Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"nuclear localization signals"”.
-
Characterization of Nuclear Localization Signals of Karyopherin-Mediated Nuclear Import
… Kap betas recognize their cargos through the nuclear localization signals (NLSs) and carry them into the nucleus. Recent structural and biochemical work on Kap beta2 (or Transportin) and its well-characterized hnRNP A1-NLS (or M9NLS) reveal that NLSs recognized by Kap beta2 are structurally …
-
Nuclear Localization of Proteins and Genome Editing in the Oomycete Phytophthora sojae
… of the most important intracellular activities, nuclear localization of proteins, and describe the characterization of nuclear localization signals (NLSs) in P. sojae. This focus stemmed from my early work on genome editing in P. sojae, when I discovered that conventional NLS signals from SV40 …
-
THE ROLE OF TIGHT JUNCTION PROTEIN ZO-2 IN THE NUCLEUS AND ITS LINK TO TRANSCRIPTION FACTORS OF THE SLUG/SNAIL FAMILY
… factor was shown to possess functional nuclear localization signals, nuclear export signal and zinc fingers that are important for DNA binding and nuclear retention. Furthermore, ZO-2 may also influence the sub-cellular localization of this transcription factor, for example in wound …
-
Shield and Shuttle: Importins as Protective Chaperones and the Nuclear Transport Mechanism of H2A-H2B
… that importins could have dual functions as 1) nuclear importers and 2) molecular chaperones. This thought stems back to characteristics of importins: they are negatively charged and shuttle positively charged DNA and RNA binding proteins prone to aggregation such as histones, ribosomal …
-
Recognition Mechanisms of Nuclear Localization and Export Signals
… recognize their substrates through either a nuclear localization or export signal depending on the direction of transport. Even though there are ten yeast import Karyopherin betas, for the past thirteen years there was only one well characterized nuclear localization signal, the classical …
-
A Proteomic Approach to Elucidating the Function of Picornavirus 2A Protease
… cycle, cellular metabolism, protein synthesis, nuclear and RNA transport across the nuclear pore complex, apoptosis, and innate immune responses. Picornaviruses are the most dominant human disease-causing viruses and present an excellent clinical target for research studies into their molecular …
-
Explorer l'impact de l'élimination de la charge nette cationique des signaux de localisation nucléaire (NLS) sur le ciblage par l'importine-α
… weight molecules to freely diffuse through nuclear pores, making it challenging to target. Nuclear transport is a vital component of normal functioning in eukaryotic cells. Specialized transport proteins called importins recognize nuclear localization signals (NLSs) on proteins in the …
-
Karyopherin alpha subtypes and porcine early stage embryo development
… karyopherin α/β heterodimer is an intracellular nuclear trafficking system that mediates nuclear import of proteins that bear classical nuclear localization signals (NLSs). Seven karyopherin α subtypes have been identified in the domestic pig, and while each of these karyopherin α subtypes is …
-
Gene Expression Control for Synthetic Patterning of Bacterial Populations and Plants
… reporters successfully expressed from its nuclear and chloroplast genomes. For targeting of bacteriophage T7 RNA polymerase to these cellular compartments, I functionally validated nuclear localization signals and chloroplast transit peptides. For spatiotemporal control of bacteriophage T7 …
-
Characterization of the Beta-Karyopherin Protein Ipo9 During Germ Cell Differentiation
… to work they need to access the nucleus, through nuclear trafficking machinery. The karyopherin family of proteins is responsible for nuclear import and export. The contribution of nuclear trafficking during gametogenesis is not well understood. Here we demonstrated that the well conserved …
-
Kap-[beta]2 Recognition of Cargoes Involved in Neurodegenerative/Neurodevelopmental Diseases
Karyopherin β2 is a nuclear-import receptor that transports many RNA-binding proteins into the nucleus by recognizing within the cargoes a unique nuclear-localization signal known as the PY-NLS. Several of Kapβ2 cargoes are mutated in neurological diseases. Two such proteins of interest to my …