Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"nuclear transport"”.
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Investigating nuclear transport proteins as secreted cancer biomarkers
… expression analysis identified members of the nuclear transport protein family as significantly upregulated in cervical cancer biopsies compared to normal cervical epithelial tissues. These results were validated at both mRNA and protein levels, and similar upregulation observed in oesophageal …
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Investigating the role of nuclear transport proteins secreted by cancer cells
… studies in our laboratory have shown that the nuclear transport protein Importin 1 (Kpnb1) is upregulated in several types of cancer and is crucial to cancer cell survival. Recent findings from our laboratory showed that Kpnb1, amongst other proteins is secreted into the extracellular space by …
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Analysis of nuclear transport signals of the human apurinic/apurimidinic endonuclease (APE1/REF1)
The nuclear localization signal (NLS) in human apurinic/apyrimidinic endonuclease (APE1), a key protein in both DNA base excision repair and transcriptional regulation, has not been analyzed in detail. We examined the role of specific residues in nuclear translocation of APE1, using green …
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Investigation of Nuclear Pore Complex Protein Interactions and the Implications for Nuclear Transport
… exchanging proteins and RNA, for a variety of nuclear and cytoplasmic processes which act in concert. The nuclear pore complex is responsible for controlling the transport of large molecules into and out of the cell nucleus. It is perhaps the largest protein structure in eukaryotic cells, and …
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Investigation of nuclear pore complex protein Interactions and the implications for nuclear transport
… exchanging proteins and RNA, for a variety of nuclear and cytoplasmic processes which act in concert. The nuclear pore complex is responsible for controlling the transport of large molecules into and out of the cell nucleus. It is perhaps the largest protein structure in eukaryotic cells, and …
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Shield and Shuttle: Importins as Protective Chaperones and the Nuclear Transport Mechanism of H2A-H2B
The active transport of macromolecules from the cytoplasm to the nucleus is carried out by a sub-class of Karyopherins called Importins. It was hypothesized back in the early 2000s that importins could have dual functions as 1) nuclear importers and 2) molecular chaperones. This thought stems back …
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Expression and regulation of the nuclear transport proteins, Crm1 and Kpnß1, in cervical cancer and transformed cells
Includes abstract. Includes bibliographical references (leaves 204-223).
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Investigating the effect of conventional chemotherapies, Cisplatin and Doxorubicin, in combination with a nuclear transport inhibitor, INI-43, on cerfical cancer cells
… targets and found that Kpnβ1, a member of the nuclear transport protein family is overexpressed and necessary for the survival of cervical cancer cells. Inhibition of Kpnβ1 with a novel Inhibitor of Nuclear Import; INI-43 inhibits Kpnβ1- associated nuclear import pathways and results in cancer …
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Characterisation of mutants involved in Phytochrome A nuclear import and signal transduction
… to isolate and characterise Phytochrome A (phyA) nuclear transport mutants. The screening was performed in two steps. First, the mutagenised seedlings were selected on the basis of differences in hypocotyl length. In a second step, the selected putants were screened for altered localisation of …
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Differenzielle Effekte von Presenilin-Mutationen auf die Generierung des Amyloid-ß-Peptids (Aß) und die Endoproteolyse des Notch-Rezeptors
… was increased, endoproteolysis of Notch and nuclear transport of its cytoplasmic domain was significantly inhibited. These results demonstrate differential effects of PS proteins in the endoproteolysis of the ß-amyloid precursor protein and Notch.
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Design of an in vitro assay to optimize assembly of nanoparticle-tagged nuclear import complexes
… importin-a and importin-3 mediate classical nuclear transport, an essential and highly conserved example of protein complex formation in eukaryotic cells. Together, these two proteins form a chaperone complex that recognizes a nuclear localization signal (NLS), which is a short peptide …
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Communication between layers in biological transcriptional networks
… (HS), histone modifiers (HM) and remodelers, nuclear processing (NP), and nuclear transport (NT) proteins. In order to gain a holistic understanding of the non-linear process of transcription, our work examines the communication between all five forementioned classes (or layers) of regulators. …
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Inter-Organellar Nucleic Acid Communication by a Mitochondrial tRNA Regulates Nuclear Transcription
… of human cells. Focused studies of one such nuclear-transported mito-tRNA-asparagine (mito-tRNA-Asn) revealed that its cognate charging enzyme (NARS2) is also present in the nucleus. Nuclear localization of mito-tRNA-Asn and NARS2 was dependent on the VDAC1 mitochondrial channel and …
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Identification of Oncogenic KRAS-Associated Vulnerabilities in Non-Small Cell Lung Cancer
… analysis. This identified components of nuclear transport machinery as selectively essential for KRAS mutant NSCLC lines. We found that pharmacological inhibition of a key nuclear export receptor, XPO1 (a.k.a. CRM1), was sufficient to induce robust and selective apoptosis in KRAS mutant …
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Kap-[beta]2 Recognition of Cargoes Involved in Neurodegenerative/Neurodevelopmental Diseases
Karyopherin β2 is a nuclear-import receptor that transports many RNA-binding proteins into the nucleus by recognizing within the cargoes a unique nuclear-localization signal known as the PY-NLS. Several of Kapβ2 cargoes are mutated in neurological diseases. Two such proteins of interest to my …
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Copper-64 radiopharmaceuticals for receptor-mediated tumor imaging and radiotherapy
… to 111In-DOTA-sst2-ANT in the same model. Nuclear localization of copper-64 from two somatostatin analogues differing in their chelate stability strengthened the hypothesis of copper-64 dissociation from the bifunctional chelator prior to trafficking to the nucleus. However, the increased …
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Plasticity of the Nuclear Pore Complex Revealed With Proteomics
… and have successfully applied it to the yeast nuclear pore complex (NPC), one of the largest and most elaborate protein machines in the cell. NPC is a massive protein assembly situated in the nuclear envelope and mediating macromolecular transport between the nucleus and cytoplasm. To study the …
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Explorer l'impact de l'élimination de la charge nette cationique des signaux de localisation nucléaire (NLS) sur le ciblage par l'importine-α
… weight molecules to freely diffuse through nuclear pores, making it challenging to target. Nuclear transport is a vital component of normal functioning in eukaryotic cells. Specialized transport proteins called importins recognize nuclear localization signals (NLSs) on proteins in the …
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Deciphering the molecular basis of the global emergence of non-tuberculous Mycobacteria as important human respiratory pathogens
… led to the identification of importazole, a nuclear transport inhibitor, as a compound that inhibits intracellular replication. Further mechanistic studies showed that importazole has direct antibacterial activity against M. avium, causing a reversible increase in bacterial cell length and …
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