Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"nucleic acid amplification"”.
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Silaffin Tagged Polymerase for Nucleic Acid Amplification
… sensitive and specific loop mediated isothermal amplification (LAMP) assays. Fusing the enzyme with a silaffin tag (R5) and a fluorescent protein (mCherry) enabled rapid and cost-effective purification through unmodified silica immobilisation and built-in visual tracking from protein production …
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Development of Bioassays Based on Nucleic Acid Amplification and Next-Generation Sequencing
… with poly(ethylene glycol) to link DNA amplification to target protein concentration. Electrostatic neutralization and steric cloaking of the PCR-amplifiable DNA labels by avidin and PEG coating reduces non-specific “stickiness” and enhances assay sensitivity. We further optimized the …
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Novel and newer nucleic acid amplification tests for the diagnosis of TB
… of the promising novel loop mediated isothermal amplification (LAMP) assay on sputum, and that of the semi-automated Xpert MTB/RIF (Xpert) test on non-sputum specimens (bronchoalveolar lavage fluid [BALF], tracheal aspirates, and cerebrospinal fluid [CSF]) from South African patients with …
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Development and clinical performance of nucleic acid amplification techniques for the diagnosis of Strongyloides stercoralis
… the decision was made to evaluate and validate nucleic acid amplification techniques (NAATs) for the diagnosis of S. stercoralis for use in a well- resourced specialist referral parasitology laboratory. A novel loop mediated isothermal amplification (LAMP) assay was also developed for use in …
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Diagnostic Accuracy of Nucleic Acid Amplification Tests in Urine for Pulmonary Tuberculosis: A Meta-Analysis
… the diagnostic accuracy of tuberculosis nucleic acid amplification tests (NAATs) in urine samples for active pulmonary tuberculosis (PTB). <bold>Design:</bold> Systematic review and meta-analysis. Electronic databases and reference lists were searched without language, setting or time …
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On-chip parallel detection of foodborne pathogens using loop-mediated isothermal amplification
… A point-of-care device capable of performing nucleic acid amplification will enable effective detection of infectious agents in multiple settings, allowing better enforcement of food safety regulations. Current protocols and practices for control of agents responsible for foodborne disease …
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Transistor-based biosensing: expanding the functionality of field effect transistors
… of Polymerase Chain Reaction (PCR)-based amplification of nucleic acids by Kary Mullis in 1983(1), researchers have spent significant efforts to improve the sensitivity and selectivity of PCR assay and have dramatically enhanced its application(2). PCR is now an integral tool of modern …
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The intersection of the HIV epidemic and blood donation in South Africa
… which may escape detection by HIV antibody and nucleic acid amplification testing (NAT). My PhD research used epidemiologic, incidence modelling and mixed-method qualitative research techniques to assess the impact of undisclosed ART use among blood donors on the safety of the country's blood …
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Human papillomavirus Genotype Distribution and Co-infection with Sexually Transmitted Pathogens in Reproductive age Women in Urban Gambia
… risk characteristics associated with HPV. Nucleic acid amplification techniques and DNA sequencing were carried out to determine the HPV genotype using PGMY09/11 consensus primers. Phylogenetic analysis was carried out on the Gambian HPV sequences to further confirm the identification of …
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Novel Diagnostic approach for tuberculosis diagnosis
… to compare it to other commercially available nucleic acid amplification assays and to standard microbiological smear and culture. The GeneXpert system performs real time, nested PCR from sputum and provides a result within two hours of sampling. The result includes a semi-quantitative …
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Designing Next Generation Nucleic Acid Diagnostics Using Synthetic Biology and Artificial Intelligence
Nucleic Acid Testing (NAT) is an indispensable tool for effective disease diagnosis. Analyzing pathogen and host RNA or DNA often provides otherwise unobtainable information necessary for proper patient treatment. Unfortunately, a number of technical barriers prevent the expansion of NAT technology …
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Increased sensitivity for lateral flow immunoassays through signal amplification methods
… and precipitation of visible dyes, and (3) nucleic acid amplification test (NAAT)-based signal enhancement. Using the enzymatic amplification-based detection, we developed a prototype of a point-of-care (POC) device including a dry conjugate pad and the assay sensitivity was compared with a …
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Epidemiological profile of Tuberculosis in Sinazongwe District in the context of HIV/AIDS
… should consider employing the rapid automated Nucleic Acid Amplification Test to improve diagnosis on TB among HIV patients and strength the Direct Observed Therapy through health education and sensitization
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Epidemiological profile of Tuberculosis in Sinazongwe District in the context of HIV/AIDS
… should consider employing the rapid automated Nucleic Acid Amplification Test to improve diagnosis on TB among HIV patients and strength the Direct Observed Therapy through health education and sensitization
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Configurable and Up-Scalable Microfluidic Life Science Platform for Cell Based Assays by Gravity Driven Sequential Perfusion and Diffusion
… microfluidic tmRNA purification and real time nucleic acid sequence based amplification (NASBA) device. The device is manufactured using soft lithography and a unique silica bead immobilization method for the nucleic acid micro purification column. The integrated device produced a …
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The Exploration and Development of Focused Ultrasound Extraction (FUSE) for the Rapid Release of DNA from Complex Tissue Matrices
… of simpler, faster, and more accessible nucleic acid amplification tests (NAATs) that have enabled point-of-contact (POC) DNA testing, delivering real-time results in resource-limited settings. Despite these advancements, the DNA sample preparation process is laborious, …
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Virtual microfluidics : a novel single-cell technology based on diffusion-restricted reaction that makes high-quality low-input genomic research accessible
… robust platform for the compartmentalization of nucleic acid amplification reactions. We applied whole genome amplification (WGA) to purified DNA molecules, cultured bacterial cells, human gut microbiome samples, and human cell lines in the virtual microfluidics system. We demonstrated …
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The application of loop mediated isothermal amplification for the detection of the sexually transmitted pathogens Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis, at the point of care.
… fully integrated POC system, combining automated nucleic acid extraction in a centrifugally operated microfluidic disk (the LabDisk), with loop mediated isothermal amplification (LAMP) and optical detection, capable of detecting the sexually transmitted pathogens Neisseria gonorrhoeae, Chlamydia …
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Immunological and Molecular Techniques for Point-of-Care Diagnostics
… with their secreted proteins and constituent nucleic acids are the principal analytical targets. Rapid screening tests, suitable for use as point-of-care (POC) devices by minimally trained personnel in decentralized laboratories and in the field, can be useful in diagnosing patients presenting …
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