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Showing 1 to 20 of 157 for “"nuclear localization"”.
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Recognition Mechanisms of Nuclear Localization and Export Signals
… recognize their substrates through either a nuclear localization or export signal depending on the direction of transport. Even though there are ten yeast import Karyopherin betas, for the past thirteen years there was only one well characterized nuclear localization signal, the classical …
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Characterization of Nuclear Localization Signals of Karyopherin-Mediated Nuclear Import
… Kap betas recognize their cargos through the nuclear localization signals (NLSs) and carry them into the nucleus. Recent structural and biochemical work on Kap beta2 (or Transportin) and its well-characterized hnRNP A1-NLS (or M9NLS) reveal that NLSs recognized by Kap beta2 are structurally …
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MSH2 nuclear localization and MMR apoptotic response to DNA damage.
… mechanism, dependent on the type of damage. Nuclear influx of MSH2 may be part of the repair-independent mechanism. Results from confocal microscopy and fluorescent linked immunosorbent assays showed both a corollary movement of MSH2 with the MMR repair protein EXO1, as well as an ability of …
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Interactions between Nova and brPTB Proteins in Alternative Splicing and Nuclear Localization
… selection. Nova proteins localize in distinct nuclear foci, but fail to co-localize with any of the known proteins that occupy sub-nuclear structural domains. Both endogenous and transfected brPTB and Nova proteins co-localize in these foci in neuroblastoma cell lines but not in non-neuronal …
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Nuclear Localization of Proteins and Genome Editing in the Oomycete Phytophthora sojae
… of the most important intracellular activities, nuclear localization of proteins, and describe the characterization of nuclear localization signals (NLSs) in P. sojae. This focus stemmed from my early work on genome editing in P. sojae, when I discovered that conventional NLS signals from SV40 …
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p53 nuclear localization control, and p53-dependent regulation of DNA repair gene transcripts
… genes. Chapter 2 addresses the regulation of nuclear localization of the p53 protein. Previous reports in the literature had suggested that the p53 negative regulator HDM2 was a nucleocytoplasmic shuttling protein that binds and carries p53 from the nucleus of the cell to the cytoplasm where …
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Investigation of Akirin Nuclear Localization and the Molecular Interaction between Akirin and Taf4 in Drosophila
… we investigated the functionality of an Akirin Nuclear Localization Signal (NLS). Through calcium phosphate transfection of S2 cells and fluorescent imaging, we were able to compare wild-type full-length Akirin protein fused to the Venus fluorescent protein to full length Akirin::Venus fusion …
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Calcium/Calmodulin Dependent Protein Kinase Type-II Associates with Flightless-I to Influence its Nuclear Localization
… (un-tagged) proteins. The association and localization of Fli-I are dependent on CaMK-II's activity state, although Fli-I is not a substrate of CaMK-II. When CaMK-II is inhibited, Fli-I translocates to the nucleus. Conversely when CaMK-II is artificially activated using a Ca2+ ionophore, …
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The functional motifs for nucleocytoplasmic shuttling of C-terminal fragment of c-Met : A novel "pH-dependent' nuclear localization signal
… specific amino acid motif nor the responsible nuclear localization signal (NLS) has not been previously reported. Here, we report that two histidine residues separated by a 10-amino-acid spacer (H1068-H1079) located in the juxtamembrane region of c-Met function as a putative novel NLS. …
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Analysis of nuclear transport signals of the human apurinic/apurimidinic endonuclease (APE1/REF1)
The nuclear localization signal (NLS) in human apurinic/apyrimidinic endonuclease (APE1), a key protein in both DNA base excision repair and transcriptional regulation, has not been analyzed in detail. We examined the role of specific residues in nuclear translocation of APE1, using green …
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Microtubule interactions and regulation of the mitotic kinesin-like protein-1 and kinesin-like calmodulin-binding protein
… native HsMKLP-1. To define the role of nuclear localization in regulating the activity of HsMKLP-1 during interphase, sequences directing nuclear localization of HsMKLP-1 were identified. Mutation of the nuclear localization sequence 799PNGSRKRR806 to 799PNGSRTSR806 or removal of AA's …
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Effects of Light on BDBT Puncta Formation in the Drosophila Eye Reveal Involvement of BDBT with Color Preference Behavior and Distinct Nuclear Localization Pathways for DBT and PER
… is needed for normal changes in subcellular localization of the circadian regulators PERIOD (PER) and DOUBLETIME (DBT) in the Drosophila eye. Finally, BDBT is demonstrated to have a role with the eye clock in altering circadian color preference in flies. The kinase DBT is a regulator of the …
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Examination of Brain-Fatty Acid Binding Protein Interaction with Hydrophobic Ligands and Micelles
… and protein-protein interactions. FABP7 bears a nuclear localization signal that allows ligand-dependent transport to the nucleus through the nuclear pore complex. Once in the nuclear pore complex, FABP7 can then exchange its cargo with transcription factors. FABP7 can also exchange cargo and …
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Investigation into the Akirin Subcellular Localization and Developmental Interactions
<p>Akirin is a highly conserved nuclear protein ubiquitously expressed in nonfungal eukaryotic species and is known to be involved in many biological processes such as embryonic muscle patterning and innate immune function. Akirin functionality is essential for the prevention of disease and …
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Ehrlichia chaffeensis Type 1 Secreted Effector TRP47 is a Novel SUMOylated Nucleomodulin
… nucleomodulin with DNA-binding activity and host nuclear localization. Using a microfluidic chip peptide array, a SUMOylation site in the TRP47 N-terminal domain was identified. A SUMO-2 modification was further confirmed using an in vitro SUMOylation assay with recombinant TRP47, and native …
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Functional Study of Hemogen Knockout Mouse Model
… By generating a deletion mutant of the predicted nuclear localization signal, I conclude the amino acids 61 to 78 is a functional nuclear localization signal. Both the nuclear localization signal and the predicted coiled-coil domain were required for Hemgn to protect Ba/F3 cells from IL-3 …
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Karyopherin alpha subtypes and porcine early stage embryo development
… karyopherin α/β heterodimer is an intracellular nuclear trafficking system that mediates nuclear import of proteins that bear classical nuclear localization signals (NLSs). Seven karyopherin α subtypes have been identified in the domestic pig, and while each of these karyopherin α subtypes is …
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Feedback Regulation of Wnt Signaling by Naked Cuticle (Nkd) During Drosophila Embryogenesis
… 30 amino acid motif is essential for Nkd nuclear localization and function. Substitution of the 30aa motif with a heterologous nuclear localization sequence (NLS) rescued some nkd mutants to adulthood. In support of Nkd's role in the nucleus, Nkd binds to Importin-alpha 3, an adaptor for …
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