Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 64 for “"nucleocapsid"”.
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Mutational Analysis of HIV-1 Nucleocapsid Protein and Methods Development for Aptamer Discovery
… mutational and combinatorial analysis of HIV-1 nucleocapsid protein (NCp7). Sixteen mutants of NCp7 were constructed and over-expressed in <em> E. coli </em> . The dissociation constants (K <sub>d </sub> ) between the mutant proteins and the major packaging signal in HIV-1, SL3 RNA, were …
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The Interaction Between Host G3BP and Viral Nucleocapsid Protein Regulates SARS-CoV-2 Replication
… G3BP1/2 are prominent interactors of the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the functional consequences of the G3BP-N interaction in the context of viral infection have remained unclear. Here we used structural and biochemical …
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The Expression of Shuni Virus Nucleocapsid Protein in Nicotiana benthamiana for Use as a Diagnostic Reagent
… of diagnostic reagents centred around the nucleocapsid (N) protein of the SHUV. The N proteins of closely related members of the order Bunyavirales have shown to be highly abundant in infection and cause an immune response in the infected hosts thus making it the ideal target. Using …
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The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus
… and humoral immune responses against the HTNV nucleocapsid protein (NP) elicited by virus infection or DNA vaccination to investigate the immunogenicity of NP. To examine the cellular immune response against HTNV NP, C57BL/6 mice were injected with HTNV intraperitoneal. The NP-specific CD8+ T …
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Functional analysis of the non-coding regions of RNA segments and the nucleocapsid protein of the Uukuniemi virus
… (S, M, and L) encode four structural proteins: a nucleocapsid (N) protein, two glycoproteins (Gn and Gc), and an RNA-dependent RNA polymerase (L protein). In addition, a non-structural protein (NSs) is encoded from the S segment using an ambisense coding strategy. At the termini of the RNA …
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MOLECULAR MECHANISMS OF ATP-MODULATED LIQUID-LIQUID PHASE SEPARATION (LLPS) OF TDP-43 AND SARS-COV-2 NUCLEOCAPSID PROTEIN
An estimated 40% of human proteome has undergone LLPS; delineating its molecular mechanisms may reveal principles undiscovered in physiology and open avenues for pathology. All living cells inexplicably maintain 2-14 mM ATP. Strikingly, in 2017, ATP (> 5 mM) was demonstrated to behave as a …
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Understanding the assembly of simple ssRNA virus nucleocapsids
… from the initial interactions critical for nucleocapsid formation until the morphogenesis which occurs at each maturation stage of the virus’s “life” can entail a wide range of possibilities at every given stage. By whatever seeding event initiates the nucleocapsid formation, the …
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The Structure and Replication of a Parainfluenza Virus
… in the cytoplasm of infected cells. The helical nucleocapsid of the virus appears to form in the cytoplasmic matrix and align under regions of the cell membrane which acquire viral surface projections. Assembly and release of virus particles at the cell surface occur by a budding process …
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The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants
… involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its …
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Modulation of West Nile Virus Capsid Protein and Viral RNA Interaction through Phosphorylation
… protein and also elucidate how the processes of nucleocapsid assembly are regulated. Initial in vitro C protein and viral RNA interaction studies showed that this interaction was not specific. This suggested the presence of a regulatory mechanism to regulate C protein and RNA interaction. There …
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Migration of Nucleocapsids in Vesicular Stomatitis Virus-Infected Cells is Dependent on Microtubules and Actin Filaments
… was used to describe the distribution of the nucleocapsid of VSV during infection, and analyze the importance of the cytoskeleton on this distribution. To determine the mechanism by which nucleocapsids migrate during infection, pulse-chase analysis of protein translation, plaque assays, and …
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Computational studies of biomolecules to explore potential therapeutics for COVID-19 and SAMD9- associated diseases
… exoribonuclease, membrane protein, and nucleocapsid protein. These proteins are functionally significant in the virus life cycle, making them prospective molecular targets for therapeutics. Structure–activity relationships (SARs) analysis of these results revealed that a urea linker in …
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Super-resolved volumetric optical imaging of virus-infected cells
… SARS-CoV-2 infected Vero cells revealed that the nucleocapsid protein of SARS-CoV-2 accumulates at the membrane of the viral replication organelles, strongly suggesting that this is where the encapsidation of the viral genome by the nucleocapsid protein occurs. Imaging the assembly of the viral …
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The Structure and Replication of Semliki Forest Virus, A Mosquito-Borne Animal Virus
… virus particle consists of a nucleoid, or nucleocapsid, 280 A in diameter, closely wrapped in an envelope which consists of a unit membrane 75 A thick coated on its outer surface with projections 110 A long. There appears to be a narrow 10-30 A space between the nucleocapsid and the …
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The analysis of Autographa Californica multiple Nucleopolyhedrovirus EXONO (ORF141) function and its role in virus budding
… of the BV pathway and the mechanism by which nucleocapsids are selected and directed from the nucleus to plasma membrane to form BV. The Autographa californica Multiple Nucleopolyhedrovirus (AcMNPV) gene exon0 (orf141) is known to be required for the efficient production of BV and in this …
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Genetic diversity and taxonomical relationships among the tacaribe serocomplex viruses (family arenaviridae)
… sequences of the glycoprotein precursors and nucleocapsid proteins of 70 arenaviruses, representing the 22 species in the Arenaviridae, indicated that the minimum nonidentity between strains of different species was 15.8% and 11.2%, respectively. Thus, arenaviruses that exhibit at least 15.8% …
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Evaluation of two SARS-CoV-2 immunoassays
… the performance of the Roche Elecsys ® antinucleocapsid (qualitative) and anti-spike (quantitative) SARS-CoV-2 immunoassays to determine whether the performance of the assays is acceptable for diagnostic use in the Groote Schuur Hospital virology/chemistry laboratory, as well as other …
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The Study of Au(III) Compounds and their Interaction with Zinc Finger Proteins
… Cys3His (Cys = cysteine, His = histidine) HIV nucleocapsid zinc finger protein, NCp7. NCp7 is involved in multiple steps of the HIV life cycle, thus making it a desirable drug target. Previous studies from our group show platinated nucleobases such as [Pt(dien)(9-EtG)]2+ (dien = …
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Impatiens Necrotic Spot Virus Resistance in Transgenic Impatiens walleriana and Lycopersicon esculentum
… antisense sequence of open reading frame of INSV nucleocapsid protein (N). The N gene is expected to confer INSV resistance by RNA interference or gene silencing. The presence of transgenes was confirmed by PCR. Transgenic Impatiens was selfed for two generations. Transgenic Micro-Tom was selfed …
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Structural Studies On The Rubella Virus Capsid Protein And Its Organization In The Virion
… low resolution structure of the virus particle. Nucleocapsid assembly studies on the rubella capsid protein has given additional information on the factors affecting formation of the virion cores. Together, these studies enumerate critical differences between rubella virus and alphaviruses that …
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