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Showing 1 to 20 of 31 for “"nuclear pore complex (NPC)"”.
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Structural and biochemical characterization of nuclear pore complex structural scaffold sub-complexes
The nuclear pore complex (NPC) is a large, modular protein assembly that regulates nucleocytoplasmic transport in all eukaryotes. The ~60-120 MDa NPC is a modular assembly of multiple copies of ~30 distinct proteins that are arranged into biochemically distinct sub-complexes. We believe that the …
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Mechanism of Nuclear Protein Import and Export an In Vitro Approach
… of the signal, delivery of the protein to the nuclear pore complex (NPC) and its subsequent translocation across the NPC. We have biochemically analyzed the mechanism of nuclear protein import and export using all recombinant factors from Saccharomyces cerevisiae. Using solution binding assays, …
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The Intracellular Changes and the Localization of Core particles by Hepatitis B Virus P Protein-Deficient Mutant
… using anti-HBc antibody. Microtubules, vimentin, nuclear pore complex (NPC) were visualized using mouse anti-tubulin, mouse anti-vimentin, and mouse anti-NPC antibody, respectively. RESULTS: P-deficient mutant core particles co-localized with microtubule, NPC, and vimentin, like HBV wild type (wt) …
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Nucleocytoplasmic Localization of MAPKs
… may translocate to the nucleus to phosphorylate nuclear targets. Previous findings from our laboratory showed that a constitutively active and nuclear form of the MAPK ERK2 is sufficient for transformation of immortalized fibroblasts (Robinson MJ et al,1998). However the mechanisms of nuclear …
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Biochemical Analysis of the Protein-Protein Interactions Involved in Karyopherin-Mediated Transport Across the Nuclear Pore Complex
<p>Nucleocytoplasmic transport occurs through the nuclear pore complex (NPC), which in yeast is a highly symmetric ~50 MDa complex consisting of approximately 30 different proteins. Small molecules can freely exchange through the NPC, but macromolecules larger than ~40 kDa such as proteins, mRNAs, …
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Characterisation of ALADIN’s function during cell division
… of chromosome segregation. To achieve such complex and multiple functions, cells have evolved mechanisms by which one protein can participate in numerous events on the cell life. Over the past few years, an increasing number of functions have been assigned to the proteins of the nuclear pore …
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Synthetic ground truth of biological shapes — Simulating variable Nuclear Pore Complexes for Microscopy
The Nuclear Pore Complex (NPC) is the only passageway for macromolecules between the nucleus and cytoplasm and an important reference standard in microscopy: it is intrinsic to cells, has a high copy number, it is massive and stereotypically arranged. The average architecture of NPC proteins has …
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Biophysical Characterization of Structure and Dynamics of Nuclear Pore Complex Components
<p>The Nuclear Pore Complex (NPC) mediates nucleo-cytoplasmic transport in all eukaryotes and is among the largest cellular assemblies of proteins, called nucleoporins (nups). The details of NPC architecture, dynamics, and mechanism are still unknown. NPCs can be dissected biochemically into …
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Molecular mechanisms of gating and selectivity in transport channels
Transport channels and pores are of fundamental importance for translocation of molecules across the otherwise-impermeable biological membrane. As a conduit for the passage of material, specific membrane channels must accomplish the task of selective transport of molecules, while preventing …
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Structural studies of large architectural nucleoporins and coat proteins
The Nuclear Pore Complex (NPC) is a ~50 MDa protein complex that forms the sole conduit for macromolecular transport across the nuclear envelope. It assembles from ~30 proteins, termed nucleoporins or nups, symmetrically arranged about a central 8-fold axis. Some nucleoporins also contribute to …
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Plasticity of the Nuclear Pore Complex Revealed With Proteomics
… and have successfully applied it to the yeast nuclear pore complex (NPC), one of the largest and most elaborate protein machines in the cell. NPC is a massive protein assembly situated in the nuclear envelope and mediating macromolecular transport between the nucleus and cytoplasm. To study the …
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Mechanisms of piRNA biogenesis and co-transcriptional silencing of transposable elements in Drosophila
… export is closely linked to the assembly of peri-nuclear condensates of the helicase fs(1)Yb (Yb). Furthermore, some subunits of the Nuclear Pore Complex (NPC) are also required for the production flam-derived piRNAs, thus suggesting the evolution of a specialised machinery that couples nuclear …
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Structural and biochemical characterization of LINC complexes
The nuclear envelope (NE) is comprised of a double membrane bilayer that physically separates the nucleoplasm from the cytoplasm. Information can be transmitted through the NE by molecular exchange through the nuclear pore complex (NPC) and by transduction of mechanical forces mediated by the …
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A link between c. Elegans morphogenesis and mrna export
… a thermo-sensitive t2160 mutant allele of nuclear export 1, nxf-1. nxf-1/TAP encodes a protein that is required for mRNA export from the nucleus to the cytoplasm. To better understand the role of NXF-1 in C. elegans embryonic morphogenesis, we analysed the expression of several apical …
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Conformational Dynamics of the Nuclear Pore Complex Studied With Coarse-Grained Modelling and Polarized Light Microscopy
… I explored the organization and dynamics of the nuclear pore complex (NPC) through coarse-grained modelling and polarized-total internal reflection fluorescence (pol-TIRF) microscopy. The NPC is a large (~120 MDa in humans) macromolecular assembly that controls the flow of molecules into and out …
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Biophysical and structural characterization of components from the nuclear pore complex and the ubiquitin pathway
… of biological evolution. One such system is the nuclear envelope (NE), composed of an inner and outer membrane, used to form a nucleus and enclose the cell's genome. Access to the nucleus from the cytoplasm is mediated by a massive macromolecular machine called the nuclear pore complex (NPC). The …
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Analyzing the role of a cellular mechanism in HIV infection and Evaluation of novel antiviral compounds
… is the active transport of the pre-integration complex (PIC) into the nucleus. The mechanism remains elusive, yet in the case of HIV the viral protein R (Vpr), a small 14kDa accessory protein, is one candidate possibly implicated in the process. The protein stays associated to the PIC until …
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Proteomic, Bioinformatic and Functional Characterization of the Nuclear Pore Complex of the African Trypanosome
… the boundary of a double membrane, known as the nuclear envelope (NE). Transport across the NE is mediated by large protein assemblages known as nuclear pore complexes (NPCs). Yeast and vertebrate NPCs are comprised of about 30 proteins, termed nucleoporins (Nups), which are present in multiple …
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Toxic PRn Poly-Dipeptides Encoded by the C9orf72 Repeat Expansion Block Nuclear Import and Export
… proteomics approach, demonstrating that the nuclear pore complex (NPC) as a major binding target. The biological significance of the PRn peptide-NPC interaction became apparent when we observed major defects in both mature mRNA export to the cytoplasm and protein import into the nucleus after …
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