Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"nuclear transfer"”.
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Somatic cell interspecies nuclear transfer
The low efficiency of the nuclear transfer (NT) procedure requires large number of oocytes to produce embryos and live offspring. A series of experiments were conducted to evaluate the ability of the bovine cytoplast to reprogram nuclei from horses and llamas. In a preliminary study, equine oocytes …
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Somatic cell nuclear transfer in zebrafish
Thesis (Ph. D.)--Michigan State University. Comparative Medicine and Integrative Biology, 2010
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Nuclear Transfer With Porcine Embryonic Stem Cells
… most efficient treatment for production of pronuclear stage cytoplasm. Results from NT experiments indicated that neither enucleated, unactivated oocytes nor enucleated, activated oocytes promoted development of NT embryos constructed from porcine ES cells beyond the four-cell stage. However, …
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Cloning, stem cells and epigenetic reprogramming after nuclear transfer
… more specified fates and specialized functions. Nuclear transfer (NT) experiments have demonstrated the epigenetic nature of development and showed, that although differentiated cells have a very limited developmental potential, the nuclei of these cells retain the potency to direct embryogenesis …
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Gene expression and imprinting in mice cloned by nuclear transfer
Most cloned mammals derived by nuclear transfer (NT) die during gestation, and those surviving to birth frequently show increased birth weights, enlarged dysfunctional placentas, and neonatal mortality associated with respiratory distress and metabolic abnormalities. These abnormalities may reflect …
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Gene injection in the bovine: effect of time of microinjection and nuclear transfer technologies
… obtained when injection is performed early in pronuclear formation (7% (11/161) at 11; 4% (61159) at 15; 1 % (1/165) at 19 haf; p<0.05). Experiment 2 examined the effect of microinjection of DNA into the germinal vesicle (gv) of bovine oocytes on subsequent development and detection of the …
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Effects of somatic cell nuclear transfer on transcripts related to IGF signaling, EGF signaling and cell adhesion during the peri-implantation period in cattle
Up to 70% of somatic cell nuclear transfer (SCNT)–derived bovine pregnancies are lost during the peri-implantation period of gestation. Given the physiology of blastocyst implantation and clinico-pathological features that characterize SCNT pregnancies, we hypothesized that insulin-like growth …
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DNA methylation in the early porcine embryo
… parthenogenetic activation, and somatic cell nuclear transfer are powerful procedures in the production of animals for agriculture, basic research, and biomedical research. Research using these techniques has produced important insights into the basic mechanisms of gametogenesis, …
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Characterization of porcine adipose tissue-derived stem cells
Nuclear transfer (NT) results in low success rates and research has reported problems with the welfare of cloned animals produced. Evidence suggests the use of a less differentiated donor cell, such as a stem cell, may lead to an increase in the efficiency of the NT procedure. Stem cell …
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Mitochondrial DNA replication in pre-implantation embryonic development
… the oocyte, its replication being regulated by nuclear-encoded replication factors. It was hypothesised that mtDNA replication is highly regulated in oocytes, pre-implantation embryos and embryonic stem cells (ESCs) and that this may be disrupted following nuclear transfer (NT). MtDNA copy …
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Involvement of mitochondrial transcription factor A (TFAM) in porcine gametogenesis and preimplantation embryo development
… and zygotes reconstructed by somatic cell nuclear transfer (SCNT). Boar sperm TFAM was found to be posttranslationally modified by ubiquitination and relegated to sperm tail principal piece in fully differentiated spermatozoa. These data suggest that mitochondrial transcription factor TFAM …
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DNA methylation in early mammalian development
… artificial reprogramming process, somatic cell nuclear transfer, in mouse, and find that most dynamics are conserved but occur at a smaller magnitude after artificial reprogramming. I conclude this thesis with a summary of the chapters and a brief discussion of ongoing and future work.
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The Rhetoric of Repugnance: Popular Culture and Unpopular Notions in the Human Cloning Debate
… announcement of Dolly-the-sheep's birth through nuclear transfer. Using methodologies drawn from the social shaping of technology (SST) and rhetoric of science, my analysis demonstrates how individuals and institutions, including the media, ethicists, policymakers, and legislators, appropriated …
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Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
Cloning by somatic cell nuclear transfer (SCNT) forces cells to lose their lineage-specific epigenetic marks and become totipotent again. This reprogramming process often results in epigenetic and transcriptional aberrations that compromise development. Development rates after SCNT can thus serve …
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Developing embryo technologies for the eland antelope (Taurotragus oryx)
… intracytoplasmic sperm injection and nuclear transfer resulted in embryonic development. In conclusion, the bovine embryo culture study suggests that the beneficial effects of amino acids are influenced by the base medium and glucose plays more important roles in non-ATP producing …
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The role of transcription factors in somatic cell nuclear reprogramming by eggs and oocytes
Somatic cell nuclear reprogramming (SCNR) by eggs is a way to forcibly transform the nuclei of terminally differentiated somatic cells to an embryonic state and gain totipotency (Gurdon et al., 1958). Additionally, induced pluripotency is applied to transform identities of somatic cells to induced …
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Induced pluripotency and reprogramming by defined factors
… and cellular differentiation. Somatic cell nuclear transfer experiments (SCNT) provided unequivocal proof of nuclear equivalency between adult somatic cells. However the molecular mechanisms of somatic cell reprogramming have remained largely a mystery. Recent advancements in epigenetic …
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The chick embryo amnion as an in vitro culture system for IVF and NT embryos
… controls. In Experiment II, it was shown that nuclear transfer bovine embryos cultured in the chick amnion reached the blastocyst stage at rates equal to controls and were capable of producing pregnancies following transplantation into recipient females. In a subsequent experiment, a method of …
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