Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 29 for “"nucleic acid delivery"”.
-
Development of a Structural Nucleic Acid Delivery Vector Technology
The delivery of biomacromolecules such as proteins and nucleic acids to specific cells inside the body remains a critical challenge in contemporary biomedical research. Delivery technologies to accomplish this are ideally safe, effective, versatile, and scalable, but the current commercial …
-
Serum Stable Carbohydrate-Oligoethyleneamine Copolymers for Nucleic Acid Delivery
The delivery of nucleic acids at the tissue and cellular levels remains one of the major hurdles in this scientific area. Since nucleic acids are bulky macromolecules and unstable in the presence of nucleases, vehicles are required to compact them into nanosized particles, offer protection from …
-
Design of Lipid and Polymeric Carriers for Nucleic Acid Delivery
… and develop lipid and polymeric carriers for nucleic acid delivery. These included: i) to develop novel cationic lipids for plasmid, oligonucleotide, and siRNA delivery; ii) to develop a novel polymeric delivery system, polyethylene glycol (PEG) based bio–conjugate, for oligonucleotide …
-
Development of Carbohydrate-based Diblock Polymers for Nucleic Acid Delivery
The delivery of nucleic acids remains the major obstacle for nucleic acid-based therapies such as gene therapy and gene silencing therapies based on RNA interference. In this dissertation we have developed and studied nucleic acid delivery vehicles based on cationic diblock glycopolymers that …
-
Understanding barriers to efficient nucleic acid delivery with bioresponsive block copolymers
The delivery of nucleic acids has the potential to revolutionize medicine by allowing previously untreatable diseases to be clinically addressed. Viral delivery systems have been held back by immunogenicity and toxicity concerns, but synthetic vectors have lagged in transfection efficiency. This …
-
Raft Polymerization Of Ph-Responsive, Diblock Copolymers For Nucleic Acid Delivery Vehicles
… of gene therapy, a variety of non-viral nucleic acid delivery vehicles have been prepared and studied for their transfection efficiencies. Recently, polymeric gene delivery vehicles have gained popularity for their low immunogenicity and high transfection efficiency. With the advent of …
-
Investigating Hybrid Cyclic/Linear and Linear Peptides as Vehicles for Nucleic Acid Delivery
… via creating a mismatch in DNA, respectively. Nucleic acids are anionic and hydrophilic molecule, which cannot enter the cell through passive diffusion and/or interaction with the cell membrane. Cell-penetrating peptides (CPPs) offer a unique carrier for nucleic acid delivery, where positively …
-
Novel Lanthanide Containing Polymers for Nucleic Acid Delivery and Monitoring of Polyplex Dynamics
Nucleic acid therapy holds real promise to offer less severe (lower side effects) as a treatment for life threatening and difficult to treat diseases such as cancer, heart disease or Alzheimer's disease. Theranostic nanomaterials that combine diagnostic imaging and therapeutic delivery, have …
-
Understanding Non-viral Nucleic Acid Delivery Vehicles with Different Charge Centers and Degradation Profiles
… structures of non-viral cationic polymer delivery vehicles, including charge center type, molecular weight and degradability, could significantly affect toxicity, release of nucleic acid and transfection efficiency. Poly(glycoamidoamine)s (PGAAs) contained different carbohydrate and …
-
Efficient Drug and Nucleic Acid Delivery Systems based on Synthetic Amphiphiles with Tuned Oil/Water Interfaces
… times suboptimal, eventually requiring robust delivery systems that can precisely deliver the drugs to the desired tissues. Although many materials have been studied for the generation of drug delivery systems, there is always a need for biomaterials with better properties that can translate …
-
New Sensing Modalities for Bacterial and Environmental Phenomena
… to environmental stimuli via a new nonviral nucleic acid delivery mechanism. This nonviral nucleic acid delivery occurs outside the parameter space of traditional nonviral nucleic acid delivery methods such as electroporation and chemical transformation. The bacteria are immobilized in an …
-
From N to P: Examining Structure-Property Relationships of Ammonium- and Phosphonium-Containing Macromolecules
… of phosphonium macromolecules for nonviral nucleic acid delivery was examined. Phosphonium macromolecules more efficiently complexed nucleic acids than ammonium analogs and butyl-containing phosphonium macromolecules delivered nucleic acids more effectively than the ammonium analog. …
-
Novel Lipid Polymer Design for Nucleic Acids Delivery
… also depends on the development of reliable delivery systems for single-guide RNA (sgRNA)-Cas9 complexes. Lipid nanoparticles (LNPs) have emerged as one of the most successful non-viral carriers for nucleic acids, highlighted by the approval of the first small interfering RNA (siRNA) therapy …
-
Engineering Lipid Nanoparticle-Mediated Delivery of Nucleic Acids to Lymphoid Organs and Immune Cells
Ribonucleic acids (RNA) and deoxyribonucleic acids (DNA) hold promise for continued application in the treatment of genetic diseases. Short interfering RNA (siRNA) and microRNA (miRNAs) have been used to silence disease-causing genes. Longer messenger RNA (mRNA) and plasmid DNA (pDNA) have been …
-
Development of polymer - lipid nanoparticles for potent mRNA delivery to the lung
… to be successfully translated. The field of nucleic acid delivery, therefore, is largely concerned with the development of materials which can encapsulate mRNA and facilitate its transport into cellular cytoplasm in vivo. Many lipid nanoparticles originally designed to deliver short …
-
Poly(glycoamidoamine)s: Understanding their Structure and Structure-Bioactivity Relationships
… of biomaterials that are used in the therapeutic delivery system. This dissertation is dedicated towards understanding the hydrolysis pattern of plasmid DNA (pDNA) delivery vehicles comprised of poly(glycoamidoamine)s (PGAAs) under physiological conditions and effects of subtle changes in the …
-
Non-covalent Intermolecular Interactions in Polymer Design: Segmented Copolymers to Non-viral Gene Delivery Vectors
… of functionalized polymers with non-viral nucleic acids delivery vehicles. The ability to control the intermolecular interactions of a given system allow for tailoring of that system to yield a desired outcome, whether it is a copolymers mechanical properties or the colloidal stability of a …
-
Cationic Polymers and Polymeric Micelles as Plasmid DNA and CRISPR-Cas9 Ribonucleoprotein Delivery Vehicles
… an alternative, holds great potential by direct delivery of genetic materials such as nucleic acids and genome editing machineries into human body to achieve long-term therapeutic protein expression, malfunctioning gene silencing, and native gene alternation. As a crucial step towards gene …
Page 1 of 2