Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"nucleoporins"”.
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Structural studies of large architectural nucleoporins and coat proteins
… envelope. It assembles from ~30 proteins, termed nucleoporins or nups, symmetrically arranged about a central 8-fold axis. Some nucleoporins also contribute to other structures or perform diverse other functions. A subset forms a stably-associated core scaffold for the NPC, organized into two …
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A nanobody suite for yeast scaffold nucleoporins provides details of the Y complex structure and nuclear pore complex assembly
… assembly of ~500 individual proteins, called nucleoporins or nups, that can be classified into three categories: 1. Stably associated scaffolding nups, 2. Peripheral nups, and 3. Phenylalanine-glycine (FG) repeat containing nups that form the permeability barrier of the NPC. Most scaffolding …
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Studies on the interaction of HSV-1 multifunctional protein ICP27 with the nuclear pore complex proteins
… pore complex, which consists of proteins called nucleoporins (NUPs). NUPs act to regulate the transport cargos by interaction with mRNA bound proteins and transport receptors, karyopherins. To further explore the transport requirements for ICP27 and viral mRNA export, we investigated its direct …
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Characterisation of ALADIN’s function during cell division
… of the nuclear pore complex (NPC) also called nucleoporins. NPCs are large complexes studded in the nuclear envelope, which control the nucleocytoplasmic transport. It is now known that nucleoporins participate in spindle assembly, kinetochore organisation, spindle assembly checkpoint, and all …
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Structural and biochemical analysis of the Y-shaped Nup84 subcomplex of the NPC
… Overall, the NPC is composed of -30 proteins, or nucleoporins (nups), that form biochemically stable subcomplexes which are repeated in multiple copies to assemble the intact NPC. This macromolecular machine is not only essential to nucleo-cytoplasmic transport, but also plays a pivotal role in a …
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Mechanism and Regulation of ERK2 Subcellular Localization
… transport factors, but requires access to nucleoporins. While this process is not influenced by classical import machinery, it can be modulated by anchoring proteins that bind to ERK2 and sequester the kinase in the cytoplasm. One of these proteins, PEA-15, prevents ERK2 import in an in …
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Conformational Dynamics of the Nuclear Pore Complex Studied With Coarse-Grained Modelling and Polarized Light Microscopy
… a crucial role for the phenylalanine-glycine nucleoporins (FG-Nups). I constructed a coarse-grained model to investigate the dynamics of these proteins. My coarse-grained model was able to recapitulate in vivo experimental results previously measured in our lab. My results also suggest that, …
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Tap /Nxf1 N -Terminal Domain Mediates Homotypic Complex Assembly and Is Necessary for Nuclear RNA Export
… with the components necessary for export: nucleoporins, the export cofactor Nxt1, and RNA. Multimeric Tap is capable of forming interactions with the factors necessary for export but does not prefer binding RNA in the multimeric form, suggesting that multimeric Tap is not actively exporting …
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Investigation of Nuclear Pore Complex Protein Interactions and the Implications for Nuclear Transport
… material across the nuclear pore complex and FG-nucleoporins, proteins which compose the complex itself and are of great importance in allowing protected nuclear transport. Molecular dynamics simulations have been performed on transport complexes formed by the transport receptors importin-beta, …
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Investigation of nuclear pore complex protein Interactions and the implications for nuclear transport
… material across the nuclear pore complex and FG-nucleoporins, proteins which compose the complex itself and are of great importance in allowing protected nuclear transport. Molecular dynamics simulations have been performed on transport complexes formed by the transport receptors importin-B, …
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Core Components Of The Nuclear Pore Bind Unique States Of Chromatin And Mediate Polycomb Repression
… traffic, recent studies have shown that Nucleoporins (Nups), the 30 proteins that comprise the NPC, are also involved in chromatin-related processes such as the regulation of genes and chromatin architecture. However, whether individual Nups bind and regulate distinct types of chromatin …
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Development of New Photocrosslinking Approaches to Discover Binding Partners of O-GlcNAc-Modified Proteins
… cell transformation in leukemia. The wild-type nucleoporins are associated with nuclear trafficking. In chapter 2, I demonstrated both NUP98 and NUP98 fusions are O-GlcNAc-modified. Additionally, evidence suggested O-GlcNAc is near the site of interaction based on crosslinking experiments. While …
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Biophysical Characterization of Structure and Dynamics of Nuclear Pore Complex Components
… largest cellular assemblies of proteins, called nucleoporins (nups). The details of NPC architecture, dynamics, and mechanism are still unknown. NPCs can be dissected biochemically into distinct subcomplexes. One of the best-characterized subcomplexes, the Nup84 complex, consists of seven nups …
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Architecture of a Coat for the Nuclear Pore Membrane
… nucleoporin, have been identified, how nucleoporins arrange in NPC, however, is still an enigma. NPC is a highly symmetric protein complex, which contains an eight-fold rotational symmetry across the center of the pore and a two-fold symmetry in the plane of the nuclear envelope. In …
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Structural and biophysical characterization of membrane-coating proteins from the nuclear pore and the primary cilium
… it is a modular assembly formed by -30 different nucleoporins (Nups) arranged into stable subassemblies. One such module is the -600 kDa Y complex, which forms a Y shape and is the best characterized NPC subcomplex with crystal structures accounting for -90% of its total mass. The molecular …
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Proteomic, Bioinformatic and Functional Characterization of the Nuclear Pore Complex of the African Trypanosome
… NPCs are comprised of about 30 proteins, termed nucleoporins (Nups), which are present in multiple copies. The origins and evolution of the nucleus and NPC are not yet clear, although it seems likely that the nucleus arose only once in eukaryotic evolution. To further our understanding of the …
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Analysis of Protein and RNA Interactions of the HIV-1 rev Protein
… and purified six-His tagged versions of these nucleoporins and have demonstrated by<em> in vitro</em> assays that their interaction with Rev in the two-hybrid system must be indirect. Additional two-hybrid experiments have shown that Rev’s NES and the GLFG domains of NUP49 and NUP100 are both …
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Discovery and characterization of prions in Saccharomyces cerevisiae
… a prion. The conformational flexibility of GLFG nucleoporins is critical for the function of the nuclear pore complex, a molecular sieve that regulates all macromolecular transport between the nucleus and cytoplasm.
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The role of human and Drosophila NXF proteins in nuclear mRNA export
… were analyzed for their interaction with RNA, nucleoporins and other known TAP partners in vitro, and tested for their ability to promote nuclear export of a reporter mRNA in vivo. Using these assays, human NXF2, NXF3 and NXF5 were all shown to interact with the known NXF partner p15. NXF2 and …
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Toxic PRn Poly-Dipeptides Encoded by the C9orf72 Repeat Expansion Block Nuclear Import and Export
… comprised of phenylalanine-glycine (FG) domain nucleoporins, and these proteins are essential for the NPC's active transport and passive size-exclusion permeability barrier functions. Purified FG domains of Nup54 and Nup98, both identified in our proteomics study as PRn targets, polymerized into …
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