Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"nuclear localization signal"”.
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The functional motifs for nucleocytoplasmic shuttling of C-terminal fragment of c-Met : A novel "pH-dependent' nuclear localization signal
… specific amino acid motif nor the responsible nuclear localization signal (NLS) has not been previously reported. Here, we report that two histidine residues separated by a 10-amino-acid spacer (H1068-H1079) located in the juxtamembrane region of c-Met function as a putative novel NLS. …
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Recognition Mechanisms of Nuclear Localization and Export Signals
… recognize their substrates through either a nuclear localization or export signal depending on the direction of transport. Even though there are ten yeast import Karyopherin betas, for the past thirteen years there was only one well characterized nuclear localization signal, the classical …
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Functional Study of Hemogen Knockout Mouse Model
… By generating a deletion mutant of the predicted nuclear localization signal, I conclude the amino acids 61 to 78 is a functional nuclear localization signal. Both the nuclear localization signal and the predicted coiled-coil domain were required for Hemgn to protect Ba/F3 cells from IL-3 …
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Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2
… and western blot analyzing cytoplasmic and nuclear fractions of expression cells showed targeting of SF2a, SF2b and their SR domains to the nuclei. SR domain, possibly required phosphorylation, is responsible for the nuclear localization signal.
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Mapping of the repression domain and the subcellular localization of the ABF-1 protein
… To complement these studies, the subcellular localization and nuclear localization signal of GFP-tagged ABF -1 proteins was performed using fluorescent microscopy. These studies suggest that ABF-1 exerts it function through the bHLH domain and the region of the nuclear localization signal lies …
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Investigation into the Akirin Subcellular Localization and Developmental Interactions
<p>Akirin is a highly conserved nuclear protein ubiquitously expressed in nonfungal eukaryotic species and is known to be involved in many biological processes such as embryonic muscle patterning and innate immune function. Akirin functionality is essential for the prevention of disease and …
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Molecular characterization of XvPer1, a novel antioxidant enzyme from the resurrection plant Xerophyta viscosa, and AC3, a LEA-like protein from Arabidopsis thaliana
… protein sequence also had a putative bipartite nuclear localization signal.
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Investigation of Akirin Nuclear Localization and the Molecular Interaction between Akirin and Taf4 in Drosophila
… we investigated the functionality of an Akirin Nuclear Localization Signal (NLS). Through calcium phosphate transfection of S2 cells and fluorescent imaging, we were able to compare wild-type full-length Akirin protein fused to the Venus fluorescent protein to full length Akirin::Venus fusion …
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Studies on the interaction of HSV-1 multifunctional protein ICP27 with the nuclear pore complex proteins
… levels. ICP27 contains both a nuclear localization signal and a nuclear export signal and shuttles between the nucleus and the cytoplasm. It interacts directly with various cellular proteins including REF and cellular mRNA export receptor TAP to export viral mRNAs. However other …
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Design of an in vitro assay to optimize assembly of nanoparticle-tagged nuclear import complexes
… importin-a and importin-3 mediate classical nuclear transport, an essential and highly conserved example of protein complex formation in eukaryotic cells. Together, these two proteins form a chaperone complex that recognizes a nuclear localization signal (NLS), which is a short peptide …
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Ehrlichia chaffeensis Type 1 Secreted Effector TRP47 is a Novel SUMOylated Nucleomodulin
… nucleomodulin with DNA-binding activity and host nuclear localization. Using a microfluidic chip peptide array, a SUMOylation site in the TRP47 N-terminal domain was identified. A SUMO-2 modification was further confirmed using an in vitro SUMOylation assay with recombinant TRP47, and native …
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Proteomic Analyses of HIV-1 Infected T-cells and the Functional Characterization of Cyclophilin B During HIV-1 Infection
… infection perturbs the host cell proteome. The nuclear compartment of cells is critical for HIV replication: integration of viral DNA into the host genome is essential to virus replication and pathogenesis; numerous host transcription factors are required for HIV-1 gene expression; and subtle …
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Characterizing the cargo binding and regulatory function of the tail domain in Ncd motor protein
… Ncd activity is regulated during interphase by nuclear sequestration. GFP-Ncd fusion proteins, containing full length Ncd, individual Ncd domains, or combinations of Ncd domains, were used to identify the presence of a nuclear localization signal (NLS) in the Ncd polypeptide. The nuclear …
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ZFP36L3: a Unique Member of the Tristetraprolin Family of RNA-Binding Tandem Zinc Finger Proteins
… of L3 deletion mutants, we determined that a nuclear localization signal that resides within the conserved tandem zinc finger domain was functional, although the C-terminal nuclear export sequence was non-functional. We then demonstrated that the unique repeat domain of L3 was responsible for …
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Innate immune modulation and pathogenic basis for porcine reproductive and respiratory syndrome virus
… The nsp1β subunit of nsp1 blocks the host mRNA nuclear export and this is one of the viral mechanisms to inhibit host antiviral protein translation. The functional motif for both IFN suppression and host mRNA nuclear retention were identified in nsp1β, and mutations in the motif results in the …
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Kap-[beta]2 Recognition of Cargoes Involved in Neurodegenerative/Neurodevelopmental Diseases
Karyopherin β2 is a nuclear-import receptor that transports many RNA-binding proteins into the nucleus by recognizing within the cargoes a unique nuclear-localization signal known as the PY-NLS. Several of Kapβ2 cargoes are mutated in neurological diseases. Two such proteins of interest to my …
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Analysis of nuclear transport signals of the human apurinic/apurimidinic endonuclease (APE1/REF1)
The nuclear localization signal (NLS) in human apurinic/apyrimidinic endonuclease (APE1), a key protein in both DNA base excision repair and transcriptional regulation, has not been analyzed in detail. We examined the role of specific residues in nuclear translocation of APE1, using green …
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AY-‐WB phytoplasma manipulations of host and non-‐host leafhopper interactions
… proteins (SAPs) identified, SAP11, carries a nuclear localization signal and accumulates in plant cell nuclei (Bai et al. 2009). SAP11 is shown to reduce production of plant defense hormone jasmonic acid (Sugio et al. 2011). Stable expression of SAP11 and 3 other SAPs in Arabidopsis increase …
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Examination of Brain-Fatty Acid Binding Protein Interaction with Hydrophobic Ligands and Micelles
… and protein-protein interactions. FABP7 bears a nuclear localization signal that allows ligand-dependent transport to the nucleus through the nuclear pore complex. Once in the nuclear pore complex, FABP7 can then exchange its cargo with transcription factors. FABP7 can also exchange cargo and …
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