytochrome c with Histone Chaperones in response to DNA damage
… altering chromatin dynamics through their nucleosome assembly activity. Recent studies have reported that the mitochondrial respiratory protein cytochrome c is promptly translocated into the cell nucleus upon DNA damage, where it interacts with several histone chaperones. Within this …
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Identification and Characterization of Hells-CDCA7, a Nucleosome Remodeling Complex Required for Mitotic Chromosome Structure
… I show that nearly all proteins involved in nucleosome assembly and remodeling are evicted from mitotic chromosomes, concurrent with a reduction in mitotic nucleosome assembly and discuss a role for these phenomena in shaping mitotic chromosomes. In analyzing my mass spectrometry data, I …
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The structural analysis of histone H3 lysine 56 acetylation and related histone chaperone complexes
… eukaryotic organisms is regulated in part by the assembly and disassembly of chromatin. Key regulators of these processes are histone binding proteins known as histone chaperones. The histone chaperone complex Chromatin Assembly Factor 1 (CAF-1) is responsible for depositing H3.1/H4 tetramers in a …
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Antigenic Variation and Telomere Structure in Trypanosoma brucei
… TTAGGG binding activity and by the trypanosome nucleosome assembly protein NAP1.</p>
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Development of a TIRF Microscope for Single Molecule Binding Studies
… between PCNA and CAF-1 in replication-coupled nucleosome assembly to contribute to epigenetic inheritance. The microscope measures fluorescence following total internal reflection (TIR) to accomplish single molecule imaging (SMI). TIR provides a high signal-to-noise ratio (SNR) by generating a …
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Mutational analysis of the human histone chaperone, Nap1, in nucleosome disassembly at the HTLV-1 promoter
… of condensed chromatin, alteration of nucleosome structure, and partial or complete disassembly of the histone octamer. Our laboratory uses human T-cell leukemia virus, type 1 (HTLV-1) as a model for studying eukaryotic transcription activation and gene regulation. Previous studies …
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Regulation of Sister-Chromatid Cohesion
… interfering Okazaki fragment processing and nucleosome assembly during DNA replication do not impact interphase cohesion, suggesting that cohesion establishment occurs before Okazaki fragment maturation and histone deposition. Our results demonstrate that DNA replication-coupled cohesin …
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Responses to Antifungal and Alkaline pH Stress in Human Fungal Pathogens
… signaling partners or interactors, we identified nucleosome assembly protein 1 (Nap1) as an interactor of the Rra1 pH sensor. Like Rra1, C. neoformans Nap1 is required for the activation of the Rim pathway. Nap1 specifically interacts with the Rra1 protein, acting as a scaffold to maintain …
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THE INTERPLAY OF HISTONE CHAPERONE NAP1 WITH SWI/SNF FAMILY OF CHROMATIN REMODELLERS IN S.cerevisiae
Histone disassembly is one of the mechanisms by which the ATP dependent chromatin remodelers remodel the nucleosomes. However nucleosome remodeling is also regulated by other factors like covalent modifications marks (methylation, acetylation etc. of both histones and chromatin remodelers). and …
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Making Molecular Movies: Using Single-Molecule Techniques to Unveil Hidden Features of Protein-Chromatin Interactions
… genomic DNA is also packaged into units called nucleosomes, which offer another layer of physical regulation for proteins operating on chromatin. Previously, tracking these protein behaviors on DNA and chromatin had been technically challenging, dependent on tools that could access short-lived, …