Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"nuclear import"”.
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Characterization of Nuclear Localization Signals of Karyopherin-Mediated Nuclear Import
… beta) proteins in a Ran-dependent manner. Ten import Kap betas recognize their cargos through the nuclear localization signals (NLSs) and carry them into the nucleus. Recent structural and biochemical work on Kap beta2 (or Transportin) and its well-characterized hnRNP A1-NLS (or M9NLS) reveal …
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Structural and Biochmical Studies of Nuclear Import Factor Karyopherin Beta-2
Karyopherinbeta (Kapbeta ) proteins recognize nuclear localization and export signals (NLS/NES) and mediate the transport of macromolecules across the nuclear envelope, a process regulated by Ran GTPase through its nucleotide cycle. Diversity and few number of available signal sequences recognized …
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Nuclear import of histone fold motif containing heterodimers by importin 13
In der vorliegenden Arbeit wurde der nukleäre Import von zwei verwandten histone-fold Proteinpaaren, CHRAC-15/17 und p12/CHRAC-17, untersucht. Der CHRAC-15/17 Heterodimer ist dabei Bestandteil des so genannten chromatin accessibility complex (CHRAC), der sich aus SNF2H, der regulatorischen …
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Characterisation of mutants involved in Phytochrome A nuclear import and signal transduction
… to isolate and characterise Phytochrome A (phyA) nuclear transport mutants. The screening was performed in two steps. First, the mutagenised seedlings were selected on the basis of differences in hypocotyl length. In a second step, the selected putants were screened for altered localisation of …
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Investigating the nuclear import protein KPn߀1 as a cancer therapeutic target
… others reported the elevated expression of the nuclear import protein Karyopherin Beta 1 (Kpnβ1) in various cancers. The inhibition of Kpnβ1 by siRNA silencing inhibited the proliferation of cancer cells and induced cell death via apoptosis while having little effect on non-cancer cells. These …
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The role of Karyopherin β1 in the nuclear import of HIV-1 proteins
Includes abstract. Includes bibliographical references.
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Investigating a novel small molecule inhibitor of nuclear import as an anti-cancer approach
… others have reported elevated expression of the nuclear transporter Kpnβ1 in multiple cancers. Using the cervical cancer model, we showed that its inhibition using small-interfering RNA (siRNA) resulted in cancer cell death via apoptosis while sparing normal cells, suggesting it has potential as …
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Toxic PRn Poly-Dipeptides Encoded by the C9orf72 Repeat Expansion Block Nuclear Import and Export
… proteomics approach, demonstrating that the nuclear pore complex (NPC) as a major binding target. The biological significance of the PRn peptide-NPC interaction became apparent when we observed major defects in both mature mRNA export to the cytoplasm and protein import into the nucleus after …
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Modulating the expression and activity of the nuclear import protein, Karyopherin β1, in cancer cells
… found Karyopherin β1 (Kpnβ1), the primary nuclear import protein, to be significantly overexpressed in cervical cancer tissue. Further studies showed that inhibition of Kpnβ1 expression by siRNA resulted in cancer cell death, while non-cancer cells were only minimally affected. These …
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Design of an in vitro assay to optimize assembly of nanoparticle-tagged nuclear import complexes
… binding partners. Two chaperone proteins, importin-a and importin-3 mediate classical nuclear transport, an essential and highly conserved example of protein complex formation in eukaryotic cells. Together, these two proteins form a chaperone complex that recognizes a nuclear localization …
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Exploring the Early Events in the HIV-1 Life Cycle: From Post-Entry Restriction to Nuclear Import
… to the nucleus during a process known as viral nuclear import. Viral nuclear import is a key early step in the viral life cycle directly preceding integration that has to date been exploited as a target for antiretroviral therapy. Our recent studies indicate that a small stretch of sequence …
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Characterising the anticancer effects of a small molecule with potential to inhibit nuclear import via karyopherin beta1
… soluble transport proteins which are involved in nuclear-cytoplasmic trafficking. Studies have shown the involvement of Karyopherin proteins in nuclear pore assembly, nuclear membrane assembly and DNA replication. Since all these cell regulatory functions are critical for normal cell function, …
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The effect of inhibiting KPNB1-mediated nuclear import on cancer cell biology and inflammatory transcription factor signalling
… in our laboratory and others identified the nuclear import protein, Karyopherin β1 (KPNB1), to be overexpressed in various cancers and that inhibiting its expression blocks the proliferation of cancer cells. However, little is known about the potential role of KPNB1 in other cancer cell …
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Molecular characterization of components of the nuclear pore complex and the nuclear import system in Saccharomyces cerevisiae
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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Investigating the effect of a small molecule, Inhibitor of Nuclear Import (INI-43) as a pan-cancer treatment
Karyopherin beta 1 (KPNβ1) is a nuclear import protein that transports cargo molecules from the cytoplasm into the nucleus and plays an important role in other cellular processes such as mitosis and mitotic spindle assembly, DNA replication and cell cycle progression. Previous studies in our …
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Molecular Mechanism of Insulin Resistance: Role of mTOR Signaling Pathways
… In this study I initially set out to identify nuclear export signals in mTOR. A systematic scan of the mTOR sequence revealed 16 peptides conforming to the canonical leucine-rich nuclear export signal, of which 3 were found by reporter assays to contain LMB-sensitive and leucine-dependent …
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New Insights into the Roles of Human DNA Damage Checkpoint Protein ATR in the Regulation of Nucleotide Excision Repair and DNA Damage-Induced Cell Death
… activities reduced the UV-induced XPA import. Furthermore, we found that the ATR-directed XPA nuclear import happens primarily in the S phase of the cell cycle. In effort to determine the mechanism involved in the XPA nuclear import, we found that, in addition to the nuclear …
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The Regulation of Cellular Localization of Both Active and Inactive ERK1/2
… into the spatial control of ERK1/2 I examined nuclear import of ERK2 mutants, the interactions of ERK2 with karyopherins β1 and β2, and explored the roles of cytoskeletal elements, motor proteins and scaffolding complexes in the localization of ERK1/2. Mutation or dysregulation of the ERK1/2 …
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