Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"nucleoprotein"”.
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CHARACTERIZATION OF INFLUENZA NUCLEOPROTEIN BODY DOMAIN AS ANTIVIRAL TARGET
… virus. Each RNA segment is encapsulated by viral nucleoprotein (NP) and bound by the viral RNA dependent RNA polymerase (RdRP) to form viral ribonucleoproteins (vRNPs) responsible for RNA synthesis. NP is a structural component of the vRNP but also interacts with both viral and host factors to …
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Identification of novel cellular components associated with early HIV-1 nucleoprotein complexes
… proteins in HIV-1 replication. The viral nucleoprotein complexes (NPCs) formed during early HIV-1 infection represent potential targets for antiviral therapy. Despite extensive research the structure and composition of these structures remain poorly characterized. To better characterize …
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Comparison of nucleoproteins and nucleic acids of five isolates of tomato ringspot virus
… could be differentiated into three strains by nucleoprotein and nucleic acid analysis. A grape isolate from one location (GSGF), two grape isolates from another location (GBNR, G4NR), a seed-borne soybean isolate (S1B), and a dandelion isolate (D4WR) from the second location were compared. The …
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Cooperative Assembly of Terminase and Integration Host Factor at the Packaging Initiation Site of Bacteriophage Lambda
… at cos and wrap the duplex into a compact nucleoprotein complex. Rigorous analysis of this cooperative assembly is complex due to the multiple terminase and IHF binding elements within cos. Therefore, I dissected the cos site into individual specific and nonspecific IHF binding sequences, …
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Purification of Human Immunodeficiency Virus Type 1 Preintegration Complexes by Velocity Gradient Centrifugation.
… 1 (HIV-1) infection are mediated by two large nucleoprotein complexes (NPCs), the reverse transcription complex (RTC) and the preintegration complex (PIC). These complexes are responsible for the conversion of viral RNA into viral DNA, transport through the cytoplasm, import into the nucleus, …
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Pam2ODN-Induced Epithelial Resistance Against Influenza A Requires STAT3 Activation
… We performed quantitative-PCR probing for Iav nucleoprotein (NP) and observed that cells with STAT3 inhibition and Pam2ODN treatment had elevated IAV nucleoprotein (NP) burden compared to cells treated with Pam2ODN only. Furthermore, we challenged lung epithelium-specific STAT3 deletant mice …
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Increased sensitivity for lateral flow immunoassays through signal amplification methods
… for detection of disease targets (e.g. nucleoprotein of the influenza virus). However, the use of these LFTs is limited because of their well-recognized lack of sensitivity. To improve the sensitivity of LFTs for influenza diagnosis while maintaining a simple and rapid assay procedure, …
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Biochemical and towards structural characterization of RAD51 and RAD51AP1 complexes
… promotes HRR by assembling onto ssDNA to form nucleoprotein filaments and catalyses key HRR steps of strand invasion and homology search in the sister chromatid. A large number of accessory factors and RAD51 binding proteins, with inherited mutations in many of these predisposing to cancer, …
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Novel Structures of RAD51 Reveal Mechanisms in DNA Damage Repair and Genomic Stability
… I present three-dimensional structures of RAD51 nucleoprotein filaments together with biochemical and biophysical data that reveal new insights into how RAD51 contributes to maintaining the stability of our genome. High-resolution structures of RAD51 filaments on single- (ss-) and double-stranded …
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Universal room-temperature stable influenza A nanovaccine
… composed of a novel equine IAV antigen and IAV nucleoprotein delivered via polyanhydride nanoparticles and pentablock copolymer micelles. This rationally designed subunit vaccine formulation was evaluated to determine both the release kinetics and the functional stability of antigenic loadings. …
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Chaperoning viral protein evolution
… profile the mutational tolerance of influenza nucleoprotein and hemagglutinin in modulated cytosolic and endoplasmic reticulum (ER) folding environments, respectively. The nucleoprotein work provides the first experimental evidence that host chaperones can enhance the accessibility of …
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Proviral Roles of Ebola virus VP35 Ubiquitination
… enhanced interaction with the viral nucleoprotein and may trigger premature nucleocapsid packaging. Prior work also showed that at least one lysine residue other than K309 is ubiquitinated and that VP35 non-covalently interacts with ubiquitin. We observed that multiple residues in …
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
… characterize the thermodynamics and kinetics of nucleoprotein complex formation. Mechanical pulling of ssDNA in the presence of SSB reveals that the protein condenses ssDNA in the force range 0-10pN and that tension can be used to modulate the ssDNA wrapping state of SSB. Measurements of SSB …
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The amino acid sequence of chicken histone F3
… was isolated by selective extraction from nucleoprotein with ethanolic-HCl and purified by a single gel filtration step. This protein was found to be homogeneous by the following criteria: gel filtration, electrophoretic mobility, N- and C-terminal amino acid residues and amino acid …
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Polysequence Nanomaterials for Immunomodulation
… epitopes PADRE and a conserved segment of the nucleoprotein of influenza. Likewise, when combined with the influenza surface protein hemagglutinin, KEYA modifications strengthened the resulting influenza-specific cellular immune responses. Augmented immune responses typically followed native …
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Investigation of Vesicular Stomatitis Virus Interaction with Host Cell Proteins and Associated mRNA Structures
… RNA-binding proteins, heterogeneous nuclear ribonucleoproteins (hnRNPs), relocalized from the nucleus to the cytoplasm during infection with VSV. We performed co-immunoprecipitation and RNA-seq experiments to identify and quantify the RNAs that interacted with hnRNP A1, hnRNP C1/C2, and hnRNP K …
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Analysis of the Nucleioprotein Complexes Essential for P1 Plasmid Partition
… The goal of this thesis was to investigate the nucleoprotein complexes essential in the P1 plasmid partition reaction. First, I examined how a single ParB dimer can bind its complicated arrangement of recognition motifs in parS to initiate the partition reaction. I then characterized a novel …
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Genetic and mechanistic studies of the bacteriophage lambda integrase protein
… starts with the formation of higher-order nucleoprotein complexes, called ""intasomes"", followed by a series of steps catalyzed by the phage-encoded integrase (Int). In order to study the functions of Int during recombination, Int mutants defective in in vivo excision were isolated and …
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INFECTION- AND VACCINE-INDUCED IMMUNE RESPONSES TO SARS-COV-2 AND OTHER HUMAN CORONAVIRUSES
… T cell responses were variable between groups. A nucleoprotein mRNA vaccine study in mice demonstrated different patterns of T cell and antibody cross-reactivity between human Embecoviruses with significant nucleoprotein-specific T cell cross-reactivity between the human common cold coronaviruses, …
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