Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 5 of 5 for “"nucleofection"”.
-
Investigation of the role of the DNA-modifying enzyme, activation-induced cytidine deaminase (AID), in cancer
… reagents was not successful, and therefore, nucleofection was used, which caused successful uptake of the AID-expressing plasmids as well as corresponding controls. However, this method was too harsh for the L1439A cell line as it did not survive post-nucleofection. Based on this result, the …
-
Elucidating the molecular mechanisms of p110δ activation in T cell antigen receptor signalling
… activated T cells via ribonucleoprotein (RNP) nucleofection. This system was used to investigate the roles of the p110δ-associated proteins in TCR-induced PI3K signalling and cytokine production in activated T cells. Collectively, this work has demonstrated the potential of proteomic analysis …
-
Efficient Methods for Ex Vivo Generation of Gene-edited Neutrophils
… show that Cas9 ribonucleoprotein delivery by nucleofection is capable of stable knockout of the highly expressed gene *ITGAM* and that loss of its product CD11b, a key component of the complement receptor CR3, does not impair neutrophil phagocytosis of serum opsonised bacterial bioparticles, …
-
Complementary Strategies to Promote Mesenchymal Stem Cell Differentiation for Ligament Tissue Engineering
… expression of scleraxis was accomplished by nucleofection of C3H10T1/2 cells. Transient mRNA expression, accumulation of the gene product in the cell nucleus, and cell death were observed. Future work will seek to refine the experimental methods, including the development and testing of an …
-
Estrategias de Ingeniería Genética para el mejoramiento de terapias avanzadas basadas en Células Estromales Mesenquimales de gelatina de Wharton de cordón umbilical (CEM-GW) y sus derivados, con potencial de uso clínico
En la última década, los avances en la comprensión del rol de las células estromales mesenquimales (CEM) en los diversos procesos de homeostasis, reparación y regeneración tisular, así como su papel determinante en la regulación de respuestas inflamatorias a nivel local y sistémico, han acelerado …