Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 16 of 16 for “"nuclear periphery"”.
-
Analyzing the effects of Cis-elements and trans-factors on the stability of the Gal1 mRNP
… conditionally expressed genes relocate to the nuclear periphery upon activation. Moreover, these genes can be retained at the nuclear periphery for a considerable time after transcriptional shutoff. Sequence specific DNA binding proteins, transcription, chromatin remodeling, and mRNP quality …
-
Mechanistic and Epigenetic Partitioning of Lamina-Associated Chromatin Revealed by a Genome-Wide Imaging Screen
The nuclear periphery has long been recognized as a site of heterochromatin organization, where lamina-associated domains (LADs) play a crucial role in transcriptional repression and genome stability. However, the molecular mechanisms that tether LADs in human cells remain poorly defined. To …
-
BAC transgene arrays as a model system for studying large-scale chromatin structure
… including transcription level and intranuclear positioning. Using this system, we showed that tandem arrays of housekeeping gene loci form open large-scale chromatin structure independent of their genomic integration sites, including insertions within centromeric heterochromatin. This …
-
Characterisation of the Arabidopsis mid-SUN proteins at the plant NE
… and Cytoskeleton (LINC) complex formation at the nuclear envelope (NE), similarly to Cter-SUN domain proteins. In addition to interacting with LINC complex components, they have been reported to localise to both the NE and endoplasmic reticulum (ER). There is also evidence that they interact with …
-
Novel plant nuclear envelope-associated coiled-coil proteins
The nuclear envelope (NE) is a double lipid bilayer enclosing the eukaryotic genome. The metazoan nucleoskeleton includes the peripheral lamina and the internal nucleoskeleton. The lamina is composed of a network of intermediate filament (IF) proteins called lamins, as well as lamin- and/or …
-
Topology of Genes in Mammalian Cell Nuclei with Special Emphasis on the MLL Gene and Its Translocation Partners
… interphase nucleus of hematopoietic cells. The nuclear localization of a gene relative to the nuclear center and the nuclear periphery was determined. To provide a general understanding of the distribution pattern of a given locus within the nucleus, the nuclear volume was divided into five …
-
A Nuclear Mechanoresponse to Morphogenesis Drives Acquisition of Neuroectoderm Lineage Competence During Pluripotent Cell Differentiation
… forces to the nucleus and influence cell state. Nuclear mechanosensitivity can result in changes a cell’s molecular-epigenetic state and regulate gene expression during differentiation, thereby influencing cell fate. During implantation, pluripotent cells within the embryo undergo an apical …
-
Myosin-Like Proteins in S. cerevisiae: Multifunctional, Structural Components of the Nuclear Envelope
<p>The nuclear envelope (NE) separates the genetic material from the rest of the cell, delimits and defines the nucleus, organizes the intranuclear architecture and serves as a regulator for multiple nuclear processes. In all eukaryotes, filamentous coiled-coil proteins are associated with the …
-
Epigenetic Regulation of Skin Development and Postnatal Homeostasis The role of chromatin architectural protein Ctcf in the control of Keratinocyte Differentiation and Epidermal Barrier Formation
… loci are located closely to each other in the nuclear compartment enriched by the nuclear speckles. However, in KtyII locus knockout mice, EDC locus moved away from the KtyII locus flanking regions and nuclear speckles towards the nuclear periphery, which is associated with marked changes in …
-
Molecular Dissection of a Caenorhabditis Elegans Germ Granule Assembly Protein to Determine Its Role in Cell Development
… type of germ granule that localize to the nuclear periphery of developing germ cells and regulate mRNA expression. Proper P granule assembly is dependent on the PGL family of granule forming proteins, with PGL-1 serving as a key component containing two dimerization domains and a C-terminal …
-
Study of 3D Genome Organisation in Budding Yeast by Heterogeneous Polymer Simulations
… genes were found to be located closer to the nuclear periphery upon activation in the simulations. It was also shown that the determined distribution of proteins along the genome is crucial to achieve the correct genome organisation. Hence, the heterogeneous binding of proteins, which results …
-
PROTEASOME-DEPENDENT ENTRY OF HERPES SIMPLEX VIRUS
… the cytosol but prior to capsid arrival at the nuclear periphery. Proteasome-dependent viral entry was not reliant on host or viral protein synthesis. MG132, a peptide aldehyde that competitively inhibits the degradative activity of the proteasome, had a reversible inhibitory effect on herpes …
-
Mechanisms and functional significance of nuclear compartmentalization
… For better understanding of the effects of nuclear repositioning of a gene locus on gene expression, I investigated the physiological significance and mechanism of association of a gene locus with a nuclear sub-compartment. In chapter 1, I showed an actin dependent association of HSP70 …
-
The role of heterochromatin markers in lytic human herpesvirus egress and maturation
… remodeling as chromatin is marginalized to the nuclear periphery to accommodate viral replication and later to provide structural support during nuclear egress. However, the specific role of heterochromatin in these lytic infections remain unknown. In this thesis, I will show that host …
-
Genomic and microscopic dissection of nuclear compartmentalization and function
… for gene expression regulation. Abnormal nuclear organization is related to disease. Microscopy measurements of individual genes reveal a population-based, statistical gradient of increased gene activity from the nuclear periphery to the center. Sequencing-based methods (Hi-C, DamID) …
-
Investigating the DNA damage response induced by Typhoid Toxin
… novel γH2AX localisation was observed at the nuclear periphery (γH2AX rings) that failed to recruit DNA repair factors. This signified a non-canonical DDR that became the main focus of this project. This toxin-induced γH2AX ring pathway was shown to be replication-dependent: it was exacerbated …