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Showing 1 to 20 of 65 for “"nuclear pore complex"”.
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The Nuclear Pore Complex: Novel Glycoprotein Constituents
<p>Nuclear pore complexes are large, uniform organelles with a mass estimated to be on the order of 10<sup>8</sup> daltons. They are ubiquitous components of the nuclear envelope, providing a channel through which macromolecular transport between the nucleus and cytoplasm occurs. Thus far, almost …
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Plasticity of the Nuclear Pore Complex Revealed With Proteomics
… and have successfully applied it to the yeast nuclear pore complex (NPC), one of the largest and most elaborate protein machines in the cell. NPC is a massive protein assembly situated in the nuclear envelope and mediating macromolecular transport between the nucleus and cytoplasm. To study the …
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Discrete 3D Model of Molecular Diffusion Through the Nuclear Pore Complex
Nuclear pore complexes (NPCs) are passageways that exist within the nuclear envelope (NE) of a eukaryotic cell. Molecular cargo travel through the passageways to either import to the nucleus or export to the cytoplasm of the cell. Efficient export of certain cargo is necessary for maintained health …
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Biophysical Characterization of Structure and Dynamics of Nuclear Pore Complex Components
<p>The Nuclear Pore Complex (NPC) mediates nucleo-cytoplasmic transport in all eukaryotes and is among the largest cellular assemblies of proteins, called nucleoporins (nups). The details of NPC architecture, dynamics, and mechanism are still unknown. NPCs can be dissected biochemically into …
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Structural and biochemical characterization of nuclear pore complex structural scaffold sub-complexes
The nuclear pore complex (NPC) is a large, modular protein assembly that regulates nucleocytoplasmic transport in all eukaryotes. The ~60-120 MDa NPC is a modular assembly of multiple copies of ~30 distinct proteins that are arranged into biochemically distinct sub-complexes. We believe that the …
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Investigation of Nuclear Pore Complex Protein Interactions and the Implications for Nuclear Transport
… exchanging proteins and RNA, for a variety of nuclear and cytoplasmic processes which act in concert. The nuclear pore complex is responsible for controlling the transport of large molecules into and out of the cell nucleus. It is perhaps the largest protein structure in eukaryotic cells, and …
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Investigation of nuclear pore complex protein Interactions and the implications for nuclear transport
… exchanging proteins and RNA, for a variety of nuclear and cytoplasmic processes which act in concert. The nuclear pore complex is responsible for controlling the transport of large molecules into and out of the cell nucleus. It is perhaps the largest protein structure in eukaryotic cells, and …
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Proteomic, Bioinformatic and Functional Characterization of the Nuclear Pore Complex of the African Trypanosome
… the boundary of a double membrane, known as the nuclear envelope (NE). Transport across the NE is mediated by large protein assemblages known as nuclear pore complexes (NPCs). Yeast and vertebrate NPCs are comprised of about 30 proteins, termed nucleoporins (Nups), which are present in multiple …
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Structure and Dynamic Studies of the Nuclear Pore Complex at the Single-Molecule Level
Nuclear pore complexes (NPCs) are large macromolecular structures forming the only known direct route across the double bilayer membrane of the nuclear envelope. The NPC structure has been extensively explored in an effort to elucidate the mechanisms by which they control transport. Many of these …
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Structural elucidation of a common architecture of the nuclear pore complex and COPIl vesicle coats
Nuclear pore complexes (NPCs) are massive protein assemblies that perforate the nuclear envelope and form the exclusive passageway for nucleocytoplasmic transport. NPCs play critical roles in molecular transport and a myriad of other cellular processes. Elucidation of the structure of the NPC is …
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Biophysical and structural characterization of components from the nuclear pore complex and the ubiquitin pathway
… of biological evolution. One such system is the nuclear envelope (NE), composed of an inner and outer membrane, used to form a nucleus and enclose the cell's genome. Access to the nucleus from the cytoplasm is mediated by a massive macromolecular machine called the nuclear pore complex (NPC). The …
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Conformational Dynamics of the Nuclear Pore Complex Studied With Coarse-Grained Modelling and Polarized Light Microscopy
… I explored the organization and dynamics of the nuclear pore complex (NPC) through coarse-grained modelling and polarized-total internal reflection fluorescence (pol-TIRF) microscopy. The NPC is a large (~120 MDa in humans) macromolecular assembly that controls the flow of molecules into and out …
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Studies on the interaction of HSV-1 multifunctional protein ICP27 with the nuclear pore complex proteins
… levels. ICP27 contains both a nuclear localization signal and a nuclear export signal and shuttles between the nucleus and the cytoplasm. It interacts directly with various cellular proteins including REF and cellular mRNA export receptor TAP to export viral mRNAs. However other …
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Biochemical Analysis of the Protein-Protein Interactions Involved in Karyopherin-Mediated Transport Across the Nuclear Pore Complex
<p>Nucleocytoplasmic transport occurs through the nuclear pore complex (NPC), which in yeast is a highly symmetric ~50 MDa complex consisting of approximately 30 different proteins. Small molecules can freely exchange through the NPC, but macromolecules larger than ~40 kDa such as proteins, mRNAs, …
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Selective transport of the nuclear pore complex studied through all-atom and coarse-grained molecular dynamics simulations
… connecting the cell’s nucleus and cytoplasm, nuclear pore complexes (NPCs) enable and control the import and export between cell’s cytoplasm and nucleoplasm. The NPC allows small molecules (<40 KDa) to diffuse freely into and out of the nucleus, while excluding large molecules, which are only …
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Molecular characterization of components of the nuclear pore complex and the nuclear import system in Saccharomyces cerevisiae
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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A nanobody suite for yeast scaffold nucleoporins provides details of the Y complex structure and nuclear pore complex assembly
Nuclear pore complexes (NPCs) are the main conduits for molecular exchange across the nuclear envelope. The NPC is a modular assembly of ~500 individual proteins, called nucleoporins or nups, that can be classified into three categories: 1. Stably associated scaffolding nups, 2. Peripheral nups, …
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Identification and Characterization of Mlp1p and Mlp2p : Molecular Components of Filaments Localized at the Interface between the Nuclear Pore Complex and Nuclear Interior
… material between the nucleus and the cytoplasm. Nuclear pore complexes (NPCs) provide the only known sites for exchange of material across the nuclear envelope (NE). A wealth of evidence has accumulated throughout the years that strongly suggest that the NPCs are structurally linked to the …
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Examination of Brain-Fatty Acid Binding Protein Interaction with Hydrophobic Ligands and Micelles
… and protein-protein interactions. FABP7 bears a nuclear localization signal that allows ligand-dependent transport to the nucleus through the nuclear pore complex. Once in the nuclear pore complex, FABP7 can then exchange its cargo with transcription factors. FABP7 can also exchange cargo and …
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